Essential Stem Cell Methods
- 1st Edition - December 12, 2008
- Latest edition
- Editors: Robert Lanza, Irina Klimanskaya
- Language: English
This is a fast-moving field, and these detailed methods will help drive advances in stem cell research. The editors have hand selected step-by-step methods from researchers with… Read more
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Description
Description
This is a fast-moving field, and these detailed methods will help drive advances in stem cell research. The editors have hand selected step-by-step methods from researchers with extensive reputations and expertise.This volume, as part of the Reliable Lab Solutions series, delivers busy researchers a handy, time-saving source for the best methods and protocols in stem cells.
Key features
Key features
- Provides powerful research opportunities for those interested in perusing work in pluripotent stem cells, disease modeling, and other aspects of basic stem cell research
- Refines, organizes and updates popular methods from flagship series, Methods in Enzymology
- Highlights top downloads, enhanced with author tips and tricks and pitfalls to avoid
Readership
Readership
Researchers in biochemistry, cell and molecular biology, developmental biology and genetics.
Table of contents
Table of contents
Section 1: Organ-Derived Stem Cells
1. Neural Stem Cell Isolation and Characterization
Rodney L. Rietze and Brent A. Reynolds
2. Neural Stem Cells and Their Manipulation Evan Snyder
3. Retinal Stem Cells
Thomas A. Reh and Andy J. Fischer
4. Dental Pulp Stem Cells
He Liu, Stan Gronthos and Songtao Shi
5. Spermatogonial Stem Cells
Jon M. Oatley and Ralph L. Brinster
6. Stem Cells in the Lung
Xiaoming Liu, Ryan R. Driskell and John F. Engelhardt
7. Pancreatic Cells and Their Progenitors
Seth J. Salpeter and Yuval Dor
8. Pluripotent Stem Cells from Germ Cells Candace L. Kerr, Michael J. Shamblott and John D. Gearhart
9. Amniotic Fluid and Placental Stem Cells Dawn M. Delo, Paolo De Coppi, Georg Bartsch, Jr. and Anthony Atala
10. Cord Blood Stem and Progenitor Cells Mervin C. Yoder
11. Purification of Hematopoietic Stem Cells Using the Side Population
K.K. Lin and Margaret A. Goodell
12. Microarray Analysis of Stem Cells and Differentiation
Howard Y. Chang
13. Tissue Engineering Using Adult Stem Cells Daniel Eberli
14. Mesenchymal Stem Cells and Tissue Engineering
Nicholas W. Marion and Jeremy J. Mao
Section II: Embryonic Stem Cells
15. Murine Embryonic Stem Cells
Andras Nagy and Kristina Vintersten
16. Human Embryo Culture
Amparo Mercader, Diana Valbuena and Carlos Simón
17. Human Embryonic Stem Cells
Douglas Melton
18. Characterization and Evaluation of Human Embryonic Stem Cells
Chunhui Xu
19. Feeder-Free Culture of Human Embryonic Stem Cells
Michal Amit and Joseph Itskovitz-Eldor
20. Neural Stem Cells, Neurons, and Glia
Steven M. Pollard, Alex Benchoua and Sally Lowell
21. Hematopoietic Cells
Malcolm A.S. Moore, Jae-Hung Shieh and Gabsang Lee
22. Cardiomyocytes
Xiangzhong Yang, Xi-Min Guo, Chang-Yong Wang and X. Cindy Tian
23. Insulin-Producing Cells
Insa S. Schroeder, Gabriela Kania, Przemyslaw Blyszczuk
24. Transgene Expression and RNA Interference in Embryonic Stem Cells
Holm Zaehres and George Q. Daley
25. Lentiviral Vector-Mediated Gene Delivery into Human Embryonic Stem Cells
Michal Gropp and Benjamin Reubinoff
26. Engineering Embryonic Stem Cells with Recombinase Systems
Frank Schnütgen, A. Francis Stewart, Harald von Melchner and Konstantinos Anastassiadis
27. Tissue Engineering Using Embryonic Stem Cells Shahar Cohen, Lucy Leshanski and Joseph Itskovitz-Eldor
1. Neural Stem Cell Isolation and Characterization
Rodney L. Rietze and Brent A. Reynolds
2. Neural Stem Cells and Their Manipulation Evan Snyder
3. Retinal Stem Cells
Thomas A. Reh and Andy J. Fischer
4. Dental Pulp Stem Cells
He Liu, Stan Gronthos and Songtao Shi
5. Spermatogonial Stem Cells
Jon M. Oatley and Ralph L. Brinster
6. Stem Cells in the Lung
Xiaoming Liu, Ryan R. Driskell and John F. Engelhardt
7. Pancreatic Cells and Their Progenitors
Seth J. Salpeter and Yuval Dor
8. Pluripotent Stem Cells from Germ Cells Candace L. Kerr, Michael J. Shamblott and John D. Gearhart
9. Amniotic Fluid and Placental Stem Cells Dawn M. Delo, Paolo De Coppi, Georg Bartsch, Jr. and Anthony Atala
10. Cord Blood Stem and Progenitor Cells Mervin C. Yoder
11. Purification of Hematopoietic Stem Cells Using the Side Population
K.K. Lin and Margaret A. Goodell
12. Microarray Analysis of Stem Cells and Differentiation
Howard Y. Chang
13. Tissue Engineering Using Adult Stem Cells Daniel Eberli
14. Mesenchymal Stem Cells and Tissue Engineering
Nicholas W. Marion and Jeremy J. Mao
Section II: Embryonic Stem Cells
15. Murine Embryonic Stem Cells
Andras Nagy and Kristina Vintersten
16. Human Embryo Culture
Amparo Mercader, Diana Valbuena and Carlos Simón
17. Human Embryonic Stem Cells
Douglas Melton
18. Characterization and Evaluation of Human Embryonic Stem Cells
Chunhui Xu
19. Feeder-Free Culture of Human Embryonic Stem Cells
Michal Amit and Joseph Itskovitz-Eldor
20. Neural Stem Cells, Neurons, and Glia
Steven M. Pollard, Alex Benchoua and Sally Lowell
21. Hematopoietic Cells
Malcolm A.S. Moore, Jae-Hung Shieh and Gabsang Lee
22. Cardiomyocytes
Xiangzhong Yang, Xi-Min Guo, Chang-Yong Wang and X. Cindy Tian
23. Insulin-Producing Cells
Insa S. Schroeder, Gabriela Kania, Przemyslaw Blyszczuk
24. Transgene Expression and RNA Interference in Embryonic Stem Cells
Holm Zaehres and George Q. Daley
25. Lentiviral Vector-Mediated Gene Delivery into Human Embryonic Stem Cells
Michal Gropp and Benjamin Reubinoff
26. Engineering Embryonic Stem Cells with Recombinase Systems
Frank Schnütgen, A. Francis Stewart, Harald von Melchner and Konstantinos Anastassiadis
27. Tissue Engineering Using Embryonic Stem Cells Shahar Cohen, Lucy Leshanski and Joseph Itskovitz-Eldor
Product details
Product details
- Edition: 1
- Latest edition
- Published: October 13, 2011
- Language: English
About the editors
About the editors
RL
Robert Lanza
Robert Lanza is an American scientist and author whose research spans the range of natural science, from biology to theoretical physics. TIME magazine recognized him as one of the “100 Most Influential People in the World,” and Prospect magazine named him one of the Top 50 “World Thinkers.” He has hundreds of scientific publications and over 30 books, including definitive references in the fields of stem cells, tissue engineering, and regenerative medicine. He’s a former Fulbright Scholar and studied with polio-pioneer Jonas Salk and Nobel laureates Gerald Edelman (known for his work on the biological basis of consciousness) and Rodney Porter. He also worked closely (and co-authored papers in Science on self-awareness and symbolic communication) with noted Harvard psychologist BF Skinner. Dr. Lanza was part of the team that cloned the world’s first human embryo, the first endangered species, and published the first-ever reports of pluripotent stem cell use in humans.
Affiliations and expertise
Astellas Institute for Regenerative Medicine, Westborough, MA, USAIK
Irina Klimanskaya
Affiliations and expertise
Advance Cell Technology, Worcester, MA, USA