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Catalytic RNA
- 1st Edition, Volume 120 - October 21, 2013
- Editor: Garrett A. Soukup
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 3 8 1 2 8 6 - 5
- eBook ISBN:9 7 8 - 0 - 1 2 - 3 8 1 2 8 7 - 2
This special volume of Progress in Molecular Biology and Translational Science focuses on catalytic RNA. Written by experts in the field, the reviews cover a range of topics… Read more
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Researchers, professors and graduate students in biochemistry, chemistry, molecular biology, biotechnology, and medicine.
Contributors
Preface
Chapter One. The Hammerhead Ribozyme: Structure, Catalysis, and Gene Regulation
Abstract
1 Discovery and Context
2 The Minimal Sequence
3 The Full-Length Sequence
4 Hammerhead Ribozymes in Mammalian Gene Regulation
References
Chapter Two. Bridging the Gap Between Theory and Experiment to Derive a Detailed Understanding of Hammerhead Ribozyme Catalysis
Abstract
1 Introduction
2 A Metal Ion Story with a Happy Ending
3 Finding the Catalytic Reaction Path Through Multidimensional Free-Energy Surfaces
4 HHR Uses Specific Cation-Binding Modes to Reach Its Catalytically Active Conformation
5 Computational Mutagenesis of Key Residues of HHR
6 Conclusion
Acknowledgments
References
Chapter Three. A Mechanistic Comparison of the Varkud Satellite and Hairpin Ribozymes
Abstract
1 Introduction
2 The Hairpin and VS Ribozymes
3 General Themes
Acknowledgments
References
Chapter Four. HDV Family of Self-Cleaving Ribozymes
Abstract
1 Introduction
2 Hepatitis Delta Virus
3 HDV Ribozymes
4 HDV Ribozymes’ Reaction Mechanism
5 Ribozymes Outside of the Hepatitis Delta Virus
6 Evolutionary Relationship Among HDV-Like Ribozymes
References
Chapter Five. The Structural and Functional Uniqueness of the glmS Ribozyme
Abstract
1 Introduction
2 The glmS Riboswitch/Ribozyme
3 Biological Function of the glmS Ribozyme
4 glmS Ribozyme Uniqueness
5 glmS Ribozyme Structure and Function—Biochemical Analyses
6 glmS Ribozyme Structure and Function—Crystallographic Analysis
7 Metal Ion Usage by the glmS Ribozyme
8 Essential Coenzyme GlcN6P Functional Groups
9 Mechanism of glmS Ribozyme Self-Cleavage Using Coenzyme GlcN6P
10 pH-Reactivity Profiles Provide Mechanistic Insight
11 Potential for Antibiotic Development Affecting glmS Ribozyme/Riboswitch Function
Acknowledgments
References
Chapter Six. The Role of snRNAs in Spliceosomal Catalysis
Abstract
1 Evolution of Eukaryotic Splicing Machines
2 Structural and Functional Similarities Between snRNAs and the Group II Self-Splicing Introns
3 Role of snRNAs in the Spliceosomal Active Site
4 The Three-Dimensional Positioning of snRNAs in the Catalytic Core
5 An RNA-Centric View of the Active Site
6 Functionally Critical snRNA–Metal Ion Interactions
7 Catalytic Activity of the snRNAs in Isolation
8 The snRNAs in the Context of the Spliceosomal Active Site
9 The Role of Proteins in the Spliceosomal Catalytic Core
10 The Role of Spliceosomal Proteins in Remodeling the Catalytic Core
11 Concluding Remarks
References
Index
- No. of pages: 248
- Language: English
- Edition: 1
- Volume: 120
- Published: October 21, 2013
- Imprint: Academic Press
- Hardback ISBN: 9780123812865
- eBook ISBN: 9780123812872
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