Cell Polarity and Morphogenesis

1st Edition, Volume 139 - February 20, 2017
Editor: Thomas Lecuit
Language: English
Hardback ISBN:
9 7 8 - 0 - 1 2 - 8 0 9 3 7 3 - 3
eBook ISBN:
9 7 8 - 0 - 1 2 - 8 0 9 4 5 7 - 0

Cell Polarity and Morphogenesis, the latest volume in the Methods in Cell Biology series, looks at cell polarity and morphogenesis. Edited by leaders in the field, this volume prov… Read more

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Cell Polarity and Morphogenesis, the latest volume in the Methods in Cell Biology series, looks at cell polarity and morphogenesis. Edited by leaders in the field, this volume provides proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies.

Series Editors

Chapter 1. A user's guide for characterizing plasma membrane subdomains in living cells by spot variation fluorescence correlation spectroscopy

Introduction
1. Building a Sensitive and Robust Homemade Spot Variation Fluorescence Correlation Spectroscopy/Fluorescence Recovery After Photobleaching Setup
2. Sample Preparations and Labeling
3. Fluorescence Correlation Spectroscopy Recording
4. Data Analysis and Interpretation
Conclusion

Chapter 2. Investigating symmetry breaking in yeast: From seeing to understanding

Introduction
1. Genetic Tools
2. Microscopy-Based Methods to Visualize Cell Polarization
Conclusion and Future Perspective
Notes

Chapter 3. Precise tracking of the dynamics of multiple proteins in endocytic events

Introduction
1. Rationale
2. Sample Preparation
3. Fluorescence Microscopy
4. Image Processing
5. Tracking of the Endocytic Spots
6. Correction of Dual Channel Trajectory Pair Alignment
7. Generation of Average Trajectories and Alignment of the Average Trajectories
Conclusion

Chapter 4. Quantitative approaches for the study of microtubule aster motion in large eggs

Introduction
1. Three-Dimensional Tracking of Microtubule Asters in Sea Urchin Embryos
2. Laser Ablation of Astral Microtubules and In Situ Immunostaining
Conclusions

Chapter 5. Dissection and characterization of microtubule bundles in the mitotic spindle using femtosecond laser ablation

Introduction
1. Sample Preparation
2. Imaging
3. Laser Ablation
4. Image Analysis
Conclusion

Chapter 6. Fluorescence eXclusion Measurement of volume in live cells

Introduction
1. Method
2. FXm Pitfalls and Limitations
3. Discussion

Chapter 7. Quantitative methods to study epithelial morphogenesis and polarity

Introduction
1. Biological Toolbox
2. Imaging Toolbox
3. Image Processing Toolbox
4. Measurements
Concluding Remarks

Chapter 8. Probing tissue interaction with laser-based cauterization in the early developing Drosophila embryo

Introduction
1. Materials
2. Methods
Conclusion

Chapter 9. Optogenetic inhibition of apical constriction during Drosophila embryonic development

Introduction
1. Methods
Conclusion

Chapter 10. Micropipette aspiration: A unique tool for exploring cell and tissue mechanics in vivo

Introduction
1. Building and Preparing a Microaspiration Setup
2. Subcellular Surface Tension Mapping
3. Mechanical Properties of Tissues
General Conclusions
Supplementary Data

Chapter 11. In-vivo analysis of morphogenesis in plants

Introduction
1. Measuring the Growth: Live Imaging of Growing Tissues at Cellular Resolution
2. Probing the Wall Stiffness and Anisotropy
Conclusions and Perspectives: Beyond Quantitative Live Imaging
Acknowledgments

Life Sciences

Physical Sciences & Engineering

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Health

Cell Polarity and Morphogenesis

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