Covid-19: Biomedical Perspectives

1st Edition, Volume 50 - May 24, 2022
Editors: Charles S. Pavia, Volker Gurtler
Language: English
Hardback ISBN:
9 7 8 - 0 - 3 2 3 - 8 5 0 6 1 - 2
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 8 5 3 4 0 - 8

Covid-19: Biomedical Perspectives, Volume 50 in the Methods in Microbiology series highlights new advances in the field, with this new volume presenting interesting chapters writ… Read more

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Covid-19: Biomedical Perspectives, Volume 50 in the Methods in Microbiology series highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Individual chapters in this new release include Sensitive methods for detection of SARS-CoV-2 RNA, Treatment of COVID-19 using Chinese herbal medicine, Understanding how SARS-CoV-2 is evolving and its impact on COVID-19 animal models and vaccine evaluation, Methods in machine learning to identify COVID-19 literature, COVID-19 seasonal behavior and the mutational landscape of the SARS-CoV-2 virus, CRISPR use in Diagnosis and Therapy for COVID-19, and much more.

Cover
Title page
Table of Contents
Copyright
Dedication
Contributors
Preface
Chapter 1: Sensitive methods for detection of SARS-CoV-2 RNA
Abstract
1: Introduction
2: General approaches for the detection of SARS-CoV-2
3: Isothermal amplification methods for sensitive detection of SARS-CoV-2
4: Sensitive detection of SARS-CoV-2 via RT-RPA
5: General considerations for designing an ultrasensitive RT-RPA assay
6: Comparative reviews of recently published RT-RPA assays for SARS-CoV-2 detection
7: Methods section
8: Before you begin
9: Key resources table
10: Materials and equipment
11: Step-by-step method details
12: Summary
Acknowledgement
References
Chapter 2: The seasonal behaviour of COVID-19 and its galectin-like culprit of the viral spike
Abstract
1: Introduction
2: The seasonal behaviour of viruses
3: The seasonal behaviour of COVID-19
4: Viral genomic make up and seasonality
5: Conclusions and prospects
References
Further reading
Chapter 3: Current molecular diagnostics assays for SARS-CoV-2 and emerging variants
Abstract
1: Introduction
2: SARS-CoV-2 variants of concern
3: COVID-19 diagnostics
4: Diagnostics in the era of COVID-19 vaccination
5: Conclusion
References
Further reading
Chapter 4: CRISPR use in diagnosis and therapy for COVID-19
Abstract
1: Introduction
2: Diagnostics and therapeutics for SARS-CoV-2
3: CRISPR/Cas systems
4: CRISPR-based therapeutics for SARS-Cov-2
5: Delivery of CRISPR/Cas components
6: Limitations of CRISPR/Cas system
7: Summary
References
Chapter 5: Recent and advanced nano-technological strategies for COVID-19 vaccine development
Abstract
1: Introduction
2: The structure and infection mechanism of SARS-COV-2
3: Pathogenesis and clinical presentation of COVID-19
4: Vaccine development strategies and platforms
5: Relevant SARS-CoV-2 antigen explored in the design of vaccines
6: Nano-based strategies for COVID-19 vaccine development
7: Benefits and challenges of nanotechnology in COVID-19 vaccine development
8: Conclusion and future perspectives
References
Chapter 6: A review of hypersensitivity methods to detect immune responses to SARS-CoV-2
Abstract
1: Historical perspective
2: General overview, classification and description of hypersensitivity reactions
3: Skin test application of hypersensitivity reactions: In vivo measurements of immune responses
4: In vitro methods to measure immune responses after SARS-Cov-2 infection
5: A novel application of a DTH method to measure immune responses after SARS-CoV-2 infection
6: DTH to measure immunogenicity elicited by covid vaccines
7: Future prospects of DTH to study SARS-CoV-2 immunogenicity
Acknowledgements
References
Chapter 7: Hesitancy to get vaccinated against COVID-19 and how it might be overcome
Abstract
References
Chapter 8: The emergence of SARS-CoV-2 variants of concern in Australia by haplotype coalescence reveals a continental link to COVID-19 seasonality
Abstract
1: Introduction
2: Methods
3: VOCs in Australia
4: Prevalence of amino acid variants in Australia
5: A network view of haplotype diversity and VOC emergence
6: Continental links to seasonality
7: Discussion
References
Chapter 9: COVID-19 vaccines for high risk and immunocompromised patients
Abstract
1: Introduction
2: Development of COVID-19 vaccines
3: The use of COVID-19 vaccines for the high-risk patient population with the emphasis on HIV-infected patients
References

Charles S. Pavia

Dr. Pavia is a Microbiologist/Immunologist and an Associate Professor at the NYIT College of Osteopathic Medicine in Old Westbury, New York. He is also an Adjunct Associate Professor at New York Medical College. He previously worked and held research appointments at the Trudeau Institute in Saranac Lake, NY, and the Walter Reed Army Institute for Research in Washington, DC. Throughout his career, he has focused his research activities on various pathogenic microbes with a particular emphasis on the disease-causing spirochetes and certain protozoan pathogens. His research has covered multiple areas including the basic microbiologic, immunologic, molecular, diagnostic and clinical aspects of Lyme disease, syphilis, malaria and toxoplasmosis. He has published widely on these areas in peer-reviewed journals, as well as given numerous research presentations of his work at national and international conferences. As a principal investigator or co-investigator, he has received research funding from the NIH, the CDC, the J.M. Foundation, and various other organizations to support his research work. He has been a member of the editorial board of the Journal of Microbiological Methods for the past 25 years, and has been an ad-hoc reviewer for a wide range of other scientific journals.

Affiliations and expertise

Associate Professor, NYIT College of Osteopathic Medicine, USA

Volker Gurtler

Dr Gurtler is a Molecular biologist with extensive industry experience and expertise in DNA analysis for the identification and typing of organisms. He is known internationally as the author of the original methodology of ribotyping. He is Advisor to Geneious (Bioinformatics software company) and previous head of Molecular Biology at Austin Health Microbiology, currently Adjunct Professor at RMIT University, Editor of Journal of Microbiological Methods, Editor of Heliyon and Series Editor of Methods in Microbiology. Dr Gurtler is currently working in collaboration with Assoc. Prof Danilla Grando on ribosome analysis of E. coli isolates and association with virulence. He visits RMIT regularly to advise on experimental methods and help PhD students use Geneious Bioinformatic software.

Affiliations and expertise

Adjunct Professor, RMIT University, Melbourne, Australia

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

Health