Carboxylases
- 1st Edition, Volume 708 - November 21, 2024
- Imprint: Academic Press
- Editor: Martin ST. Maurice
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 3 - 3 1 3 1 0 - 3
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 3 1 3 1 1 - 0
carboxylases series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of… Read more
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Request a sales quotecarboxylases series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
- Provides the latest information on carbon enzymes research
- Offers outstanding and original reviews on a range of carbon enzymes research topics
- Serves as an indispensable reference for researchers and students alike
Provides invaluable information on the fast-moving field of carbon enzymes research that includes outstanding original reviews on a variety of topics
- Title of Book
- Cover image
- Title page
- Table of Contents
- Series Page
- Copyright
- Contributors
- Preface
- Chapter One: Purification and characterization of mitochondrial biotin-dependent carboxylases from native tissues
- Abstract
- 1 Introduction
- 2 Before you begin
- 3 Key resources table
- 4 Materials and equipment
- 5 Step-by-step method details
- 6 Expected outcomes
- 7 Advantages
- 8 Limitations
- 9 Optimization and troubleshooting
- 10 Safety considerations and standards
- 11 Alternative methods/procedures
- 12 Summary and conclusions
- References
- Chapter Two: Sample optimizations to enable the structure determination of biotin-dependent carboxylases
- Abstract
- 1 Introduction
- 2 Sample optimizations for structural studies of yeast ACC
- 3 Sample optimization for crystal structure determination of PCC
- 4 Homologous domains are assembled to give highly distinct overall architectures of the biotin-dependent carboxylases
- 5 Summary
- Acknowledgments
- References
- Chapter Three: Purification of heteromeric acetyl-CoA carboxylases from Escherichia coli for structure solution
- Abstract
- 1 Introduction
- 2 Expression construct design
- 3 ACC complex overproduction in E. coli
- 4 Purification of the ACC complex
- 5 Activity assay by HPLC
- 6 Summary and conclusions
- Acknowledgments
- References
- Chapter Four: Insights into the methodology of acetyl-CoA carboxylase inhibition
- Abstract
- 1 Background
- 2 Inhibitors for analysis of ACC structure and function
- 3 Inhibitors of ACC with antibacterial properties
- 4 Biotin carboxylase
- 5 Biotin binding site
- 6 Unknown binding site
- 7 Carboxyltransferase
- 8 Concluding remarks
- Author note
- References
- Chapter Five: Characterization of guanidine carboxylases
- Abstract
- 1 Introduction
- 2 Before you begin
- 3 Key resources table
- 4 Materials and equipment
- 5 Step-by-step method details
- 6 Limitations
- 7 Optimization and troubleshooting
- 8 Safety considerations and standards
- 9 Conclusion
- References
- Chapter Six: Methods to study prFMN-UbiD mediated (de)carboxylation
- Abstract
- 1 Introduction
- 2 In vivo production of holo-UbiD/apo-UbiD
- 3 In vitro reconstitution of apo-UbiD using prFMNreduced
- 4 Synthesis of DMAP
- 5 In vitro oxidative maturation of prFMN containing UbiD
- 6 Characterisation of prFMN species via ESI-MS
- 7 1H NMR monitored enzyme catalysed deuterium exchange
- 8 (De)carboxylation assays monitored by HPLC/LC-MS
- 9 Preparation of whole-cell UbiD-biocatalysts
- 10 UbiD-substrate screening through monitoring flavin spectral shift
- 11 Experimental strategies to drive carboxylation
- 12 Conclusion
- References
- Chapter Seven: Enzyme cascades for in vitro and in vivo FMN prenylation and UbiD (de)carboxylase activation under aerobic conditions
- Abstract
- Abbreviations
- 1 Introduction
- 2 In vitro biosynthesis of prFMN and UbiD activation
- 3 Procedure: in vivo FMN prenylation and UbiD activation using recombinant E. coli cells
- 4 Conclusions
- Acknowledgments
- References
- Chapter Eight: Cellular and biochemical approaches to define GGCX carboxylation of vitamin K-dependent proteins
- Abstract
- 1 Introduction
- 2 Cellular analysis of VKD protein carboxylation
- 3 Biochemical analysis of VKD protein carboxylation
- 4 Considerations
- References
- Chapter Nine: Assessment of gamma-glutamyl carboxylase activity in its native milieu
- Abstract
- 1 Introduction
- 2 Cell-based assessment of VKD carboxylation activity using ELISA
- 3 Functional study of naturally occurring GGCX mutations in the cellular milieu
- 4 Assessment of vitamin K epoxidase activity in the cellular milieu
- 5 Summary and conclusions
- Acknowledgments
- References
- Chapter Ten: Expression, purification, and activation of one key enzyme in anaerobic CO2 fixation: Carbon monoxide dehydrogenase II from Carboxydothermus hydrogenoformans
- Abstract
- 1 Introduction
- 2 Recombinant expression of CODH-II from C. hydrogenoformans
- 3 Assay for CODH-catalyzed CO oxidation
- 4 Optimization of growth conditions and lysis protocol
- 5 Summary
- Acknowledgments
- References
- Chapter Eleven: Molybdenum-containing CO dehydrogenase and formate dehydrogenases
- Abstract
- 1 Overview
- 2 CO Dehydrogenase
- 3 Formate dehydrogenases
- 4 Summary
- Acknowledgment
- References
- Chapter Twelve: Assessing the role of redox carriers in the reduction of CO2 by the oxo-acid: ferredoxin oxidoreductase superfamily
- Abstract
- 1 Introduction
- 2 General considerations
- 3 Materials and reagents
- 4 Assay design and implementation
- 5 Verification of redox potentials of Fd proteins
- 6 Comparison of assay results
- 7 Conclusions and future directions
- Acknowlegments
- References
- Chapter Thirteen: Measuring carbonic anhydrase activity in alpha-carboxysomes using stopped-flow
- Abstract
- 1 Introduction
- 2 Cultivation of H. neapolitanus
- 3 Purification of carboxysomes
- 4 Measuring carbonic anhydrase activity using stopped-flow
- 5 Data analysis
- 6 Summary
- Acknowledgment
- References
- Chapter Fourteen: Radiometric determination of rubisco activation state and quantity in leaves
- Abstract
- 1 Introduction
- 2 Methods
- 3 Summary and conclusion
- Acknowledgments
- References
- Further reading
- Chapter Fifteen: Computational methods for the study of carboxylases: The case of crotonyl-CoA carboxylase/reductase
- Abstract
- 1 Introduction
- 2 Molecular dynamics simulations identify conformational changes in crotonyl-CoA carboxylase/reductase increasing the local CO2 concentration in the active site
- 3 QM/MM molecular dynamics simulations reveal the reaction mechanism of the chemical CO2 fixation step
- 4 Summary
- References
- Chapter Sixteen: Carboxylation in de novo purine biosynthesis
- Abstract
- 1 Introduction
- 2 Carboxylation in de novo purine biosynthesis
- 3 CAIR synthesis as a drug target
- 4 Expression and purification of E. coli N5-CAIR mutase
- 5 Expression and purification of A. clavatus N5-CAIR synthetase
- 6 Synthesis of CAIR and AIR
- 7 Activity assays
- 8 Summary and conclusions
- References
- Edition: 1
- Volume: 708
- Published: November 21, 2024
- No. of pages (Hardback): 452
- No. of pages (eBook): 412
- Imprint: Academic Press
- Language: English
- Hardback ISBN: 9780443313103
- eBook ISBN: 9780443313110
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