Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods
- 1st Edition, Volume 641 - July 23, 2020
- Editor: David M. Chenoweth
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 8 2 1 1 5 6 - 4
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 2 1 1 5 5 - 7
Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods, Volume 641 in the Methods in Enzymology series, c… Read more
Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods, Volume 641 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include caged cyclopropanes with improved tetrazine ligation kinetics, an analysis of metabolically labeled inositol phosphate messengers by NMR, cell-permeant caged inositol pyrophosphates for probing β-cells, imaging phospholipase D activity with clickable alcohols via transphosphatidylation, fluorescent biorthogonal labeling of class B GPCRs in live cells, near-infrared photoactivatable nitric oxide donors with integrated photoacoustic monitoring, and much more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Enzymology series
- Includes the latest information on retinoid signaling pathways
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
- Enzyme- or light-triggered cyclopropenes for bioorthogonal ligation
- Analysis of metabolically labeled inositol phosphate messengers by NMR
- Photo-releasable derivatives of inositol pyrophosphates
- IMPACT: Imaging phospholipase D activity with clickable alcohols via transphosphatidylation
- Fluorescent bioorthogonal labeling of class B GPCRs in live cells
- Near-infrared photoactivatable nitric oxide donors with photoacoustic readout
- H2S donors with optical responses
- Visualization of oxidative stress-induced carbonylation in live mammalian cells
- Receptor-based fluorescent sensors constructed from ribonucleopeptide
- Conformationally restrained pentamethine cyanines and use in reductive single molecule localization microscopy
- A near-infrared light-mediated cleavable linker strategy using the heptamethine cyanine chromophore
- Quantitative measurement of cytosolic penetration using the chloroalkane penetration assay
- Cell-penetrating and mitochondrion-targeting molecules
- A photocaged DNA nanocapsule for delivery and manipulation in cells
- A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells
- Enzymatic covalent labeling of RNA with RNA transglycosylation at guanosine (RNA-TAG)
- Assaying RNA solvent accessibility in living cells with LASER
- Split T7 RNA polymerase biosensors to study multiprotein interaction dynamics
- Cross-linking cellular nucleic acids via a target-directing double click reagent
- Click chemistry-based amplification and detection of endogenous RNA and DNA molecules in situ using clampFISH probes
- Quantification of protein delivery in live cells using fluorescence correlation spectroscopy
Ting Jiang and Scott T. Laughlin
Robert Puschmann, Robert K. Harmel and Dorothea Fiedler
Sebastian Hauke, Tamara Bittner, Henning J. Jessen and Carsten Schultz
Timothy W. Bumpus, Dongjun Liang and Jeremy M. Baskin
Srikanth Kumar Gangam and Qing Lin
Effie Y. Zhou, Hailey J. Knox, Christopher J. Reinhardt, Gina Partipilo and Jefferson Chan
Michael D. Pluth, Yu Zhao and Matthew M. Cerda
Kamalika Mukherjee, Tak Ian Chio and Susan L. Bane
Skun Nakano, Hiroaki Konishi and Takashi Morii
Siddharth S. Matikonda, Ralph Götz, Ryan McLaughlin, Markus Sauer and Martin J. Schnermann
Michael P. Luciano, Saghar Nourian, Alexander P. Gorka, Roger R. Nani, Tadanobu Nagaya, Hisataka Kobayashi and Martin J. Schnermann
Kirsten Deprey and Joshua A. Kritzer
George Appiah Kubi and Dehua Pei
Yihong Feng, Takeshi Tohgasaki, Yasuyuki Shitomi, Hiroshi Sugiyama and Masayuki Endo
Esther Braselmann and Amy E. Palmer
Kayla N. Busby and Neal K. Devaraj
Chao Feng and Robert C. Spitale
Jeffrey A. Dewey and Bryan C. Dickinson
Masayuki Tera and Nathan W. Luedtke
Sepideh Tavakoli, Yifang Liu, Jacob Potts and Sara H. Rouhanifard
Susan L. Knox, Angela Steinauer, Garrett Alpha-Cobb, Adam Trexler, Elizabeth Rhoades and Alanna Schepartz
- Edition: 1
- Volume: 641
- Published: July 23, 2020
- Language: English
DC
David M. Chenoweth
David Chenoweth is at University of Pennsylvania, PA, USA
Affiliations and expertise
University of Pennsylvania, PA, USARead Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods on ScienceDirect