Cellular Senescence and Aging
- 1st Edition, Volume 181 - January 12, 2024
- Imprint: Academic Press
- Editors: Giulia Petroni, Oliver Kepp, Lorenzo Galluzzi
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 3 - 1 8 8 9 6 - 1
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 1 8 8 9 7 - 8
Cellular Senescence and Aging, Volume 181 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on t… Read more
Purchase options
Institutional subscription on ScienceDirect
Request a sales quoteCellular Senescence and Aging, Volume 181 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as assessing polyglutamine tract aggregation in the nematode Caenorhabditis elegans, Generation of glial cell-derived neurotrophic factor (gdnf) morphants in zebrafish larvae by cerebroventricular microinjection of vivo morpholino, Methods for detection of mitochondrial reactive oxygen species in senescent cells, Assessment of cell cycle progression and mitotic slippage by videomicroscopy, The original colorimetric method to detect cellular senescence, and more.
Additional sections cover Assessing microbiota composition in the context of aging, Assessing chronological aging in Saccharomyces cerevisiae, Image processing and supervised machine learning for retinal microglia characterization in senescence, Measuring telomerase activity using TRAP assays, High throughput assessment of cellular senescence, Detection of radiation-induced senescence by the Debacq-Chainiaux protocol: Improvements and upgrade in the detection of positive events, Dynamic and scalable assessment of the senescence-associated secretory phenotype (SASP), Flow cytometry-assisted quantification of cell cycle arrest in cancer cells treated with CDK4/6 inhibitors, and more.
Additional sections cover Assessing microbiota composition in the context of aging, Assessing chronological aging in Saccharomyces cerevisiae, Image processing and supervised machine learning for retinal microglia characterization in senescence, Measuring telomerase activity using TRAP assays, High throughput assessment of cellular senescence, Detection of radiation-induced senescence by the Debacq-Chainiaux protocol: Improvements and upgrade in the detection of positive events, Dynamic and scalable assessment of the senescence-associated secretory phenotype (SASP), Flow cytometry-assisted quantification of cell cycle arrest in cancer cells treated with CDK4/6 inhibitors, and more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Cell Biology series
- Updated release includes the latest information on Cellular Senescence and Aging
Researchers and students
- Cover image
- Title page
- Table of Contents
- Series Page
- Copyright
- Contributors
- Cellular senescence and aging at the crossroad between immunity and cancer
- Acknowledgments
- Conflicts of interest
- References
- Chapter 1 Assessing polyglutamine tract aggregation in the nematode Caenorhabditis elegans
- Abstract
- 1 Introduction
- 2 Materials
- 3 Methods
- 4 Concluding remarks
- 5 Notes
- Acknowledgments
- Author contributions
- Conflicts of interest
- References
- Chapter 2 Generation of glial cell-derived neurotrophic factor (gdnf) morphants in zebrafish larvae by cerebroventricular microinjection of vivo morpholino
- Abstract
- 1 Introduction
- 2 Materials
- 3 Optimizing cerebroventricular microinjection of zebrafish larvae
- 4 Cryosectioning and DAPI counterstaining
- 5 Cerebroventricular microinjection for knockdown
- 6 Concluding remarks
- 7 Notes
- References
- Chapter 3 Methods for detection of mitochondrial reactive oxygen species in senescent cells
- Abstract
- 1 Introduction
- 2 Methods for ROS detection in live cells
- 3 Materials and methods
- 4 Results and concluding remarks
- Acknowledgment
- References
- Chapter 4 Assessment of cell cycle progression and mitotic slippage by videomicroscopy
- Abstract
- 1 Introduction
- 2 Materials
- 3 Methods
- 4 Notes
- 5 Concluding remarks
- Acknowledgments
- Disclosures
- References
- Chapter 5 The original colorimetric method to detect cellular senescence
- Abstract
- 1 Introduction
- 2 Materials
- 3 Methods
- 4 SA-β-Gal staining solution
- 5 EdU staining solution
- 6 SA-β-Gal staining of cells
- 7 SA-β-Gal staining of tissue samples
- 8 EdU co-staining
- 9 Example
- 10 Concluding remarks
- 11 Notes
- Acknowledgments
- References
- Chapter 6 Assessing microbiota composition in the context of aging
- Abstract
- 1 Introduction
- 2 Materials
- 3 Methods
- 4 Notes
- 5 Concluding remarks
- Acknowledgments
- References
- Chapter 7 Assessing chronological aging in Saccharomyces cerevisiae
- Abstract
- 1 Introduction
- 2 General aspects
- 3 CLS determination
- 4 Cell death characterization
- 5 High-throughput screening
- 6 Notes
- 7 Conclusion
- References
- Chapter 8 Image processing and supervised machine learning for retinal microglia characterization in senescence
- Abstract
- 1 Introduction
- 2 Retinal dissection
- 3 Immunostaining and mounting
- 4 Imaging and image processing
- 5 SML SVM development for microglia characterization
- 6 Perivascular microglia quantification
- 7 Data analysis
- 8 Concluding remarks
- Author Contributions
- Acknowledgments
- References
- Chapter 9 Measuring telomerase activity using TRAP assays
- Abstract
- 1 Telomerase and its effect onto telomeres
- 2 Telomerase activity in somatic, stem-and cancer cells
- 3 TRAP as a method for measuring telomerase activity
- 4 Other non-gel-based methods
- 5 Quantification of TA and interpretation of results from conventional TRAPs
- 6 Novel TRAP methods using isothermal amplification and CRISPR-Cas technology
- 7 Conclusions
- References
- Chapter 10 High-throughput assessment of cellular senescence
- Abstract
- 1 Introduction
- 2 Material
- 3 Methods
- 4 Concluding remarks
- 5 Notes
- Acknowledgments
- Conflicts of interest
- Data availability statement
- References
- Chapter 11 Detection of radiation-induced senescence by the Debacq-Chainiaux protocol: Improvements and upgrade in the detection of positive events
- Abstract
- 1 Introduction
- 2 Equipment
- 3 Reagents
- 4 Irradiation on LINAC
- 5 HUVECs cell culture
- 6 Optimization of instrument settings, gating strategy and data acquisition
- 7 Evaluation of senescence (C12FDG) using spectral flow cytometry
- 8 Data analysis
- 9 Overview of the protocol and general considerations
- 10 Notes
- Acknowledgments
- Author contributions
- Competing interests
- References
- Chapter 12 Dynamic and scalable assessment of the senescence-associated secretory phenotype (SASP)
- Abstract
- 1 Introduction
- 2 Materials
- 3 Methods
- 4 Notes
- Acknowledgments
- References
- Chapter 13 Flow cytometry-assisted quantification of cell cycle arrest in cancer cells treated with CDK4/6 inhibitors
- Abstract
- 1 Introduction
- 2 Materials
- 3 Methods
- 4 Notes
- 5. Concluding remarks
- Acknowledgment
- Competing interests
- References
- Chapter 14 Mechanistic exploration of autophagy and aging by RNA interference
- Abstract
- 1 Introduction
- 2 Materials
- 3 Concluding remarks
- 4 Notes
- Acknowledgments
- Conflicts of interest
- Data availability statement
- References
- Edition: 1
- Volume: 181
- Published: January 12, 2024
- No. of pages (Hardback): 250
- No. of pages (eBook): 318
- Imprint: Academic Press
- Language: English
- Hardback ISBN: 9780443188961
- eBook ISBN: 9780443188978
GP
Giulia Petroni
Giulia Petroni is a PhD Fellow at the Department of Experimental and Clinical Medicine, University of Florence. Giulia does research in Oncology, focusing on the ion channels' role in Colorectal Cancer. She has also an expertise in Clinical Immunology and Immunogenicity. She has published over 15 articles in international peer-reviewed journals.
Affiliations and expertise
Department of Experimental and Clinical Medicine, University of Florence, Florence, ItalyOK
Oliver Kepp
Dr. Oliver Kepp graduated from Humboldt University Berlin and obtained a PhD degree in Molecular Biology. Today he is a tenured researcher at INSERM and a Co-director of the BioCell automation platform at GRCC. Oliver and his team investigate several aspects of tumor immunogenicity, focusing on systems cell biology approaches. He is also a co-founder of Samsara therapeutics and was appointed associate director of the European academy of tumor immunology in 2019. Oliver received the prestigious Clarivate Highly Cited Researcher Award four years in a row since 2018 and has recently been ranked among the top biology and biochemistry scientists in France (#5; www.research.com)
Affiliations and expertise
Equipe labellisée par la Ligue contre le cancer, Université de Paris, Sorbonne UniversitéLG
Lorenzo Galluzzi
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.
Affiliations and expertise
Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USARead Cellular Senescence and Aging on ScienceDirect