Cellulases

1. Measurement of endo-1,4-b-glucanase

Barry V. McCleary, Vincent McKie and Anna Draga

2. Biomass Conversion determined via Fluorescent Cellulose Decay assay

Bente Wischmann, Marianne Toft, Marco Malten and KC McFarland

3. The analysis of saccharification in biomass using an automated high-throughput method

Caragh Whitehead, Leonardo D. Gomez, Simon J. McQueen-Mason

4. Studies of enzymatic cleavage of cellulose using Polysaccharide Analysis by Carbohydrate gel Electrophoresis (PACE)

Ondrej Kosik, Jennifer Bromley, Marta Busse-Wicher, Zhinong Zhang & Paul Dupree

5. Measuring processivity

Svein J. Horn, Morten Sørlie, Kjell M. Vårum, Priit Väljamäe and Vincent G.H. Eijsink

6. Distinguishing xyloglucanase activity in endo-b (1® 4) glucanases

Jens M. Eklöf, Marcus C. Ruda and Harry Brumer

7. Methods for structural characterization of the products of cellulose- and xyloglucan-hydrolyzing enzymes

Maria J. Peña, Sami T. Tuomivaara, Breeanna R. Urbanowicz, Malcolm A. O’Neill and William S. York

8. The crystallization and structural analysis of cellulases (and other glycoside hydrolases): strategies and tactics

Shirley M. Roberts and Gideon J. Davies

9. Visualization of Cellobiohydrolase I from Trichoderma reesei Moving on Crystalline Cellulose Using High-Speed Atomic Force Microscopy

Kiyohiko Igarashi, Takayuki Uchihashi, Anu Koivula, Masahisa Wada, Satoshi Kimura, Merja Penttilä, Toshio Ando and Masahiro Samejima


10. Small angle X-ray scattering and crystallography; a winning combination for exploring the multi-modular organisation of cellulolytic macromolecular complexes

Mirjam Czjzek, Henri-Pierre Fierobe and Véronique Receveur-Bréchot

11. Quantitative approaches to the analysis of carbohydrate-binding module function

D. Wade Abbott‡ and Alisdair B. Boraston

12. In Situ Detection Of Cellulose With Carbohydrate-Binding Modules

J. Paul Knox

13. Interactions between family 3 carbohydrate binding modules (CBMs) and cellulosomal linker peptides

Oren Yaniv. Felix Frolow. Maly Levy-Assraf, Raphael Lamed and Edward A. Bayer

14. Approaches for improving thermostability characteristics in cellulases

Michael Anbar and Edward A. Bayer

15. Thermophilic glycosynthases for oligosaccharides synthesis

Beatrice Cobucci-Ponzano, Giuseppe Perugino, Andrea Strazzulli, Mosè Rossi and Marco Moracci


16. Engineering cellulase activity into Clostridium acetobutylicum

Henri-Pierre Fierobe, Florence Mingardon and Angélique Chanal

17. Transformation of Clostridium thermocellum by electroporation

Daniel G. Olson and Lee R. Lynd

18. Genetic and Functional Genomic Approaches for the Study of Plant Cell Wall Degradation in Cellvibrio japonicus

Jeffrey G. Gardner and David H. Keating

19. Methods for the isolation of cellulose-degrading microorganisms

James E. McDonald, David J. Rooks and Alan J. McCarthy

20. Metagenomic approaches to the discovery of cellulases

David J. Rooks, James E. McDonald and Alan J. McCarthy

21. Escherichia coli expression, purification, crystallization and structure determination of bacterial cohesin-dockerin complexes

Joana Brás, Ana Luisa Carvalho, Aldino Viegas, Shabir Najmudin, Victor D. Alves, José A.M. Prates, Luís M.A. Ferreira, Maria J. Romão, Harry J. Gilbert and Carlos M.G.A. Fontes

22. Measurements of relative binding of cohesin and dockerin mutants using an advanced ELISA technique for high-affinity interactions

Michal Slutzki, Yoav Barak, Dan Reshef, Ora Schueler-Furman, Raphael Lamed and Edward A. Bayer

23. Designer cellulosomes for enhanced hydrolysis of cellulosic substrates

Yael Vazana, Sarah Moraïs, Yoav Barak, Raphael Lamed and Edward A. Bayer

24. High-throughput screening of cohesin mutant libraries on cellulose microarrays

Michal Slutzki, Vered Ruimy, Ely Morag, Yoav Barak, Rachel Haimovitz, Raphael Lamed and Edward A. Bayer