CRISPR-Cas System in Translational Biotechnology

1st Edition - November 16, 2023
Editors: Swati Joshi, Digvijay Verma, Ravi Kr. Gupta
Language: English
Paperback ISBN:
9 7 8 - 0 - 3 2 3 - 9 1 8 0 8 - 4
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 9 7 2 2 8 - 4

CRISPR-Cas System in Translational Biotechnology discusses applied and translational aspects of the CRISPR-Cas technology. The book bridges the gap between theoretical knowledge… Read more

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CRISPR-Cas System in Translational Biotechnology discusses applied and translational aspects of the CRISPR-Cas technology. The book bridges the gap between theoretical knowledge and practical solutions surrounding this emerging and impactful technology in several academic and industrial fields. It is split in five sections: CRISP-Cas fundamentals and advancements; CRISP-Cas in medical biotechnology; CRISP-Cas in environmental biotechnology; CRISP-Cas in food biotechnology; and biosafety, patents and commercialization of CRISP-Cas technology. Written by experts from diverse backgrounds, the content covers the subject and its impact in multiple fields.

It is a valuable resource for graduate students and researchers on bioinformatics, systems biology, and members of the biomedical field and biotechnology industry who are interested in learning more about CRISP-Cas system and its applications.

Cover image
Title page
Table of Contents
Copyright
List of contributors
Section 1: CRISPR-Cas fundamentals and advances
Chapter 1. CRISPR-Cas9: chronology and evolution
Abstract
1.1 Introduction
1.2 Discovery of CRISPR-Cas system
1.3 Mechanism of CRISPR-Cas system
1.4 Classification of CRISPR-Cas system
1.5 Limitations of CRISPR technology
1.6 Applications of CRISPR technology
1.7 Conclusions and future perspectives
References
Chapter 2. CRISPR: the Janus god of modern science
Abstract
2.1 Introduction
2.2 Timeline of developments in CRISPR-Cas system
2.3 Applications of CRISPR-Cas gene-editing system in human diseases
2.4 Applications of CRISPR system in agriculture for crop improvement
2.5 Applications of CRISPR-Cas9 system in environmental science
2.6 Limitations of CRISPR-Cas9 gene-editing system in medicinal, agricultural, and environmental sectors
2.7 Conclusions
References
Chapter 3. Commercialization of CRISPR-Cas technology: issues and impact
Abstract
3.1 Introduction
3.2 CRISPR-Cas IPR dispute: who stands where?
3.3 Worldwide patent landscape of CRISPR
3.4 A commercial conundrum
3.5 Ethical concerns
3.6 Challenges in commercialization of CRISPR-Cas
3.7 Conclusions
References
Section 2: CRISPR-Cas in medical biotechnology
Chapter 4. CRISPR-Cas-led advancements in translational biotechnology
Abstract
4.1 Introduction
4.2 Genome editing tools before CRISPR
4.3 CRISPR technology
4.4 Knock-outs and knock-ins using CRISPR system
4.5 Applicability of CRISPR beyond just simple editing
4.6 Conclusions and future perspectives
References
Chapter 5. Molecular engineering of CRISPR-Cas system toward in vitro diagnostics
Abstract
5.1 Introduction
5.2 Historical developments
5.3 CRISPR-Cas-mediated sensing of nucleic acid targets
5.4 CRISPR-Cas-mediated sensing of nonnucleic-acid targets
5.5 Challenges
5.6 Conclusions
References
Chapter 6. The CRISPR-Cas technology: trends in healthcare
Abstract
6.1 Introduction
6.2 Overview of the CRISPR-Cas system
6.3 Historical Background
6.4 Emerging applications of CRISPR-Cas systems in healthcare
6.5 Potential of CRISPR-based therapeutics in various diseases and disorders
6.6 CRISPR-Cas9 for developing disease models
6.7 Drug target discovery
6.8 CRISPR babies: ethical or unethical
6.9 Conclusions
References
Chapter 7. CRISPR-Cas system and its role in the development of viral disease diagnostics
Abstract
7.1 Introduction
7.2 CRISPR-Cas in a snapshot
7.3 CRISPR-Cas-based diagnostics
7.4 Conclusions
References
Chapter 8. Unlocking new ways to tackle tuberculosis using CRISPR-Cas as a potent weapon
Abstract
8.1 Introduction to tuberculosis
8.2 Essentiality of tools for tackling tuberculosis
8.3 CRISPR and tuberculosis
8.4 Applications of CRISPR in tackling tuberculosis
8.5 Conclusions and future perspectives
References
Chapter 9. Application of CRISPR-Cas in disease diagnosis and management
Abstract
9.1 Introduction
9.2 Diagnosis of infectious diseases by CRISPR-Cas system
9.3 Diagnosis of noninfectious agents by CRISPR-Cas system
9.4 CRISPR-Cas system for animal and cell models
9.5 CRISPR-Cas system for diagnosis of plant disease
9.6 Challenges and future perspectives
References
Section 3: CRISPR-Cas in environmental biotechnology
Chapter 10. Environmental metagenomics and CRISPR-Cas
Abstract
10.1 Introduction
10.2 CRISPR in the investigation of different microbiomes
10.3 Anti CRISPRs
10.4 Conclusions and future perspective
References
Chapter 11. CRISPR systems tackling fungal infections
Abstract
11.1 Introduction
11.2 CRISPR in Candida
11.3 CRISPR in Cryptococcus species complex
11.4 CRISPR in Aspergillus fumigatus
11.5 Conclusions
References
Section 4: CRISPR-Cas in food and agriculture biotechnology
Chapter 12. CRISPR-Cas technologies in food security and food revolution
Abstract
12.1 Introduction
12.2 What is CRISPR-Cas—an insight
12.3 Applications and limitations of the CRISPR-Cas system
12.4 CRISPR-Cas in agriculture
12.5 CRISPR-Cas in food processing and technology
12.6 Conclusions
References
Chapter 13. Overview of the genome editing in rice and its implications
Abstract
13.1 Introduction
13.2 Technologies for genome editing in rice
13.3 Safety concerns
13.4 Conventional methods for transgene removal
13.5 Conclusions
Acknowledgments
References
Chapter 14. Biotechnological applications of CRISPR-Cas systems in fungi
Abstract
14.1 Introduction
14.2 An historical overview of CRISPR-Cas technological advancements in fungal research
14.3 CRISPR-Cas systems in fungi of biotechnological importance
14.4 Conclusions
References
Chapter 15. Applications and associated challenges of CRISPR-Cas technology in agriculture
Abstract
15.1 Introduction
15.2 CRISPR-based techniques for plant systems
15.3 Applications of CRISPR-Cas technology in crop improvement
15.4 Applications in breeding technologies
15.5 Other breeding technologies
15.6 Challenges with the application of CRISPR-Cas in agriculture
15.7 Conclusions
References
Index

Swati Joshi

Dr Swati Joshi earned PhD in Microbiology from University of Delhi, India. During her Ph.D., she focused on developing industrial strains of methylotrophic yeast Pichia pastoris. Currently she is working as Assistant Professor and BioCARe Women Scientist at School of Life Sciences, Central University of Gujarat (CUG), Gandhinagar, where she is engaged in working in the interdisciplinary field of industrial Microbiology and Biotechnology, employing next gen platforms. She has published many peer reviewed scientific papers, reviews and book chapters. She has been conferred with Dr M. J. Thirumalachar Young Scientist Award by Mycological Society of India (MSI) and Young Scientist Award by Association of Microbiologists of India (AMI). She is life member of BRSI, MSI, AMI and Indian Lichenological Society (ILS).

Affiliations and expertise

Scientist, ICMR-National Institute of Occupational Health (NIOH), Ahmedabad, Gujarat, India (Previous Affiliation: Assistant Professor, School of Life Sciences, Central University of Gujarat, Gandhinagar, India)

Digvijay Verma

Digvijay Verma has been working in the field of genomics and metagenomics for the last 10 years. He has expertise in heterologous expression of genes from extreme environments into the various Gram-negative and Gram-positive bacterial hosts. His research work on proteins reflects his extensive understanding of industrial biotechnology and extremophilic microorganisms. His laboratory is engaged in exploiting therapeutic bioactive molecules from extreme habitats using metagenomic approaches. He is also in a phase of developing pipelines for robust microbiome analysis. Dr. Verma has also been working on analyzing the oral microbiome from the Indian community and the influence of external risk factors on the overall oral bacterial composition. He is a life member of many scientific organizations like the Association of Microbiologists of India (AMI), Biotech Research Society of India (BRSI), and Indian Science Congress (ISC). He is coauthor of Microorganisms for Sustainable Environment and Health (Elsevier, 2020).

Affiliations and expertise

Department of Environmental Microbiology, School of Earth and Environmental Sciences, Babasaheb Bhimrao Ambedkar University, India

Ravi Kr. Gupta

Ravi Kr. Gupta was awarded with several national level scholarships from CSIR and ICMR during his doctoral study and his PhD thesis was awarded Dr. M. M. Dhar Award. Then he moved to the USA as a postdoctoral fellow to enhance his research skills with the state-of-the art techniques in genes and small non-coding RNA regulation in bacteria. Currently he works as assistant professor of microbiology at Babasaheb Bhimrao Ambedkar University (A Central University). His long-term research goals are to better understand the regulatory mechanism of small non-coding RNAs and their role in human and animal pathogens that may lead to the development of alternative therapeutic approach to treat infections. His laboratory is also working to identify novel antimicrobials from natural sources against gram-positive and gram-negative pathogens.

Affiliations and expertise

Assistant Professor, Department of Environmental Microbiology, School of Earth and Environmental Sciences, Babasaheb Bhimrao Ambedkar University, India

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CRISPR-Cas System in Translational Biotechnology

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Swati Joshi

Digvijay Verma

Ravi Kr. Gupta