Enzyme Immunodiagnosis

Preface1 Introduction Text2 Preparation of Immunoglobulins, Antibodies, or Fab Fragments A. Immunization B. Isolation of Immunoglobulins C. Preparation of Fab Fragments3 Conjugation of Enzymes A. Purification of Enzymes B. Selected Procedures of Conjugation4 Principles of the Design of Enzyme Immunoassays A. Formulation of Methodology B. Solid Phases Used in Enzyme Immunoassays C. Operational Details of Enzyme Immunoassay Procedures D. Interpretation of Results Obtained with Enzyme Immunoassays5 Enzyme Immunohistochemical Methods A. Introduction B. Fixation of Tissue C. Detection of Antigens at the Light Microscopic Level D. Double Immunoenzymatic Labeling E. Nonspecific Staining F. Ultrastructural Localization of Antigens6 Enzyme Immunoassays After Immunoblotting A. Technical Approaches B. Immunoblotting Applications in Diagnosis and Research C. Conclusion7 The Use of Polyclonal or Monoclonal Antibodies in Enzyme Immunoassays A. Variation in Immune Response B. The Concepts of Affinity, Avidity, Cross-Reactivity, and Specificity C. The Consequences of the Production of Monoclonal Antibodies for Affinity and Specificity D. Unusual Properties of Monoclonal Antibodies E. Conclusion8 Homogeneous Enzyme Immunoassays for the Detection of Drugs A. Introduction B. Assay Design and Protocol C. Qualitative and Semiquantitative Detection of Drugs9 The Value of Enzyme Immunohistochemistry in Pathology, Oncology, and Histology A. Enzyme Immunoassays in Diagnostic Pathology B. Enzyme Immunohistochemical Detection of Antigens in Oncology C. Immunoperoxidase Application in Histology10 Enzyme Immunoassays for Infectious Diseases A. Introduction B. Enzyme Immunohistochemistry of Infected Cells and Tissue from Cultures C. The Use of Polyclonal Antisera or Monoclonal Antibodies in Enzyme Immunoassays for Infectious Pathogens D. Applications of Enzyme Immunoassays in Parasitology E. Applications of EIA in Bacteriology, Chlamydiology, and Rickettsiology F. Enzyme Immunoassays in Virology and for the Diagnosis of Viral Diseases11 Automation in Enzyme Immunoassays and Commercial Kits A. Total Automation B. Semiautomation C. Commercial Kits for Enzyme Immunoassays12 Screening of Recombinant DNA Expression Libraries A. Construction of Recombinant Transformants B. Immunological Screening of Large cDNA Expression Libraries13 Vaccine Assessment and Standardization A. Safety and Efficacy of Prospective Vaccines B. Vaccines: Quality Assessment and Standardization14 Future Prospects of Enzyme Immunodiagnosis TextAcknowledgmentsBibliographyIndex