Enzyme Engineering and Evolution: General Methods

1. In vivo site-directed recombination (SDR): An efficient tool to reveal beneficial epistasis

2. A beginner's guide to molecular dynamics simulations and the identification of cross-correlation networks for enzyme engineering

3. Exploration of enzyme diversity: High-throughput techniques for protein production and microscale biochemical characterization

4. Emulsion-based directed evolution of enzymes and proteins in yeast

5. Remodeling enzyme active sites by stepwise loop insertion

6. Consensus Finder web tool to predict stabilizing substitutions in proteins

7. The use of consensus sequence information to engineer stability and activity in proteins

8. Structure-guided rational design of the substrate specificity and catalytic activity of an enzyme

9. Biological fitness landscapes by deep mutational scanning

10. Focused rational iterative site-specific mutagenesis (FRISM)

11. Statistical analysis of mutational epistasis to reveal intramolecular interaction networks in proteins

12. Machine learning-assisted enzyme engineering

13. Ultrahigh throughput screening for enzyme function in droplets