Cell Polarity and Morphogenesis

Series Editors

Chapter 1. A user's guide for characterizing plasma membrane subdomains in living cells by spot variation fluorescence correlation spectroscopy

• Introduction
• 1. Building a Sensitive and Robust Homemade Spot Variation Fluorescence Correlation Spectroscopy/Fluorescence Recovery After Photobleaching Setup
• 2. Sample Preparations and Labeling
• 3. Fluorescence Correlation Spectroscopy Recording
• 4. Data Analysis and Interpretation
• Conclusion

Chapter 2. Investigating symmetry breaking in yeast: From seeing to understanding

• Introduction
• 1. Genetic Tools
• 2. Microscopy-Based Methods to Visualize Cell Polarization
• Conclusion and Future Perspective
• Notes

Chapter 3. Precise tracking of the dynamics of multiple proteins in endocytic events

• Introduction
• 1. Rationale
• 2. Sample Preparation
• 3. Fluorescence Microscopy
• 4. Image Processing
• 5. Tracking of the Endocytic Spots
• 6. Correction of Dual Channel Trajectory Pair Alignment
• 7. Generation of Average Trajectories and Alignment of the Average Trajectories
• Conclusion

Chapter 4. Quantitative approaches for the study of microtubule aster motion in large eggs

• Introduction
• 1. Three-Dimensional Tracking of Microtubule Asters in Sea Urchin Embryos
• 2. Laser Ablation of Astral Microtubules and In Situ Immunostaining
• Conclusions

Chapter 5. Dissection and characterization of microtubule bundles in the mitotic spindle using femtosecond laser ablation

• Introduction
• 1. Sample Preparation
• 2. Imaging
• 3. Laser Ablation
• 4. Image Analysis
• Conclusion

Chapter 6. Fluorescence eXclusion Measurement of volume in live cells

• Introduction
• 1. Method
• 2. FXm Pitfalls and Limitations
• 3. Discussion

Chapter 7. Quantitative methods to study epithelial morphogenesis and polarity

• Introduction
• 1. Biological Toolbox
• 2. Imaging Toolbox
• 3. Image Processing Toolbox
• 4. Measurements
• Concluding Remarks

Chapter 8. Probing tissue interaction with laser-based cauterization in the early developing Drosophila embryo

• Introduction
• 1. Materials
• 2. Methods
• Conclusion

Chapter 9. Optogenetic inhibition of apical constriction during Drosophila embryonic development

• Introduction
• 1. Methods
• Conclusion

Chapter 10. Micropipette aspiration: A unique tool for exploring cell and tissue mechanics in vivo

• Introduction
• 1. Building and Preparing a Microaspiration Setup
• 2. Subcellular Surface Tension Mapping
• 3. Mechanical Properties of Tissues
• General Conclusions
• Supplementary Data

Chapter 11. In-vivo analysis of morphogenesis in plants

• Introduction
• 1. Measuring the Growth: Live Imaging of Growing Tissues at Cellular Resolution
• 2. Probing the Wall Stiffness and Anisotropy
• Conclusions and Perspectives: Beyond Quantitative Live Imaging
• Acknowledgments