Correlative Microscopy In Biology
Instrumentation and Methods
- 1st Edition - September 28, 1987
- Editor: M.A. (Eric) Hayat
- Language: English
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 1 4 9 8 6 - 0
Correlative Microscopy in Biology: Instrumentation and Methods presents the detailed methodology of biological correlative microscopy, a technology that allows the acquisition of… Read more
Purchase options
Institutional subscription on ScienceDirect
Request a sales quotePreface
1 Biological Specimen Preparation for Correlative Light and Electron Microscopy
Introduction
Methods
Applications of Correlative Light and Electron Microscopy
References
2 Light and Scanning Electron Microscopy in a Combined Instrument
Introduction
Description of the Combined Instrument
Preparation and Staining Method for LM/SEM Specimens
Quantitative Measurements in the LM/SEM
Cathodoluminescence in the LM/SEM
Biomedical Applications
Developments with Another Type of Scanning Electron Microscope
Conclusions
References
3 Confocal Microscopy in Comparison with Electron and Conventional Light Microscopy
Introduction
The CSLM Instrument
The Morphology of the Nucleoid of Escherichia coli
Characterization of Cell Shape
3-D Imaging Techniques with CSLM
Conclusion and Outlook
References
4 Combined Light and Electron Microscopy of Central Nervous System Neurons and Their Afferent and Efferent Synaptic Connections
Introduction
Morphological Techniques: Correlative Light and Electron Microscopy of Golgi-Impregnated Neurons
Immunocytochemical Techniques: Correlative Light and Electron
Microscopy of Neurons Labeled Immunocytochemically for the
Presence of Neuroactive Substances of Their Synthesizing Enzymes
Correlative Light and Electron Microscopy of Neurons Labeled with PHA-L
References
5 Intracellular Staining of Neurons in the Central Nervous System: Combined Physiological, Morphological, and Cytological Analysis of Identified Neurons
Introduction
Methods
Tissue Processing
Data Analysis
Limitations of the Method
Conclusions
References
6 Correlative Microscopy of Catecholamines and Neuropeptides in Adrenal Medulla
Introduction
Vibratome Sectioning and Fluorescence Microscopy
Immunocytochemistry
Possible Modifications
Double-Labeling Methods for Studying Coexistence
Advantages and Limitations of Combined Faglu-Fluorescence and Immunocytochemistry Method
References
7 Sequential Immunocytochemical and Silver Staining of Neuroendocrine Cells in the Same Section
Introduction
Description of Sequential Staining Technique
Comments on Methodology
Applications
References
8 Correlative Light and Electron Microscopic Method for the Visualization of the Same in Vitro Cell Using Radioautography and Serial Sectioning
Introduction
Methodology
Applications
References
9 Correlative Light and Transmission Electron Microscopy of Mitotic Cells in the Nervous System
Introduction
Mitotic Cells in the Embryonic CNS
Mitotic Cells in the CNS during Myelination
Identification of Mitotic Cells in the Mature and Aged CNS
Applications
References
10 Light and Scanning Electron Microscopy of the Same Metaphase Chromosomes
Introduction
Methodology
Applications of Correlative Light Microscopy and SEM
References
11 Correlative Light and Electron Microscopy on the Same Epoxy Section
Introduction
Methodology
Applications
References
12 Correlative Microscopy of Skeletal Muscle and Endometrium
Introduction
Materials and Methods for Skeletal Muscle
Materials and Methods for Endometrium
Discussion of Methodology and Selected Results
References
13 Correlative Microscopy of Isolated Glomeruli
Introduction
Method for Correlative Microscopy
Illustrated Examples
Concluding Remarks
References
14 Correlative Microscopy of Cell Surface and Underlying Structures
Introduction
Techniques Using Light Microscopy for Cytological Features in Combination with Scanning Electron Microscopy for Surface Structures
Techniques Using Backscattered and Secondary Electron Imaging on Cells and Tissues Processed for Scanning Electron Microscopy
Applications and Future Prospects
References
15 Correlative Light, Scanning, and Transmission Electron Microscopy of Deembedded Tissues
Introduction
Methodology
Applications
References
16 Immunoelectron Microscopic Mirror Method: A Correlative Study
Introduction
Methodology
Correlative Light and Electron Microscopy
Discussion
References
17 The Detection of Amyloid Using Correlative Polarizing Light Microscopy and Electron Microscopy
Introduction
Amyloidosis
Methods
Concluding Remarks
References
18 Correlative Microscopy of Human Vitreous Body
Introduction
Method
Conclusion
References
19 A New Method for Bacterial Identification by Studying the Same Section with Light Microscopy and Scanning and Transmission Electron Microscopy
Introduction
Light Microscopy, Scanning and Transmission Electron Microscopy as Separated Single Tool for Detecting Bacteria within Gingival Tissues
Method
Application
References
20 Correlative Light and Scanning Electron Microscopy of Thick Sections and Isolated Cells
Introduction
Operating Principles
Application of Correlative Scanning Electron Microscopy
Stepwise Procedure
Conclusion
References
Index
- No. of pages: 451
- Language: English
- Edition: 1
- Published: September 28, 1987
- Imprint: Academic Press
- eBook ISBN: 9780323149860
MH
M.A. (Eric) Hayat
Dr. Hayat has published chapters in 54 books, and authored 10 of them. Seven major publishers have published his books: Cambridge University Press, Elsevier, Academic Press, McGraw-Hill, Plenum Press, CRC Press, and Van Nostrand Reinhold Company. Dr. Hayat’s books have been published in Japanese. He has also published a large number of articles in scientific journals and established the Electron Microscopy Center at Kean University.
Degree Information: Ph.D., Biology, Indiana University
Courses Taught: Electron Microscopy; Cell Biology; Seminar
Primary Area of Expertise: Electron microscopy, cell biology, cancer, health and disease. His research interests include immunohistochemistry and in situ hybridization of human carcinomas, including brain metastases and various primary cancers (lung, breast, prostate, colorectal, pancreatic, ovarian, gastrointestinal, and liver).
Advice For Students Preparing for Your Class: "Read the books and work very hard."