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Preparation, Characterisation, Properties and Applications
1st Edition - May 16, 2015
Author: Chandra S. Pundir
9 7 8 - 0 - 3 2 3 - 3 8 9 1 3 - 6
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This book is the first book in English on nanotechnology and nanomaterials integrating with enzymatic systems, with a focus on nanoparticles and biological applications. It covers… Read more
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This book is the first book in English on nanotechnology and nanomaterials integrating with enzymatic systems, with a focus on nanoparticles and biological applications. It covers comprehensively the relevant topics to understand the development of enzyme nanoparticles as it relates to the complicated structures of enzyme nanoparticles and their functionalization and immobilization on to various supports. The preparation of enzyme nanoparticles, their kinetic properties and applications after immobilization of the immobilized enzyme nanoparticles is described. The use of colour images in all formats of the book will improve the understanding of the topics covered. The book offers an integration of Enzymology and Nanotechnology and provides the latest information on preparation of enzyme nanoparticles, their characterization, their functionalization and immobilization on to various supports and thereafter their kinetic properties and applications in various industries with special reference to Biosensor Technology.
Focus on enzyme nanotechnology, given the wide appeal of enzymes for diagnostics, therapy and biocatalysis
Provision of a general background to the topic, but also a detailed description of synthesis, preparation and applications
Researchers in industry and academia, in the fields of Nanoscience and Nanotechnology, Advanced Materials research, Biomedical Engineering, Biotechnology, Bio-Nanotechnology, Biosensor Technology, Nano-Medicine, Pharmacy, Dentistry, Veterinary Medicine, Life Sciences, Enzyme Catalysis and Biochemistry; Graduate and post-graduate students in Biochemistry, Biotechnology, Nanoscience and Nanotechnology, Materials Science, Biosensor Technology and Nano-Medicine courses
About the author
Chapter 1. Introduction to Enzyme and Nanotechnology
What Are Nanoparticles and Nanotechnology?
What Are Enzymes and Their Aggregates?
What Are Enzyme Nanoparticles?
How Are Nanoparticles of Enzymes Prepared?
What Does This Book Describe?
Chapter 2. Preparation of Enzyme Nanoparticles
Method of ENPs Formation
Chemistry of Formation and Functionalization of ENPs
Chapter 3. Characterization of Enzyme Nanoparticles
Chapter 4. Immobilization of Enzyme Nanoparticles
Immobilization of HRP NPs Aggregates
Immobilization of GOD NPs Aggregates
Immobilization of ChOx NPs Aggregates
Immobilization of Uricase NPs Aggregates
Chemistry of Immobilization of ENPs
Confirmation of Immobilization of ENPs
Conjugation Yield and % Retention of Immobilized ENPs
Chapter 5. Kinetic Properties of Enzyme Nanoparticles
Optimum Temperature and Thermostability
Chapter 6. Applications of Enzyme Nanoparticles
HorseRadish Peroxidase (HRP) NPs-Based Biosensor
Glucose Oxidase (GOD) NPs-Based Biosensors
Cholesterol Oxidase (ChOx) NPs-Based Biosensor
Uricase NPs-Based Biosensor
Chapter 7. Future Development in Enzyme Nanoparticles
Appendix. Enzymes Used in Day-to-Day Life
Statistical Formulae for Precision and Correlation
No. of pages: 82
Published: May 16, 2015
Imprint: William Andrew
Paperback ISBN: 9780323389136
eBook ISBN: 9780323389297
Chandra S. Pundir
Affiliations and expertise
Dept. of Biochemistry, Maharshi Dayanand University, Rohtak, Haryana, India