Cell of Immunoglobulin Synthesis

List of Participants

Preface

Opening Address

B Lymphocyte Differentiation and the Tolerance Problem

Sequence of Acquisition of la, IgM, and IgD in Adult Murine Bone Marrow

Kinetics of Tolerogenesis among Immature B Cells of Various Categories

The Cambier Dilemma — Differences between T-Independent and T-Dependent Cells

Multivalency of Tolerogens and the Nature of Tolerance Phenomena in Adult Animals

Conclusions

References

Part I Immunoglobulin Genes, Messages, and Molecules

Somatic Recombination and Structure of an Immunoglobulin Gene

Introduction

Scheme for Isolation of Ig Genes from Cellular DNA

DNA Clones Isolated from Embryo and Myeloma Cells

Type of Gene Sequences Contained in the DNA Clones

Location of the λ Chain Gene Sequence in the DNA Clones

Sequence Homology between the Cloned DNA Fragments

Evidence for Somatic Rearrangement of Immunoglobulin Genes

References

Implications of the Assortment of Framework Segments for the Assembly of Immunoglobulin VL and VH Regions and the Generation of Diversity

Text

References

Immunoglobulin Genes and Nuclear RNA Precursors

Introduction

Results and Discussion

Closing Remarks

References

Studies on mRNA Sequence and Immunoglobulin Gene Organization Using Synthetic Oligonucleotides

Introduction

Studies with 32P-mRNA and cDNA Primed with Oligo(dT)

Studies with Specific Primers

The Uses of mRNA Nucleotide Sequence Data

Hybrid Myeloma Cells and the Specificity of V-C Integration

Summary

References

Comparative Aspects of in Vitro and Cellular Assembly of Immunoglobulins

Text

References

Human Immunoglobulin Mutants

Text

References

Part II Cellular Immunoglobulin Production

Variable and Constant Region Variants of Mouse Myeloma Cells

Introduction

Variants with Changes in Antigen Binding

Constant Region Mutants

Conclusions

References

Constant Region Mutants of Mouse Immunoglobulins

Introduction

Isolation and Characterization of 10-1, a Mutant Cell Line Synthesizing an Internally

Deleted Heavy Chain

Mutants Derived from the 10-1 Cell Line

Subclass Switch Mutants from G251 and 117

Mechanisms of Subclass Switch

References

Differentiation of Leukemic B Lymphocytes in Man

Introduction

In Vivo Differentiation

Induction of in Vitro Differentiation of Leukemic B Lymphocytes

Epstein-Barr Virus Transformation of Leukemic B Lymphocytes

Defective T Cell Helper Function in Patients

Discussion and Conclusion

References

Intracellular Events in the Differentiation of B Lymphocytes to Pentamer IgM Synthesis

Text

References

Part III Membrane Immunoglobulins

Receptor-Mediated Triggering and Tolerance in Murine B Cells

The Role of Surface Igs in B Cell Tolerance

The Role of Surface Igs in B Cell Triggering

References

Structure of Lymphocyte Membrane Immunoglobulin

Introduction

Methods

Results

Discussion

References

Role of Membrane Immunoglobulins in Lymphocyte Responses

Introduction

Effect of Anti-Immunoglobulin Antibodies on B Lymphocyte Maturation Induced by LPS

Effect of Antigen on B Lymphocyte Maturation Induced by LPS

Summary

References

Part IV Immunoglobulins as Regulatory Molecules

Function of Surface Immunoglobulin of B Lymphocytes

Capping

Endocytosis of Ig-Ligand Complexes

Receptor Reexpression

Modulation of Proliferation

References

The Role of Immunoglobulin in the Induction of B Lymphocytes

Introduction

Results

Discussion

References

Role of the Fc Portion of Antibody in Immune Regulation

Introduction

Activation of B Lymphocytes by the Fc Portion of Immunoglobulin

Summary

References

Factors Affecting the Triggering of the B-Cell Repertoire

Introduction

The Clonotype Repertoire

B-Cell Subpopulations

Antigen Form

Modes of Antigenic Stimulation

Suppression

Conclusion

References

Part V Lymphocyte Hybrids

Fusion of Immunoglobulin Secreting Cells

Introduction

Hybridomas

Cellular Restrictions on Hybridoma Fusion

Molecular Biology of Immunoglobulins

Basis of Differentiation

References

Analysis of the Diversity of Murine Antibodies to α(1→3) Dextran

Introduction

Extent of Diversity of Anti-α(1→3) Dextran Repertoire

Correlation between Spectrotype, Idiotype, and Isotype

Somatic Cell Hybrids

Summary

References

Monoclonal Antibodies to Alloantigens and to Immunoglobulin Allotypes

Introduction

Production of Hybrids

Antibodies to Alloantigens of the Major Histocompatibility Complex

Purification and Chain Composition of Hybrid Cell Antibodies

Antibodies to Immunoglobulin Allotypes

Conclusions

References

Part VI Immunoglobulin Idiotypes

Idiotype-Specific Suppression Mediated by B Cells

Introduction

Results and Discussion

References

Regulation of the Immune System by Idiotype-Anti-Idiotype Interaction in the Rabbit and the Mouse

Introduction

Idiotypic Manipulation of the Mouse Immune System

VH Allotypic Background and Network

Comparison between Idiotype of Ab1 and Idiotype of Ab3

Conclusion

References

Ontogeny of Clonal Dominance

Introduction

Loss of Clonal Dominance after Adoptive Transfer

Rescue of Clonal Dominance

Discussion

Summary

References

Anti-(T,G)-A--L Idiotypes: Initial Studies of Genetic Control and Cellular Expression

Introduction

Production and Specificity of Antisera Directed against Anti-(T,G)-A--L Idiotypes

Genetic Control of the Expression of BIO Anti-(T,G)-A--L Idiotype(s)

Cellular Expression of Anti-(T,G)-A--L Idiotype(s)

References

Part VII Ontogeny of Immunoglobulin-Synthesizing Cells

B Lymphocyte Development and Activation: Analysis with a Mutant Mouse Strain

Introduction

Conclusion

References

Influence of Thymus Cells on the Ontogeny of B Lymphocyte Function

Introduction

Methods and Rationale

Results and Discussion

Conclusions

References

Pre-B Cells: Normal Morphologic and Biologic Characteristics and Abnormal Development in Certain Immunodeficiencies and Malignancies

Introduction

Historical Perspective of the Identification of Pre-B Cells

Ontogeny, Morphology, and Organ Distribution of Pre-B Cells

Growth Characteristics of Pre-B Cells

Evidence for Precursor-Progeny Relationship of Pre-B Cells and B Lymphocytes

Surface and Cytoplasmic Constituents of Pre-B Cells

Absence of Functional Responses of Pre-B Cells

Evidence for Clonal Diversity of Pre-B Cells

Pre-B Cells in Immunodeficiencies

Malignancies Involving Pre-B Cells

Conclusion

References

Index