Cell Biological Applications of Confocal Microscopy
- 1st Edition, Volume 38 - October 19, 1993
- Latest edition
- Editors: Brian Matsumoto, Leslie Wilson, Paul T. Matsudaira
- Language: English
Cell Biological Applications of Confocal Microscopy instructs researchers on methods of preparing biological material for confocal microscopy. Individual chapters describe the te… Read more
Description
Description
Cell Biological Applications of Confocal Microscopy instructs researchers on methods of preparing biological material for confocal microscopy. Individual chapters describe the technical problems of imaging structures in thick specimens and useful techniques such as multi-color dye visualization. The book provides specific examples of applications of confocal microscopy to cell biological problems. This volume is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities.
Key features
Key features
- Describes proper specimen preparation for obtaining high-quality data and images
- Covers pitfalls in imaging different specimens and in employing different dye
- Contains detailed protocols accessible to new users
- Chapters are self-contained units, but describe strategies that can be combined to develop new protocols
- Techniques are illustrated with full-color plates
Readership
Readership
New and experienced researchers, graduate students, and technicians in cell and developmental biology, anatomy, pathology, neuroscience, biophysics, optical science. Also large audience in other biological areas for which microscopy is an important research tool, such as directors and technical staff of microscopy and imaging facilities.
Table of contents
Table of contents
S.J. Wright, V.E. Centonze, S.A. Stricker, P. Devries, S.W. Paddock, and G. Schatten, An Introduction to Confocal Microscopy and Three-Dimensional Reconstruction.J.J. Art and M.B. Goodman, Rapid Scanning Confocal Microscopy.L. Majlof and P.-O. Forsgren, Confocal Microscopy: Important Considerations for Accurate Imaging.T.C. Brelje, M.W. Weesendorf, and R.L. Sorenson, Multi-Color Laser Scanning Confocal Immunofluorescence Microscopy: Practical Application and Limitations.I. Kurtz and C. Emmons, Measurement of Intracellular pH with a Laser Scanning Confocal Microscope.L.M. Loew, Confocal Microscopy of Potentiometric Fluorescent Dyes.M. Terasaki and L. Jaffe, Imaging the EndoplasmicReticulum in Living Sea Urchin Eggs.A.H. Cornell-Bell, I.R. Otake, K. Sadler, P.G. Thomas, S. Lawrence, K. Olsen, F.R. Gumkowski, J.R. Peterson, and J.D. Jamieson, Membrane Glycolipid Trafficking in Living, Polarized Pancreatic Acinar Cells: Assessment by Confocal Microscopy.D.L. Gard, Confocal Immunofluorescence Microscopy of Microtubules in Amphibian Oocytes and Eggs.R.G. Summers, S.A. Stricker, and R.A. Cameron, Applications of Confocal Microscopy to Studies of Sea Urchin Embryogenesis.J.I. Hale and B. Matsumoto, Resolution of Subcellular Detail in Thick Tissue Sections: Immunohistochemical Preparation and Fluorescence Confocal Microscopy.G.E. Baker and B.E. Reese, Using Confocal Laser Scanning Microscopy to Investigate the Organization and Development of Neuronal Projections Labeled with DiI.J.N. Turner, J.W. Swann, D.H. Szaroski, K.L. Smith, D.O. Carpenter, and M. Fejtl, Three-Dimensional Confocal Light Microscopy of Neurons: Fluorescent and Reflection Stains.Chapter References.Subject Index.
Review quotes
Review quotes
"The diverse applications presented in this book reflect the creativity and technical skills of the many contributors while emphasizing the power and promise of CM."—TRENDS IN CELL BIOLOGY
Product details
Product details
- Edition: 1
- Latest edition
- Volume: 38
- Published: December 7, 1993
- Language: English
About the editors
About the editors
BM
Brian Matsumoto
Affiliations and expertise
Neuroscience Research Institute, University of California, Santa Barbara, U.S.A.LW
Leslie Wilson
University of California, Santa Barbara, USA
Affiliations and expertise
University of California, Santa Barbara, USAPM
Paul T. Matsudaira
Affiliations and expertise
Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, U.S.A.