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Enzymes
Biochemistry, Biotechnology, Clinical Chemistry
- 2nd Edition - April 4, 2007
- Authors: T Palmer, P L Bonner
- Language: English
- Paperback ISBN:9 7 8 - 1 - 9 0 4 2 7 5 - 2 7 - 5
- eBook ISBN:9 7 8 - 0 - 8 5 7 0 9 - 9 9 2 - 1
In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student… Read more
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Request a sales quote- Provides an introduction to enzymology and a balanced account of the theoretical and applied aspects of the subject
- Discusses techniques such as membrane chromatography, aqueous phase partitioning and engineering recombinant proteins for purification
- Includes a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy
Part 1: Structure and Function of Enzymes
1: An Introduction to Enzymes
- 1.1 WHAT ARE ENZYMES?
- 1.2 A BRIEF HISTORY OF ENZYMES
- 1.3 THE NAMING AND CLASSIFICATION OF ENZYMES
- SUMMARY OF CHAPTER 1
- PROBLEMS
2: The Structure of Proteins
- 2.1 INTRODUCTION
- 2.2 AMINO ACIDS, THE BUILDING BLOCKS OF PROTEINS
- 2.3 THE BASIS OF PROTEIN STRUCTURE
- 2.4 THE DETERMINATION OF PRIMARY STRUCTURE
- 2.5 THE DETERMINATION OF PROTEIN STRUCTURE BY X-RAY CRYSTALLOGRAPHY
- 2.6 THE INVESTIGATION OF PROTEIN STRUCTURE IN SOLUTION
- SUMMARY OF CHAPTER 2
- PROBLEMS
3: The Biosynthesis and Properties of Proteins
- 3.1 THE BIOSYNTHESIS OF PROTEINS
- 3.2 THE PROPERTIES OF PROTEINS
- SUMMARY OF CHAPTER 3
- PROBLEMS
4: Specificity of Enzyme Action
- 4.1 TYPES OF SPECIFICITY
- 4.2 THE ACTIVE SITE
- 4.3 THE FISCHER ‘LOCK-AND-KEY’ HYPOTHESIS
- 4.4 THE KOSHLAND ‘INDUCED-FIT’ HYPOTHESIS
- 4.5 HYPOTHESES INVOLVING STRAIN OR TRANSITION-STATE STABILIZATION
- 4.6 FURTHER COMMENTS ON SPECIFICITY
- SUMMARY OF CHAPTER 4
5: Monomeric and Oligomeric Enzymes
- 5.1 MONOMERIC ENZYMES
- 5.2 OLIGOMERIC ENZYMES
- SUMMARY OF CHAPTER 5
Part 2: Kinetic and Chemical Mechanisms of Enzyme-Catalysed Reactions
6: An Introduction to Bioenergetics, Catalysis and Kinetics
- 6.1 SOME CONCEPTS OF BIOENERGETICS
- 6.2 FACTORS AFFECTING THE RATES OF CHEMICAL REACTIONS
- 6.3 KINETICS OF UNCATALYSED CHEMICAL REACTIONS
- 6.4 KINETICS OF ENZYME-CATALYSED REACTIONS: AN HISTORICAL INTRODUCTION
- 6.5 METHODS USED FOR INVESTIGATING THE KINETICS OF ENZYME-CATALYSED REACTIONS
- 6.6 THE NATURE OF ENZYME CATALYSIS
- SUMMARY OF CHAPTER 6
- PROBLEMS
7: Kinetics of Single-Substrate Enzyme-Catalysed Reactions
- 7.1 THE RELATIONSHIP BETWEEN INITIAL VELOCITY AND SUBSTRATE CONCENTRATION
- 7.2 RAPID-REACTION kINETICS
- 7.3 THE kING AND ALTMAN PROCEDURE
- SUMMARY OF CHAPTER 7
- PROBLEMS
8: Enzyme Inhibition
- 8.1 INTRODUCTION
- 8.2 REVERSIBLE INHIBITION
- 8.3 IRREVERSIBLE INHIBITION
- SUMMARY OF CHAPTER 8
- PROBLEMS
9: Kinetics of Multi-Substrate Enzyme-Catalysed Reactions
- 9.1 EXAMPLES OF POSSIBLE MECHANISMS
- 9.2 STEADY-STATE KINETICS
- 9.3 INVESTIGATION OF REACTION MECHANISMS USING STEADY-STATE METHODS
- 9.4 INVESTIGATION OF REACTION MECHANISMS USING NON-STEADY-STATE METHODS
- SUMMARY OF CHAPTER 9
- PROBLEMS
10: The Investigation of Active Site Structure
- 10.1 THE IDENTIFICATION OF BINDING SITES AND CATALYTIC SITES
- 10.2 THE INVESTIGATION OF THE THREE-DIMENSIONAL STRUCTURES OF ACTIVE SITES
- SUMMARY OF CHAPTER 10
- PROBLEM
11: The chemical nature of enzyme catalysis
- 11.1 AN INTRODUCTION TO REACTION MECHANISMS IN ORGANIC CHEMISTRY
- 11.2 MECHANISMS OF CATALYSIS
- 11.3 MECHANISMS OF REACTIONS CATALYSED BY ENZYMES WITHOUT COFACTORS
- 11.4 METAL-ACTIVATED ENZYMES AND METALLOENZYMES
- 11.5 THE INVOLVEMENT OF COENZYMES IN ENZYME-CATALYSED REACTIONS
- SUMMARY OF CHAPTER 11
12: The Binding of Ligands to Proteins
- 12.1 INTRODUCTION
- 12.2 THE BINDING OF A LIGAND TO A PROTEIN HAVING A SINGLE LIGAND-BINDING SITE
- 12.3 COOPERATIVITY
- 12.4 POSITIVE HOMOTROPIC COOPERATIVITY AND THE HILL EQUATION
- 12.5 THE ADAIR EQUATION FOR THE BINDING OF A LIGAND TO A PROTEIN HAVING TWO BINDING SITES FOR THAT LIGAND
- 12.6 THE ADAIR EQUATION FOR THE BINDING OF A LIGAND TO A PROTEIN HAVING THREE BINDING SITES FOR THAT LIGAND
- 12.7 THE ADAIR EQUATION FOR THE BINDING OF A LIGAND TO A PROTEIN HAVING FOUR BINDING SITES FOR THAT LIGAND
- 12.8 INVESTIGATION OF COOPERATIVE EFFECTS
- 12.9 THE BINDING OF OXYGEN TO HAEMOGLOBIN
- SUMMARY OF CHAPTER 12
- PROBLEMS
13: Sigmoidal Kinetics and Allosteric Enzymes
- 13.1 INTRODUCTION
- 13.2 THE MONOD-WYMAN-CHANGEUX (MWC) MODEL
- 13.3 THE KOSHLAND-NÉMETHY-FILMER (KNF) MODEL
- 13.4 DIFFERENTIATION BETWEEN MODELS FOR COOPERATIVE BINDING IN PROTEINS
- 13.5 SIGMOIDAL KINETICS IN THE ABSENCE OF COOPERATIVE BINDING
- SUMMARY OF CHAPTER 13
- PROBLEMS
14: The Significance of Sigmoidal Behaviour
- 14.1 THE PHYSIOLOGICAL IMPORTANCE OF COOPERATIVE OXYGEN-BINDING BY HΔEMOGLOBIN
- 14.2 ALLOSTERIC ENZYMES AND METABOLIC REGULATION
- SUMMARY OF CHAPTER 14
Part 3: Application of Enzymology
15: Investigation of Enzymes in Biological Preparations
- 15.1 CHOICE OF PREPARATION FOR THE INVESTIGATION OF ENZYME CHARACTERISTICS
- 15.2 ENZYME ASSAY
- 15.3 INVESTIGATION OF SUB-CELLULAR COMPARTMENTATION OF ENZYMES
- SUMMARY OF CHAPTER 15
- PROBLEM
16: Extraction and Purification of Enzymes
- 16.1 EXTRACTION OF ENZYMES
- 16.2 PURIFICATION OF ENZYMES
- 16.3 DETERMINATION OF MOLECULAR WEIGHTS OF ENZYMES
- SUMMARY OF CHAPTER 16
- PROBLEM
17: Enzymes as Analytical Reagents
- 17.1 THE VALUE OF ENZYMES AS ANALYTICAL REAGENTS
- 17.2 PRINCIPLES OF ENZYMATIC ANALYSIS
- 17.3 HANDLING ENZYMES AND COENZYMES
- SUMMARY OF CHAPTER 17
- PROBLEMS
18: Instrumental Techniques Available for Use in Enzymatic Analysis
- 18.1 PRINCIPLES OF THE AVAILABLE DETECTION TECHNIQUES
- 18.2 AUTOMATION IN ENZYMATIC ANALYSIS
- 18.3 HIGH-THROUGHPUT ASSAYS (HTA)
- SUMMARY OF CHAPTER 18
19: Applications of Enzymatic Analysis in Medicine, Forensic Science and Industry
- 19.1 APPLICATIONS IN MEDICINE
- 19.2 APPLICATIONS IN FORENSIC SCIENCE
- 19.3 APPLICATIONS IN INDUSTRY
- SUMMARY OF CHAPTER 19
20: Biotechnological Applications of Enzymes
- 20.1 LARGE-SCALE PRODUCTION AND PURIFICATION OF ENZYMES
- 20.2 IMMOBILIZED ENZYMES
- 20.3 ENZYME UTILIZATION IN INDUSTRY
- SUMMARY OF CHAPTER 20
- PROBLEMS
21: Genomics, Proteomics and Bioinformatics
- 21.1 ENZYMES AND RECOMBINANT DNA TECHNOLOGY
- 21.2 PROTEOMICS
- 21.3 ENZYMES AND BIOINFORMATICS
- SUMMARY OF CHAPTER 21
- PROBLEMS
Answers to ProblemsAbbreviations
- No. of pages: 440
- Language: English
- Edition: 2
- Published: April 4, 2007
- Imprint: Woodhead Publishing
- Paperback ISBN: 9781904275275
- eBook ISBN: 9780857099921
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