Covid-19 Molecular Testing and Clinical Correlates, An Issue of the Clinics in Laboratory Medicine

1st Edition, Volume 42-2 - May 31, 2022
Editor: Sanjat Kanjilal
Language: English
Hardback ISBN:
9 7 8 - 0 - 3 2 3 - 8 4 9 5 2 - 4
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 8 4 9 5 3 - 1

In this issue of Clinics in Laboratory Medicine, guest editors Drs. Christopher P. Holstege and Joshua D. King bring their considerable expertise to the topic of COVID-19 Molecular… Read more

Covid-19 Molecular Testing and Clinical Correlates, An Issue of the Clinics in Laboratory Medicine

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In this issue of Clinics in Laboratory Medicine, guest editors Drs. Christopher P. Holstege and Joshua D. King bring their considerable expertise to the topic of COVID-19 Molecular Testing and Clinical Correlates. Top experts in the field cover key topics such as point-of-care molecular assays for SARS-CoV-2; strategies for scaling up SARS-CoV-2 molecular testing capacity; cycle threshold values from SARS-CoV-2 PCR assays; and more.

Cover image
Title page
Table of Contents
Copyright
Contributors
Forthcoming Issues
Preface
Analytic and Clinical Performance of Major Commercial Severe Acute Respiratory Syndrome Coronavirus 2 Molecular Assays in the United States
Key points
Introduction
Overview of severe acute respiratory syndrome coronavirus 2 molecular assays
Analytical performance
Clinical performance
Discussion
Limitations
Summary
Clinics care points
The Successes and Challenges of SARS-CoV-2 Molecular Testing in the United States
Key points
Introduction
Successes
Challenges and trials
Summary
An Overview of SARS-CoV-2 Molecular Diagnostics in Europe
Key points
Introduction
Classification of severe acute respiratory syndrome coronavirus-2, virion, and genome
History of severe acute respiratory syndrome coronavirus-2 molecular diagnostics
Diagnostic testing regulations in Europe
Severe acute respiratory syndrome coronavirus-2 molecular diagnostics
Rapid molecular diagnostics
Stand-alone real-time polymerase chain reaction kits
Low-throughput testing platforms
High-throughput testing platforms
Severe acute respiratory syndrome coronavirus-2 genotyping
Summary
Clinics care points
SARS-CoV-2 Molecular Diagnostics in China
Key points
Introduction
Reverse transcription––polymerase chain reaction
High-throughput sequencing
Isothermal Amplification Assays
CRISPR-based newly developed methods
Antibody detection assays
Antigen-based assays for SARS-CoV-2
Summary
Clinics care points
Rapid Antigen Assays for SARS-CoV-2: Promise and Peril
Key points
Introduction
Background
“Can I have ‘What is the reference method?’ for $800”?
Diagnostic performance in symptomatic populations
Performance as a test of infectiousness in asymptomatic populations
Asymptomatic antigen testing in practice
Conclusions and future considerations
Clinical Diagnostic Point-of-Care Molecular Assays for SARS-CoV-2
Key points
Introduction
Discussion
Clinics care points
Disclosure
Cycle Threshold Values from Severe Acute Respiratory Syndrome Coronavirus-2 Reverse Transcription-Polymerase Chain Reaction Assays: Interpretation and Potential Use Cases
Key points
Introduction
Clinics care points
Performance of Non-nasopharyngeal Sample Types for Molecular Detection of SARS-CoV-2
Key points
Introduction
Respiratory tract specimens
Nonrespiratory specimens
Summary
Clinics care points
Strategies for Scaling up SARS-CoV-2 Molecular Testing Capacity
Key points
Introduction
Pooling
Need for scale-up of molecular assays for SARS-CoV-2
Strategies to consider for large-scale testing for SARS-CoV-2
Limitations of Molecular Assays and Future Strategies for Large-Scale Testing
Summary
Clinics Care Points
Disclosure
Approaches to Deployment of Molecular Testing for SARS-CoV-2 in Resource-Limited Settings
Key points
Introduction
Systems strengthening
Role of the national reference laboratory
Polymerase chain reaction platforms
Impact on tuberculosis and human immunodeficiency virus control programs
Transition to antigen tests and use cases for molecular tests
Approaches to cost reduction
Concluding remarks and future directions
Clinics care points
Disclosure
Novel Assays for Molecular Detection of Severe Acute Respiratory Syndrome Coronavirus 2
Key points
Introduction
Efficiency enhancing adaptations for molecular testing
Variant targeting polymerase chain reaction
Reverse transcription–polymerase chain reaction assays targeting replication intermediates
Next-generation sequencing
Clustered regularly interspaced short palindromic repeats-associated–based diagnostic testing for severe acute respiratory syndrome coronavirus 2
Microfluidic assays for severe acute respiratory syndrome coronavirus 2
Disclosure

Life sciences

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Health

Covid-19 Molecular Testing and Clinical Correlates, An Issue of the Clinics in Laboratory Medicine

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Sanjat Kanjilal