Celiac Disease
- 1st Edition, Volume 179 - August 21, 2023
- Editors: Ainara Castellanos-Rubio, Lorenzo Galluzzi
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 3 - 1 9 2 0 2 - 9
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 1 9 2 0 3 - 6
Celiac Disease, Volume 179 in the Methods in Cell Biology series is composed of extensive protocols about novel and widely used techniques for celiac disease research. Topics… Read more
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Request a sales quoteCeliac Disease, Volume 179 in the Methods in Cell Biology series is composed of extensive protocols about novel and widely used techniques for celiac disease research. Topics covered in this volume include Pepsin trypsin digested gliadin treatment of intestinal cells in vitro, Ex vivo Gliadin Stimulation of Intestinal Cells, Measuring intestinal permeability in celiac disease ex vivo using Ussing chambers, Intestinal permeability assessment using lactulose and mannitol, In vivo sensitization to gliadin by oral administration, Separation of epithelial and immune cells from intestinal biopsy samples, Isolation and study of dendritic cells and macrophages from human intestinal samples, Gnobiotic mouse models to study gluten sensitivity, and more.
Other sections in this new release include In vitro differentiation of macrophages from peripheral blood cells of celiac patients, Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a three-day gluten challenge, Isolation, cryopreservation and thawing of peripheral blood mononuclear cells for downstream applications, and more.
- Provides up-to-date and extensive protocols for the research on different aspects of celiac disease pathogenesis
- Includes original figures which help users understand and follow the described protocols
- Covers research on different aspects of disease, covering a wide range of technologies
Academic, researchers and clinical gastroenterologists interested on celiac disease
- Cover image
- Title page
- Table of Contents
- Series Page
- Copyright
- Contributors
- Chapter 1: Pepsin-trypsin digested gliadin treatment in intestinal cells
- Abstract
- 1: Introduction
- 2: Cell maintenance
- 3: PTG treatment
- 4: RNA and protein extraction and analysis
- 5: Concluding remarks
- 6: Notes
- Acknowledgments
- References
- Chapter 2: Ex vivo gliadin stimulation of intestinal cells
- Abstract
- 1: Introduction
- 2: Reagents
- 3: Mouse intestinal epithelial cell extraction
- 4: Gliadin stimulation
- 5: Concluding remarks
- References
- Chapter 3: Measuring intestinal permeability in celiac disease ex vivo, using Ussing chambers
- Abstract
- 1: Introduction
- 2: Protocol
- 3: Additional notes
- Acknowledgments
- References
- Chapter 4: Intestinal permeability assessment using lactulose and mannitol in celiac disease
- Abstract
- 1: Introduction
- 2: The lactulose and mannitol (LM) test
- 3: Sugar analysis by ion chromatography using the technique High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD)
- 4: Interpretation of results
- 5: Concluding remarks
- 6: Notes
- Acknowledgments
- References
- Chapter 5: In vivo sensitization to gliadin by oral administration
- Abstract
- 1: Introduction
- 2: Animals
- 3: Reagents
- 4: Gliadin sensitization process
- 5: Validation of the gliadin sensitization process
- 6: Notes
- Acknowledgments
- References
- Chapter 6: Separation of epithelial and immune cells from biopsy samples
- Abstract
- 1: Introduction
- 2: Biopsy sample collection and processing
- 3: Removal of dead cells
- 4: Leucocyte labeling
- 5: Magnetic separation
- 6: Sample storage
- 7: Labeling for flow cytometry
- 8: Concluding remarks
- 9: Notes
- Acknowledgments
- References
- Chapter 7: Study and isolation of human intestinal dendritic cell and macrophage subsets
- Abstract
- 1: Introduction
- 2: Study of DC and MΦ from intestinal biopsies
- 3: DC and MΦ isolation from intestinal resections
- 4: Notes
- Acknowledgments
- References
- Chapter 8: Elucidating the role of microbes in celiac disease through gnotobiotic modeling
- Abstract
- 1: Introduction
- 2: Animal models of celiac disease
- 3: Axenic and gnotobiotic models to study celiac disease
- 4: Generation, maintenance and monitoring of germ-free mice
- 5: Colonization of germ-free mice
- 6: Conclusions
- References
- Chapter 9: In vitro differentiation of macrophages from peripheral blood cells of celiac patients
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Concluding remarks
- References
- Chapter 10: Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a 3-day gluten challenge
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Concluding remarks
- 5: Notes
- Acknowledgments
- Competing interests
- References
- Chapter 11: Isolation and cryopreservation of peripheral blood mononuclear cells
- Abstract
- 1: Introduction
- 2: Materials (see Note 1)
- 3: Methods
- 4: Concluding remarks
- Competing interests
- References
- Chapter 12: Flow cytometric analysis of duodenal intraepithelial lymphocytes (celiac lymphogram): A diagnostic test for celiac disease
- Abstract
- 1: Introduction
- 2: Materials (see Note 1)
- 3: Methods
- 4: Interpretation
- 5: Notes
- 6: Concluding Remarks
- Acknowledgments
- References
- Chapter 13: The HLA-DQ8 transgenic mouse: A model to study the immune and cytotoxic responses to wheat gliadin
- Abstract
- 1: Introduction
- 2: The HLA-DQ8 transgenic mouse model
- 3: Induction of the enteropathy
- 4: Induction of the intestinal immune response
- 5: Cell culture
- 6: Analyses of selected cytokines (IFN-γ, IL-10, IL-17)
- 7: Morphology assessment
- 8: Immunostaining of cryostat sections
- 9: Concluding remarks
- 10: Notes
- Acknowledgments
- References
- Chapter 14: Human intestinal organoid models for celiac disease research
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Notes
- 5: Troubleshooting
- 6: Concluding remarks
- Acknowledgments
- Competing interests
- References
- Chapter 15: Preparation of pepsin trypsin digested gliadin for stimulation experiments
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Notes
- References
- Chapter 16: A fast, cheap, and easy protocol for celiac disease HLA haplotype typing using buccal swabs
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: HLA haplotype determination
- Notes
- 5: Concluding remarks
- References
- No. of pages: 318
- Language: English
- Edition: 1
- Volume: 179
- Published: August 21, 2023
- Imprint: Academic Press
- Hardback ISBN: 9780443192029
- eBook ISBN: 9780443192036
AC
Ainara Castellanos-Rubio
Ainara Castellanos-Rubio obtained her PhD on Genetics from the University of the Basque Country (Spain) in 2010. During her PhD, she studied gene expression alterations and genetic polymorphisms associated to Celiac Disease. She did a short term research stay in the University of Tampere (Finland) under the supervision of Dr. Marku Makki and Dr. Katri Lindfors where she used three dimensional cell cultures to describe different pathways involved in Celiac Disease development. On 2011 she joined the Laboratory of Dr. Sankar Ghosh in Columbia University (NY, USA) where she carried out her postdoctoral studies. During her postdoctoral training, she studied the implication of long noncoding RNAs (lncRNAs) in the immune and inflammatory response and she discovered and functionally characterized a novel lncRNA involved in the susceptibility to Celiac Disease. Dr. Castellanos-Rubio is an Ikerbasque Associate in the University of the Basque Country, where she leads her own group. She is interested in the involvement of noncoding RNAs in the pathogenesis of autoimmune and inflammatory diseases and studies the influence of disease associated SNPs in the functional disturbance of these RNAs. More recently, she has become interested on the epitranscriptomic alterations involved in different aspects of RNA regulation and her group studies how SNPs and environmental factors can alter these epitranscriptomic signals influencing the inflammatory response that finally evolves in disease development.
Affiliations and expertise
University of the Basque Country and Ikerbasque, Basque Foundation for Science, Leioa, SpainLG
Lorenzo Galluzzi
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.
Affiliations and expertise
Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USARead Celiac Disease on ScienceDirect