Cell Physiology Source Book
- 5th Edition - November 28, 2025
- Editors: F. Javier Alvarez-Leefmans, Eric Delpire, Edna Kaneshiro
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 8 1 1 1 1 4 - 7
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 1 1 1 1 5 - 4
Cell Physiology Source Book, Fifth Edition covers a broad range of topics in cell physiology. The book discusses research areas that have become active since the last edition (e.… Read more
Cell Physiology Source Book, Fifth Edition covers a broad range of topics in cell physiology. The book discusses research areas that have become active since the last edition (e.g., aquaporins, apicoplast and other organelles) and broadens its scope to include chronobiology, expansion of receptor/sensory physiology, endocrinology, and other topics such as quorum sensing and taxis. As methods or approaches on performing experiments appear to be very valuable parts of books to which readers tend to frequently refer, expansion of these types of chapters and/or appendices are included in this edition.
Applicable to scientists, researchers, postdocs and graduate students across physiological, biochemical, biological and biomedical backgrounds, cell physiology is important for understanding larger organisms and potential advances in biomedicine.
- Authored by leading experts in the field
- Illustrated with high-quality color images
- A valuable resource for researchers and students in biomedical sciences, covering fundamental and advanced cellular and molecular physiology and biophysics topics
- The book's content reflects current trends and developments in the field, making it an essential read for those seeking a deeper understanding of cell function
- Clear, concise, and comprehensive, the Cell Physiology Sourcebook is designed to complement medical physiology and biology textbooks. It provides a deeper dive into key concepts, making it an invaluable resource for students and researchers in biomedical sciences
Graduate students, postdocs, early and experienced researchers, and professionals studying cell physiology, cell biology, biophysics, developmental biology, biochemistry, stem cells, immunology, and medicine
SECTION I. Biophysical Chemistry, Cell Structure and Function
1. Water, Solutions, and Colligative Properties, Simple Diffusion, Permeability2. Lipids Artificial Membranes, Ionophores, Lipid Rafts, Liposomes3. Nucleic Acids Nucleus, Nucleolus, DNA, Chromosomes, Mitotic Spindle, Plasmids, Organellar DNA, Telomeres, DNA and RNA Editing, also see chapter 8)4. Proteins Cytoskeleton, Enzymes, Carriers, Protein Folding, Protein Crystallography5. Carbohydrates Glycocalyx, VSG, Mucins (e.g., T. cruzi), Receptors (Immune System, Lectins)6. Cell Membranes Bilayer Model, Lateral Mobility (Fluorescence Recovery After Photobleaching)7. Prokayotic Cells (eubacteria, archaea)8. Protists kDNA, Self-splicing, telomeres, apicoplasts, hydrogenosomes, mitosomes9. Organelles
SECTION II. Membrane Potentials, Transmembrane Translocation
10. Membrane Potentials and Membrane Transport Gibbs-Donnan Equilibrium, Ion Channels in Non-Excitable Membranes, (Mutations, Disease, Toxin- & Drug-Binding)11. Carrier-Mediated Transport/Facilitated Diffusion Overview Ions (Na+, K+, Cl-), Sugars, Amino Acids, Nutrients, Drugs12. Na+/K+ ATPases13. Ca2+ Transport and Signaling (Surface Membrane, Cytosolic, Nuclear)14. Proton-Motive Force Ion Gradients, Chemosmotic Energy Transduction, Bacterial Flagella, Mitochondrial/Chloroplast Membrane Potential and Energy Production
SECTION III. Membrane Excitability, Regulation of Ion Channels
15. Cable Properties, Length Constant, Electrogenesis of Membrane Excitability, Propagation of Action Potentials, Inhibition16. Voltage and Current Clamp (e.g., Oocytes, BLM), Patch-Clamp, Single Channel Techniques17. Voltage-Gated Channels18. Regulation of Mechanosensitive Channels, GTP, Cyclic nucleotides, G-proteins19. Nuclear Envelope
SECTION IV. Integration, Synaptic Transmission, Sensory Transduction
20. Ligand-Gated Ion Channels21. Synaptic Transmission, Gap and Tight Junctions22. Excitation-Secretion Coupling, Trophic Effects of Nerves on Muscles? (see Endocrine?)23. Photoreceptors: Vision, Eye spots, Ocelli, Compound Eyes, Color Vision24. Mechanoreceptors: Sound, Balance, Stretch Receptors Pacinian Corpuscle, Hearing25. Pressure Receptors: Posture, Acceleration, Fish Lateral Line26. Chemoreceptors: Smell, Taste27. Electroreceptors, Magnetoreceptors (Magnetosomes), Thermoreceptors28. Brain, Glia
SECTION V. Contractile Systems, Cell Locomotion, Muscle
28. Microtubule + Dynein + ATPase, Eukaryote Cilia39. Proton-Motive Force, Bacterial Flagella31. Spasmin/centrin + Ca2+, Rubber?32. Actin +Myosin + ATPase, Amoeboid Movements, Lymphocytes33. Skeletal Muscle, Excitation-Contraction Coupling, Sarcoplasmic Reticulum, Mechano-Chemistry, Insect click flight muscle?34. Smooth Muscle35. Cardiac Muscle
SECTION VI. Integration, Endocrine, Exocrine Systems, Chemical Messengers, Hormones
36. Pituitary/Hypothalamus37. Adrenals38. Reproductive Physiology39. Respiration, Gas Transport, Respiratory Pigments, Acid-Base Balance40. Osmoregulation, Volume Regulation, Aquaporin, Ice/Freezing, Counter- Current Systems, Counter-Current Multiplier (Active Transport) Systems
SECTION VII. SPECIALIZDED PROCESSES
41. Bioluminescence42. Plant Cell Physiology, Plastids, Photosynthesis, Stomata Opening43. Cell Migration, Quorum Sensing, Taxis, Kinesis, Tropism44. Biological Clocks/Chronobiology, Diapause, Hibernation, Brown Fat
SECTION VIII. Experimental Methods/Approaches
Cell Biology – Microscopy, GFP, Specific Stains, In Situ Hybridization, Light, Phase-Contrast; Fluorescence Microscopy, Fluorescence Resonance Energy Transfer (FRET); Multi-Photon, Electron Microscopy, Confocal, Imaging, FISH, Atomic Force, STORM/Single Molecule Imaging, Photoactivated Localization Microscopy (PALM); Total Internal Reflection Microscopy (TIRF), Fluorescence Recovery After PhotobleachingMolecular Biology –Nucleotide Sequencing, Whole Genome Analysis, Mutagenesis, Recombinant DNA, PCR, SDS-PAGE, Western Blots, Expression Systems, Antibody Preparation. Biochemistry - Enzyme Kinetics; Gas-Liquid Chromatography, Mass Spectrometary, Nuclear Magnetic Resonance Spectroscopy, Fourier Transforms, X-ray Scattering, Electron Spin Resonance SpectroscopyBiophysical – Optical Tweezers, Magnetic Resonance ImagingHigh Throughput – Bioinformatics, Genomics, Proteomics
- Edition: 5
- Published: November 28, 2025
- Language: English
FA
F. Javier Alvarez-Leefmans
Research Interests Research in his laboratory focuses on the molecular and cellular physiology of carrier protein molecules that actively transport chloride ions (Cl-) across the plasma membrane of neurons and epithelial cells. Specifically, they study some members of the cation-coupled-chloride contransporter gene/protein family SLC12A: the Na+, K+, 2 Cl- cotransporters (NKCC1 and NKCC2) and the K+-Cl- cotransporters (KCC1, 2, 3 and 4). These carrier proteins play key roles in: intracellular Cl- homeostasis in neurons, GABA- and glycine-mediated synaptic signaling, neuronal development, sensory transduction including nociception, transepithelial salt transport, cell water volume control, and extracellular K+ scavenging. Not surprisingly, altered function of these proteins underlies several pathologies and hence they have become significant targets for therapeutic interventions and translational research. To study the function of these proteins we use state-of-the-art live-cell imaging microscopy and fluorescent probes for measuring and manipulating intracellular ions and water in dissociated neurons and epithelial cells. Some of these optical methods have been developed in their lab, and are used in conjunction with molecular methods, knockout models, and several microanatomical techniques. Their current research involves two projects: Mechanisms regulating intracellular chloride in primary afferent neurons and their impact on GABA-mediated presynaptic inhibition and sensory transduction. This project aims at understanding the molecular mechanisms that determine intracellular Cl- concentration in primary afferent neurons, their regulation, and the role they play in presynaptic inhibition, acute somatic pain, neurogenic inflammation and proprioception. Roles of cation-coupled-chloride contransporters of choroid plexus epithelial cells in the regulation of cerebrospinal fluid ion composition. The choroid plexus epithelial cells (CPECs) form the blood-cerebrospinal fluid (CSF) barrier. CPECs secrete CSF and regulate its electrolyte composition. Regulation of CSF ion levels is fundamental for maintaining normal brain function. The overarching goal of this project is to understand how NKCC1, KCCs and aquaporins control the ion composition of the cerebrospinal fluid. Current emphasis is on the molecular and cellular mechanisms used by CPECs to regulate and maintain the CSF K+ concentration, a fundamental problem of broad physiological significance. CSF composition has a major impact on the fluid microenvironment of neurons and glial cells, and vice versa. Extracellular K+ homeostasis is critical for normal brain function; small changes in extracellular K+ profoundly affect neuronal excitability and osmotic water balance of glial cells and neurons.
Affiliations and expertise
Department of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University, USAED
Eric Delpire
Dr. Eric Delpire teaches at the Vanderbilt University, Nashville, USA
Affiliations and expertise
Vanderbilt University, Nashville, Tennessee, USAEK
Edna Kaneshiro
Edna Kaneshiro is a distinguished research professor in the department of biology at the University of Cincinnati where she has been for 43 years. Dr. Kaneshiro’s research is on the lipids of eukaryote protists, including free living, parasitic, and opportunistic pathogens. Although work on a number of different protozoa is being performed, Kaneshiro’s current focus is on the AIDS-associated opportunistic infection caused by Pneumocystis carinii. This organism causes a type of pneumonia that can lead to the death of immunocompromised individuals. Pneumocystis proliferates extracellularly in the lung alveolus where lipids constitute a major part of lung surfactant. Thus, lipids are thought to be important to the nutrition, physiology and metabolism of the organism. The biosynthesis of pathogen-specific lipids represents potential targets for drug development. She also has an interest in the cell biology and life history of this poorly understood opportunistic infectious agent. Dr. Kaneshiro is an elected fellow of the American Association for the Advancement of Science and the American Academy of Microbiology
Affiliations and expertise
Distinguished Research Professor, Department of Biology, University of Cincinnati, USA