Current Laboratory Techniques in Rabies Diagnosis, Research and Prevention, Volume 1

1st Edition - October 30, 2018
Editors: Charles Rupprecht, Thirumeni Nagarajan
Language: English
Hardback ISBN:
9 7 8 - 0 - 1 2 - 8 0 0 0 1 4 - 4
Paperback ISBN:
9 7 8 - 0 - 1 2 - 8 1 0 3 1 7 - 3
eBook ISBN:
9 7 8 - 0 - 1 2 - 8 0 0 4 6 5 - 4

Laboratory Techniques in Rabies Diagnosis, Research and Prevention provides a basic understanding of the current trends in rabies. It establishes a new facility for rabies su… Read more

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Laboratory Techniques in Rabies Diagnosis, Research and Prevention provides a basic understanding of the current trends in rabies. It establishes a new facility for rabies surveillance, vaccine and antibody manufacturing. It offers clarity about the choice of laboratory methods for diagnosis and virus typing, of systems for producing monoclonal and polyclonal antibodies and of methods for testing potency of vaccines and antibodies.

The book covers advancements in the classical methods described as well as recent methods and approaches pertaining to rabies diagnosis and research.

Foreword
List of Contributors
Part One: Introduction
- Chapter One. Basic Facts about Lyssaviruses
  - 1.1 Introduction
  - 1.2 Virion and Genome Organization
  - 1.3 Phylogeny and Serologic Cross-Reactivity of Lyssaviruses
  - 1.4 Host Range
  - 1.5 Pathobiology
  - References
Part Two: Rabies Diagnosis
- Section A: Demonstration of Viral Subunits and Antigens
  - Chapter Two. Demonstration of Lyssavirus Antigens by a Direct Rapid Immunohistochemical Test
    - 2.1 Introduction
    - 2.2 Materials
    - 2.3 Methods
    - 2.4 Discussion
    - References
  - Chapter Three. Demonstration of Rabies Virus Antigens by a Latex Agglutination Test
    - 3.1 Introduction
    - 3.2 Materials
    - 3.3 Methods
    - 3.4 Discussion
    - References
  - Chapter Four. Rabies Diagnosis: Demonstration of Viral Antigens by Flow Cytometry
    - 4.1 Introduction
    - 4.2 Materials
    - 4.3 Methods
    - 4.4 Discussion
    - Acknowledgments
    - References
  - Chapter Five. Demonstration of Rabies Virus Antigens by an Immunochromatographic Strip Test
    - 5.1 Introduction
    - 5.2 Materials
    - 5.3 Methods
    - 5.4 Discussion
    - References
- Section B: Demonstration of Viral Nucleic Acids
  - Chapter Six. Demonstration of African Lyssavirus RNA with Real-Time Polymerase Chain Reaction
    - 6.1 Introduction
    - 6.2 Materials
    - 6.3 Methods
    - 6.4 Discussion
    - References
  - Chapter Seven. Real-Time Quantitative Polymerase Chain Reaction for the Demonstration of Lyssavirus Nucleic Acid
    - 7.1 Real-Time Quantitative Polymerase Chain Reaction for the Demonstration of Lyssavirus Nucleic Acid
    - 7.2 Methodology
    - 7.3 Discussion
    - Acknowledgments
    - References
  - Chapter Eight. Reverse Transcription-Loop-Mediated Isothermal Amplification System for the Detection of Rabies Virus
    - 8.1 Introduction
    - 8.2 Materials
    - 8.3 Methods
    - 8.4 Discussion
    - Acknowledgments
    - References
  - Chapter Nine. Detection of Viral Nucleic Acids by In Situ Hybridization
    - 9.1 Introduction
    - 9.2 Materials
    - 9.3 Methods
    - 9.4 Discussion
    - Acknowledgments
    - References
  - Chapter Ten. Genetic Characterization via Pyrosequencing
    - 10.1 Introduction
    - 10.2 Materials
    - 10.3 Methods
    - 10.4 Discussion
    - Acknowledgments
    - References
- Section C: Demonstration of Viral Antibodies and Immune Complexes
  - Chapter Eleven. Demonstration of Immune Complexes by Capture Enzyme-Linked Immunosorbent Assay
    - 11.1 Introduction
    - 11.2 Materials
    - 11.3 Methods
    - 11.4 Discussion
    - Acknowledgments
    - References
  - Chapter Twelve. Demonstration of Viral Antibodies by an Immunochromatographic Strip Test
    - 12.1 Introduction
    - 12.2 Materials
    - 12.3 Methods
    - 12.4 Discussion
    - Acknowledgments
    - References
  - Chapter Thirteen. Rabies Diagnosis: Demonstration of Viral Antibodies by Flow Cytometry
    - 13.1 Introduction
    - 13.2 Materials
    - 13.3 Methods
    - 13.4 Discussion
    - Acknowledgments
    - References
  - Chapter Fourteen. Demonstration of Rabies Antibody by a Latex Agglutination Test
    - 14.1 Introduction
    - 14.2 Materials
    - 14.3 Methods
    - 14.4 Discussion
    - References
  - Chapter Fifteen. Demonstration of Viral Antibodies by Pseudotype Virus Neutralization
    - 15.1 Introduction
    - 15.2 Materials
    - 15.3 Methods
    - 15.4 Discussion
    - Acknowledgments
    - References
- Section D: Typing/Differentiation of Lyssaviruses
  - Chapter Sixteen. Sanger Sequencing of Lyssaviruses
    - 16.1 Background
    - 16.2 Methodology
    - 16.3 Discussion
    - Acknowledgments
    - References
  - Chapter Seventeen. Next Generation Sequencing of Lyssaviruses
    - 17.1 Background
    - 17.2 Methodology
    - 17.3 Discussion
    - Acknowledgments
    - References
  - Chapter Eighteen. Genetic Characterization of Rabies Viruses by In Situ Hybridization
    - 18.1 Introduction
    - 18.2 Materials
    - 18.3 Methods
    - 18.4 Discussion
    - Acknowledgments
    - References
  - Chapter Nineteen. Oligonucleotide Microarray: Applications for Lyssavirus Speciation
    - 19.1 Introduction
    - 19.2 Methodology
    - 19.3 Discussion
    - Acknowledgments
    - References
  - Chapter Twenty. Demonstration of Lyssavirus Nucleic Acids by Pyrosequencing
    - 20.1 Introduction
    - 20.2 Materials
    - 20.3 Methods
    - 20.4 Discussion
    - Acknowledgments
    - References
- Section E: Demonstration of Viral-Induced Changes in Tissues/Organs
  - Chapter Twenty One. Rabies Diagnosis: MR Imaging
    - 21.1 Introduction
    - 21.2 Materials
    - 21.3 Methods
    - 21.4 Discussion
    - Acknowledgments
    - References
Part Three: Rabies Biologics
- Section F: Rabies Vaccines for Humans or Other Animals
  - Chapter Twenty Two. Attenuated Vaccines for Veterinary Use
    - 22.1 Introduction
    - 22.2 Materials
    - 22.3 Methods
    - 22.4 Discussion
    - References
  - Chapter Twenty Three. Pox Viral Vectored Vaccines for Rabies
    - 23.1 Introduction
    - 23.2 Materials
    - 23.3 Methods
    - 23.4 Discussion
    - References
  - Chapter Twenty Four. Recombinant Rabies Virus Vaccines
    - 24.1 Introduction
    - 24.2 Materials, Methods, and Results
    - 24.3 Discussion
    - References
  - Chapter Twenty Five. Adenoviral Vectors as Vaccine Carriers for Prevention of Rabies
    - 25.1 Introduction
    - 25.2 Basic Characteristics of Ad Viruses
    - 25.3 Types of Ad Vectors
    - 25.4 Transgene Product for Rabies Vaccines
    - 25.5 Construction of Ad Vectors
    - 25.6 Ad Virus Rescue and Expansion
    - 25.7 Quality Control of Ad Vectors
    - 25.8 Discussion
    - References
- Section G: Rabies Antibodies/Fragments
  - Chapter Twenty Six. Production of Human Monoclonal Antibodies using Mammalian Expression Systems
    - 26.1 Introduction
    - 26.2 Materials
    - 26.3 Methods
    - Acknowledgments
    - References
  - Chapter Twenty Seven. Generation of Immune Globulin Single Variable Domains by Display Technologies
    - 27.1 Introduction
    - 27.2 Materials
    - 27.3 Methods
    - 27.4 Discussion
    - 27.5 Experimental Tips
    - Acknowledgments
    - References
Part Four: Appendix
- Appendix A
- Appendix B
- Appendix C. Filter Paper Technology for Sampling, Storage, and Shipment of Rabies Suspect Samples for the Identification of Viral Nucleic Acids
  - C.1 Introduction
  - C.2 Materials
  - C.3 Methods
  - C.4 Discussion
  - Acknowledgments
  - References
Index

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

Health

Current Laboratory Techniques in Rabies Diagnosis, Research and Prevention, Volume 1

Fuel your confidence!

Charles Rupprecht

Thirumeni Nagarajan

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