Conformation in Biology and Drug Design

Contributors

Preface

Nomenclature and Abbreviations

Chapter 1 A Perspective on the Application of Physical Methods to Peptide Conformational-Biological Activity Studies

I Introduction

II Some Biological Considerations

III Historical Development of Peptide Conformational Studies in Solution

IV Theoretical Methods for Examining Peptide Conformations

V Application of Conformational and Dynamic Studies of Peptides to Their Biological Activities

References

Chapter 2 Circular Dichroism of Peptides

I Introduction

II General Aspects of Peptide CD

III Specific Systems

References

Chapter 3 Application of Fluorescence Techniques in Studies of Peptide Conformations and Interactions

I Introductory Remarks

II Fluorophores in Peptides

III Fluorescence Quantum Yield and Lifetime Measurements

IV Fluorescence Polarization Studies

V Measurement of Intramolecular Distances by Singlet-Singlet Resonance Energy Transfer Experiments

VI Fluorescence Studies of Peptide Interactions with Macromolecular Systems

VII Concluding Remarks

References

Chapter 4 Theoretical Methods in the Analysis of Peptide Conformation

I Introduction

II Definitions and Conventions

III Methodology

IV Results

References

Chapter 5 Theoretical Simulation of Conformation, Energetics, and Dynamics of Peptides

I Introduction

II Techniques and Methodology

III Applications

IV Summary and Future Directions

References

Chapter 6 Paramagnetic Metal Ions as Nuclear Magnetic Resonance Probes of Peptide Conformation in Solution

I Introduction

II Paramagnetic Metal Ion-Induced Shifts and Relaxation Enhancements

III Conclusions

References

Chapter 7 Nuclear Magnetic Resonance Studies of Peptide-Macromolecule Interactions

I Introduction

II Nuclear Magnetic Resonance Techniques

III Peptide-Protein Interaction

IV Peptide-Lipid Interaction

V Peptide-Nucleic Acid Interactions

VI Conclusions and Prospects

References

Chapter 8 Solid-State Nuclear Magnetic Resonance of Peptides

I Introduction

II Methods

III Applications

IV Prospects

References

Chapter 9 Modern Nuclear Magnetic Resonance Spectroscopy of Peptides

I Introduction

II Conformational Homogeneity

III General Strategy for Nuclear Magnetic Resonance Investigation of Peptides

IV Assignment of Proton Nuclear Magnetic Resonance Signals

V Heteronuclear Techniques

VI Sequence Analysis

VII Quantitative Evaluation of Nuclear Magnetic Resonance Spectra

VIII Conclusions

References

Index