SUSTAINABLE DEVELOPMENT
Innovate. Sustain. Transform.
Save up to 30% on top Physical Sciences & Engineering titles!
Carboxylases
- 1st Edition, Volume 708 - November 21, 2024
- Editor: Martin ST. Maurice
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 3 - 3 1 3 1 0 - 3
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 3 1 3 1 1 - 0
carboxylases series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of… Read more
Purchase options
Institutional subscription on ScienceDirect
Request a sales quotecarboxylases series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
- Provides the latest information on carbon enzymes research
- Offers outstanding and original reviews on a range of carbon enzymes research topics
- Serves as an indispensable reference for researchers and students alike
Provides invaluable information on the fast-moving field of carbon enzymes research that includes outstanding original reviews on a variety of topics
- Title of Book
- Cover image
- Title page
- Table of Contents
- Series Page
- Copyright
- Contributors
- Preface
- Chapter One: Purification and characterization of mitochondrial biotin-dependent carboxylases from native tissues
- Abstract
- 1 Introduction
- 2 Before you begin
- 3 Key resources table
- 4 Materials and equipment
- 5 Step-by-step method details
- 6 Expected outcomes
- 7 Advantages
- 8 Limitations
- 9 Optimization and troubleshooting
- 10 Safety considerations and standards
- 11 Alternative methods/procedures
- 12 Summary and conclusions
- References
- Chapter Two: Sample optimizations to enable the structure determination of biotin-dependent carboxylases
- Abstract
- 1 Introduction
- 2 Sample optimizations for structural studies of yeast ACC
- 3 Sample optimization for crystal structure determination of PCC
- 4 Homologous domains are assembled to give highly distinct overall architectures of the biotin-dependent carboxylases
- 5 Summary
- Acknowledgments
- References
- Chapter Three: Purification of heteromeric acetyl-CoA carboxylases from Escherichia coli for structure solution
- Abstract
- 1 Introduction
- 2 Expression construct design
- 3 ACC complex overproduction in E. coli
- 4 Purification of the ACC complex
- 5 Activity assay by HPLC
- 6 Summary and conclusions
- Acknowledgments
- References
- Chapter Four: Insights into the methodology of acetyl-CoA carboxylase inhibition
- Abstract
- 1 Background
- 2 Inhibitors for analysis of ACC structure and function
- 3 Inhibitors of ACC with antibacterial properties
- 4 Biotin carboxylase
- 5 Biotin binding site
- 6 Unknown binding site
- 7 Carboxyltransferase
- 8 Concluding remarks
- Author note
- References
- Chapter Five: Characterization of guanidine carboxylases
- Abstract
- 1 Introduction
- 2 Before you begin
- 3 Key resources table
- 4 Materials and equipment
- 5 Step-by-step method details
- 6 Limitations
- 7 Optimization and troubleshooting
- 8 Safety considerations and standards
- 9 Conclusion
- References
- Chapter Six: Methods to study prFMN-UbiD mediated (de)carboxylation
- Abstract
- 1 Introduction
- 2 In vivo production of holo-UbiD/apo-UbiD
- 3 In vitro reconstitution of apo-UbiD using prFMNreduced
- 4 Synthesis of DMAP
- 5 In vitro oxidative maturation of prFMN containing UbiD
- 6 Characterisation of prFMN species via ESI-MS
- 7 1H NMR monitored enzyme catalysed deuterium exchange
- 8 (De)carboxylation assays monitored by HPLC/LC-MS
- 9 Preparation of whole-cell UbiD-biocatalysts
- 10 UbiD-substrate screening through monitoring flavin spectral shift
- 11 Experimental strategies to drive carboxylation
- 12 Conclusion
- References
- Chapter Seven: Enzyme cascades for in vitro and in vivo FMN prenylation and UbiD (de)carboxylase activation under aerobic conditions
- Abstract
- Abbreviations
- 1 Introduction
- 2 In vitro biosynthesis of prFMN and UbiD activation
- 3 Procedure: in vivo FMN prenylation and UbiD activation using recombinant E. coli cells
- 4 Conclusions
- Acknowledgments
- References
- Chapter Eight: Cellular and biochemical approaches to define GGCX carboxylation of vitamin K-dependent proteins
- Abstract
- 1 Introduction
- 2 Cellular analysis of VKD protein carboxylation
- 3 Biochemical analysis of VKD protein carboxylation
- 4 Considerations
- References
- Chapter Nine: Assessment of gamma-glutamyl carboxylase activity in its native milieu
- Abstract
- 1 Introduction
- 2 Cell-based assessment of VKD carboxylation activity using ELISA
- 3 Functional study of naturally occurring GGCX mutations in the cellular milieu
- 4 Assessment of vitamin K epoxidase activity in the cellular milieu
- 5 Summary and conclusions
- Acknowledgments
- References
- Chapter Ten: Expression, purification, and activation of one key enzyme in anaerobic CO2 fixation: Carbon monoxide dehydrogenase II from Carboxydothermus hydrogenoformans
- Abstract
- 1 Introduction
- 2 Recombinant expression of CODH-II from C. hydrogenoformans
- 3 Assay for CODH-catalyzed CO oxidation
- 4 Optimization of growth conditions and lysis protocol
- 5 Summary
- Acknowledgments
- References
- Chapter Eleven: Molybdenum-containing CO dehydrogenase and formate dehydrogenases
- Abstract
- 1 Overview
- 2 CO Dehydrogenase
- 3 Formate dehydrogenases
- 4 Summary
- Acknowledgment
- References
- Chapter Twelve: Assessing the role of redox carriers in the reduction of CO2 by the oxo-acid: ferredoxin oxidoreductase superfamily
- Abstract
- 1 Introduction
- 2 General considerations
- 3 Materials and reagents
- 4 Assay design and implementation
- 5 Verification of redox potentials of Fd proteins
- 6 Comparison of assay results
- 7 Conclusions and future directions
- Acknowlegments
- References
- Chapter Thirteen: Measuring carbonic anhydrase activity in alpha-carboxysomes using stopped-flow
- Abstract
- 1 Introduction
- 2 Cultivation of H. neapolitanus
- 3 Purification of carboxysomes
- 4 Measuring carbonic anhydrase activity using stopped-flow
- 5 Data analysis
- 6 Summary
- Acknowledgment
- References
- Chapter Fourteen: Radiometric determination of rubisco activation state and quantity in leaves
- Abstract
- 1 Introduction
- 2 Methods
- 3 Summary and conclusion
- Acknowledgments
- References
- Further reading
- Chapter Fifteen: Computational methods for the study of carboxylases: The case of crotonyl-CoA carboxylase/reductase
- Abstract
- 1 Introduction
- 2 Molecular dynamics simulations identify conformational changes in crotonyl-CoA carboxylase/reductase increasing the local CO2 concentration in the active site
- 3 QM/MM molecular dynamics simulations reveal the reaction mechanism of the chemical CO2 fixation step
- 4 Summary
- References
- Chapter Sixteen: Carboxylation in de novo purine biosynthesis
- Abstract
- 1 Introduction
- 2 Carboxylation in de novo purine biosynthesis
- 3 CAIR synthesis as a drug target
- 4 Expression and purification of E. coli N5-CAIR mutase
- 5 Expression and purification of A. clavatus N5-CAIR synthetase
- 6 Synthesis of CAIR and AIR
- 7 Activity assays
- 8 Summary and conclusions
- References
- No. of pages: 452
- Language: English
- Edition: 1
- Volume: 708
- Published: November 21, 2024
- Imprint: Academic Press
- Hardback ISBN: 9780443313103
- eBook ISBN: 9780443313110
Read Carboxylases on ScienceDirect