
Contamination in Tissue Culture
- 1st Edition - January 1, 1973
- Imprint: Academic Press
- Editor: Jorgen Fogh
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 2 6 1 8 5 0 - 5
- Paperback ISBN:9 7 8 - 0 - 1 2 - 4 3 3 3 8 6 - 4
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 1 5 6 9 8 - 1
Contamination in Tissue Culture covers the sources, prevention, detection, and elimination of contamination in tissue culture. Composed of 12 chapters, the book describes the… Read more

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Request a sales quoteContamination in Tissue Culture covers the sources, prevention, detection, and elimination of contamination in tissue culture. Composed of 12 chapters, the book describes the frequency of occurrence of contamination and the many different effects of contamination on cultured cells. After introducing the intraspecies contamination of cell cultures, the book explains a specific type of contamination, such as bacterial, fungal, viral, and parasitic contamination. A chapter in this book describes the reversible and irreversible alterations of cultured FL human amnion cells after experimental mycoplasmal infection. Chapters 9 and 10 examine the occurrence of tissue culture contaminants by electron microscopy and procedures for isolating and identifying viral contaminants. The concluding chapter covers sterility tests of media and solutions for tissue culture and the use of antibiotics. It also summarizes the major developments made as well as future challenges in the field. This book will be helpful to investigators, teachers, students, and technicians within the many disciplines of cell biology, physiology, cytology, virology, immunology, genetics, oncology, molecular biology, biochemistry, and biophysics, in which tissue and cell cultures are used, either as the primary object of research or as tools.
List of Contributors
Preface
Chapter 1. Extrinsic Cell Contamination of Tissue Culture
I. Introduction
II. Interspecies Identification of Cell Cultures
III. Intraspecies Identification of Cell Cultures
IV. Current Status of Cell Contamination
References
Chapter 2. Methods of Prevention of Bacterial, Fungal, and Other Contaminations
I. Source of Infection
II. Procedures within the Transfer Room
III. Laminar Flow Transfer Room
IV. Aseptic Technique
V. Antibiotics
VI. Summary
References
Chapter 3. Contamination of Tissue Culture by Bacteria and Fungi
I. Introduction
II. Materials and Methods
III. Results
IV. Discussion
V. Summary
References
Chapter 4. Contaminants Demonstrated by Microscopy of Living Tissue Cultures or of Fixed and
Stained Tissue Culture Preparations
I. Preparation of Cell Cultures for Microscopy
II. Methods of Microscopy
III. Photomicrographs Illustrating Various Types of Contaminants in Cell Cultures 69
IV. Review of Data Obtained by Microscopy of Mycoplasma-Infected Cultures after Hypotonic Treatment and Orcein Staining
References
Chapter 5. Contamination of Mammalian Cells in Culture by Mycoplasmata
I. Introduction
II. Biology of the Mycoplasmatales
III. Epidemiology of the Contamination of Cell Cultures with Mycoplasmata
IV. Host-Parasite Relationship of Mycoplasmata and Mammalian Cells
V. Effect of Mycoplasmata on Cell Cultures
VI. Elimination of Mycoplasmata from Cell Cultures
VII. Conclusions
References
Chapter 6. Mycoplasmal Contamination of Cell Cultures: Mycoplasma-Virus-Cell Culture Interactions
I. Introduction
II. Classification
III. Detecting Cell Culture Contamination
IV. Mycoplasmal Contamination
V. Interactions between Mycoplasmas and Animal Cells
VI. Mycoplasma-Virus-Cell Interactions
References
Chapter 7. Reversible and Irreversible Alterations of Cultured FL Human Amnion Cells after
Experimental Mycoplasmal Infection
I. Effects of Mycoplasmal Infection on the in Vitro Growth of FL Cells
II. In Vitro Growth and Susceptibility to Mycoplasmal Infection of Previously
Mycoplasma-Infected Cells from which Mycoplasmas Have Been Eliminated
III. Chromosome Changes following Mycoplasmal Infection of FL Cells
IV. Reduced Tumor-Producing Capacity of Mycoplasma-Modified FL Cells
V. Effects of Mycoplasmas on SV40 Transformation of Human Amnion Cells
VI. Decreased Resistance of Transformed Human Cells to the Destructive Effects of Mycoplasmal Infection
VII. Cell-Associated Mycoplasmas: Preferential Attachment to Transformed Cells and Cells of Malignant Origin
VIII. Discussion of Results
References
Chapter 8. Contamination in Tissue Culture by Parasites
I. Introduction
II. Identification of the Amoebae
III. Source of Contamination
IV. Methods of Detection, Prevention, and Cure
V. Summary
References
Chapter 9. Identification of Tissue Culture Contaminants by Electron Microscopy
I. Introduction
II. Examples of Tissue Culture Contaminations Observed under the Electron Microscope
III. Normal Particulate Cell Organelles of Viral Size
IV. Abnormal Cell Structures of Possible Viral Significance
V. Methods of Electron Microscopic Identification of Viral Contaminants
References
Chapter 10. Endogenous Virus Contaminants in Fetal Bovine Serum and Their Role in Tissue
Culture Contamination
I. Introduction
II. Target Viruses
III. Procedures for Isolating and Identifying Viral Contaminants
IV. Results
V. Discussion and Conclusions
References
Chapter 11. The Presence of Viruses in Uninoculated Tissue Cultures: Sources and Methods of
Detection
I. Introduction
II. Passenger, Indigenous, and Cryptic Viruses
III. The Relationship of Viruses to an Ability of Cells to Grow in Culture
IV. Significance of the Presence of Viruses Inherent to Cultured Cells
V. Detection of Viruses Indigenous to Cultured Cells
References
Chapter 12. Summary
I. Introduction
II. Types and Sources of Contamination
III. Sterility Tests of Media and Solutions
IV. Detection and Identification of Contaminants
V. Prevention of Contamination
VI. Treatment of Contaminated Cultures
VII. Use of Antibiotics
VIII. Effects of Contaminants on Cell Cultures
IX. Concluding Remarks
Reference
Author Index
Subject Index
- Edition: 1
- Published: January 1, 1973
- Imprint: Academic Press
- No. of pages: 300
- Language: English
- Hardback ISBN: 9780122618505
- Paperback ISBN: 9780124333864
- eBook ISBN: 9780323156981
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