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Current Topics in Bioenergetics
Volume 13
- 1st Edition - June 28, 2014
- Editor: C. P. Lee
- Language: English
- Paperback ISBN:9 7 8 - 1 - 4 8 3 2 - 0 3 5 4 - 6
- eBook ISBN:9 7 8 - 1 - 4 8 3 2 - 1 6 9 7 - 3
Current Topics in Bioenergetics, Volume 13 presents the biophysical and biochemical approaches that are valuable in some systems and that may be readily be applied to others. This… Read more
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Request a sales quoteCurrent Topics in Bioenergetics, Volume 13 presents the biophysical and biochemical approaches that are valuable in some systems and that may be readily be applied to others. This book discusses the function and structure relationship of energy-transducing membranes that has played an essential role in the progress and development in the field of bioenergetics. Organized into six chapters, this volume begins with an overview of the bioenergetics processes that require a molecular description of the structure and reaction mechanisms of the individual energy-transducing catalysts. This text then provides an understanding to the methodology for initiation of an electron paramagnetic resonance (EPR) investigation in this field of research. This book discusses as well the developments in spin label EPR techniques. The final chapter deals with immunological techniques that are used for studying the biochemistry and molecular biology of membrane proteins. This book is a valuable resource for physiologists, biologists, and biochemists.
Contributors
Preface
Contents of Previous Volumes
Determination of the Proton Electrochemical Gradient Across Biological Membranes
I. Introduction
II. General Considerations
III. Determination of Δψ and ΔpH by Ion Distribution
IV. Determination of Δψ and ΔpH with Other Methods
V. Utilization of ΔμH Measurements
Conclusion
References
Application of Electron Paramagnetic Resonance in the Study of Iron-Sulfur Clusters in Energy-Transducing Membranes
I. Introduction
II. The EPR Properties of Fe-S Clusters
III. Characterization of EPR Signals in Energy-Transducing Membranes
IV. Quantification of Signals
V. Kinetic Measurements
VI. Orientation of Fe-S Clusters in Membranes
VII. Concluding Remarks
References
Recent Developments in Spin Label EPR Methodology for Biomembrane Studies
I. Introduction
II. Nitroxide Spin Labels
III. Conventional EPR
IV. Saturation Transfer EPR
V. Pulse (Time Domain) EPR
VI. Prospects for Future Applications
References
Probing Structure and Motion of the Mitochondrial Cytochromes
I. Introduction
II. An Overview of Mitochondrial Components
III. Complex III: Cytochrome bC1
IV. Cytochrome c
V. Complex IV or Cytochrome c Oxidase
VI. ADP/ATP Translocator
VII. Conclusions and Further Questions
References
Enzyme-Catalyzed Oxygen Exchange Reactions and Their Implications for Energy Coupling
I. Introduction
II. Basic Concepts and Terminology
III. Enzyme-Catalyzed Oxygen Exchanges
IV. Epilogue
References
Use of Immunological Techniques to Study Membrane Proteins
I. Introduction
II. Preparing Antibodies against Membrane Proteins
III. Applications of Antibodies to the Study of Membrane Proteins
IV. Conclusions
References
Index
- No. of pages: 300
- Language: English
- Edition: 1
- Published: June 28, 2014
- Imprint: Academic Press
- Paperback ISBN: 9781483203546
- eBook ISBN: 9781483216973
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