Chemokines

1st Edition, Volume 570 - February 24, 2016
Editor: Tracy Handel
Language: English
Hardback ISBN:
9 7 8 - 0 - 1 2 - 8 0 2 1 7 1 - 2
eBook ISBN:
9 7 8 - 0 - 1 2 - 8 0 2 1 9 5 - 8

Chemokines, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volu… Read more

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Chemokines, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in chemokines, and includes sections on such topics as chemokine detection using receptors, tracking cellular responses to chemokines, recognition of GAG-bound chemokines, and the production of chemokine receptor complexes for structural and biophysical studies.

Preface
Chapter One: Chemokine Detection Using Receptors Immobilized on an SPR Sensor Surface
- Abstract
- 1 Surface Plasmon Resonance
- 2 Chemokine Receptors: Members of the GPCR Family
- 3 Chemokine Receptor Immobilization on the Sensor Chip
- 4 Viral Particles as Chemokine Receptors Carriers in SPR
- 5 SPR-Based Applications for Chemokine Receptors
- 6 Conclusions
- Acknowledgments
Chapter Two: Study of Chemotaxis and Cell–Cell Interactions in Cancer with Microfluidic Devices
- Abstract
- 1 Introduction
- 2 Methods
- 3 Limitations
- 4 Perspectives
- Acknowledgments
Chapter Three: Generating Chemokine Analogs with Enhanced Pharmacological Properties Using Phage Display
- Abstract
- 1 Introduction
- 2 Methods
- 3 Limitations
- 4 Perspectives
Chapter Four: Methods for the Recognition of GAG-Bound Chemokines
- Abstract
- 1 Introduction
- 2 Methods
- 3 Summary
Chapter Five: Monitoring Scavenging Activity of Chemokine Receptors
- Abstract
- 1 Introduction
- 2 Fluorescent Chemokines
- 3 Monitoring Scavenging with Radiolabeled Chemokines
- 4 Monitoring Scavenging with Fluorescent Chemokines
- 5 Monitor Chemokine Uptake by Microscopy and Flow Cytometry (FACS)
Chapter Six: Dual-Color Luciferase Complementation for Chemokine Receptor Signaling
- Abstract
- 1 Introduction
- 2 Methods
- Acknowledgment
Chapter Seven: Analysis of Arrestin Recruitment to Chemokine Receptors by Bioluminescence Resonance Energy Transfer
- Abstract
- 1 Introduction
- 2 Methods
- 3 Interpretation and Limitations of BRET Data
- 4 Perspectives
- Acknowledgments
Chapter Eight: Probing Biased Signaling in Chemokine Receptors
- Abstract
- 1 Introduction
- 2 Types of Bias in the Chemokine System
- 3 Chemokine System-Mediated Intracellular Signaling
- 4 Methods
- Acknowledgments
Chapter Nine: Mutagenesis by Phage Display
- Abstract
- 1 Introduction
- 2 Investigating the Pharmacophore of Chemokine Binders by Phage Display
- 3 Phage Display Selection to Modulate the Selectivity of Chemokine Binders
- 4 Summary
Chapter Ten: Studying Chemokine Control of Neutrophil Migration In Vivo in a Murine Model of Inflammatory Arthritis
- Abstract
- 1 Introduction
- 2 Methods
- 3 Conclusions
Chapter Eleven: Production of Chemokine/Chemokine Receptor Complexes for Structural Biophysical Studies
- Abstract
- 1 Introduction
- 2 Methods
- 3 Summary and Conclusions
- Acknowledgments
Chapter Twelve: In Vivo Models to Study Chemokine Biology
- Abstract
- 1 Introduction
- 2 Methods
- 3 Limitations
- 4 Other Perspectives
Chapter Thirteen: Monitoring Chemokine Receptor Trafficking by Confocal Immunofluorescence Microscopy
- Abstract
- 1 Introduction
- 2 Methods
- 3 Concluding Remarks
Chapter Fourteen: Active Shaping of Chemokine Gradients by Atypical Chemokine Receptors: A 4D Live-Cell Imaging Migration Assay
- Abstract
- 1 Background
- 2 Introduction
- 3 Materials and Equipment
- 4 Preparation of Chambers
- 5 Preparation of Cells
- 6 Filling of Chambers
- 7 Time-Lapse Imaging
- 8 Analysis
- Acknowledgments
Chapter Fifteen: The Role of Chemokine and Glycosaminoglycan Interaction in Chemokine-Mediated Migration In Vitro and In Vivo
- Abstract
- 1 Introduction
- 2 In Vitro Chemotaxis
- 3 In Vivo Chemotaxis
- 4 Generation of Mammalian Transfectants Expressing Chemokine Receptors
- 5 Discussion
- Acknowledgments
Chapter Sixteen: Examining Roles of Glycans in Chemokine-Mediated Dendritic–Endothelial Cell Interactions
- Abstract
- 1 Introduction
- 2 Methods
- 3 Perspectives
- Acknowledgments
Chapter Seventeen: Preparation and Analysis of N-Terminal Chemokine Receptor Sulfopeptides Using Tyrosylprotein Sulfotransferase Enzymes
- Abstract
- 1 Introduction
- 2 Methods
- 3 Caveats and Limitations
- 4 Perspectives
- Acknowledgments
Chapter Eighteen: Disulfide Trapping for Modeling and Structure Determination of Receptor: Chemokine Complexes
- Abstract
- 1 Introduction
- 2 Architecture of Receptor:Chemokine Interfaces
- 3 Cysteine as a Natural Crosslinking Agent
- 4 Disulfide Trapping
- 5 Conclusion and Perspectives
- Acknowledgments
Chapter Nineteen: Analysis of G Protein and β-Arrestin Activation in Chemokine Receptors Signaling
- Abstract
- 1 Introduction
- 2 G Proteins Signaling
- 3 β-Arrestins Signaling: Detection of β-Arrestins Recruitment by Protein Conformational Changes
- 4 Calculation of Biased Signaling
- 5 Summary
Chapter Twenty: Flow Cytometry Detection of Chemokine Receptors for the Identification of Murine Monocyte and Neutrophil Subsets
- Abstract
- 1 Introduction
- 2 Blood Collection and Preparation of Blood Cells from Mice
- 3 Staining of Cell Surface Markers and Chemokine Receptors
- 4 Flow Cytometry Analysis
- 5 Discussion
- 6 Concluding Remarks
- Acknowledgments
Chapter Twenty-One: Molecular Pharmacology of Chemokine Receptors
- Abstract
- 1 Introduction
- 2 Pharmacological Quantification of Chemokine Receptor Binding
- 3 Pharmacological Quantification of Chemokine Receptor Signaling
- 4 Conclusions and Future Perspectives
- Acknowledgments
Chapter Twenty-Two: Preparation and Characterization of Glycosaminoglycan Chemokine Coreceptors
- Abstract
- 1 Introduction
- 2 Preparation and Characterization of GAGs
- 3 Methods for Studying Chemokine–GAG Interactions
- 4 Concluding Remarks
- Acknowledgment
Chapter Twenty-Three: Production of Recombinant Chemokines and Validation of Refolding
- Abstract
- 1 Introduction
- 2 Methods
- 3 Caveats and Limitations
- 4 Perspectives
- Acknowledgments
Chapter Twenty-Four: Quantitative Analysis of Dendritic Cell Haptotaxis
- Abstract
- 1 Introduction
- 2 Methods
- 3 Perspectives
- Acknowledgments
Author Index
Subject Index

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

Health

Chemokines

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Tracy Handel