Biotechnology Engineering
A Practical Approach on Bioprocess Development from Lab to Industrial Scale
- 1st Edition - August 26, 2025
- Latest edition
- Editors: Vinayaka B. Shet, Sandesh Kanthakere, Nabisab Mujawar Mubarak, Rama Rao Karri, Mohammad Hadi Dehghani
- Language: English
Biotechnology Engineering: A Practical Approach on Bioprocess Development from Lab to Industrial Scale offers a comprehensive guide on biotechnology engineering, focusing on transf… Read more
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Description
Description
The book is a vital resource for academics, scientists, engineers, and professionals in bioprocess development. It includes detailed discussions, calculations, and case studies, modern bioreactors, and software tools used in the industry. Serving as a desktop reference, it effectively combines theory with real-world applications to support the development of bioprocesses on all scales.
Key features
Key features
- Helps readers understand the practical approach involved in bioprocess developments
- Includes calculations and case studies that are essential for independently scaling up bioprocesses, from Erlenmeyer flask studies to bioreactors
- Provides insights into auditing requirements for the bioprocess industry
Readership
Readership
Table of contents
Table of contents
1: Culturing of microbial, animal and plant cells in lab scale
1.1 Introduction to Cell Culturing Techniques
1.2 Microbial Cell Culture: Techniques and Methods
1.3 Animal Cell Culture: Techniques and Methods
1.4 Plant Cell Culture: Techniques and Methods
1.5 Growth Kinetics and Cell Counting: Calculations for Assessment and Analysis
1.6 Conclusions and References
2: Microbial growth and cell kinetics
2.1 Introduction
2.2 General mass balance, elemental balance, and heat balance calculations with respect to fermenter/bioreactor
2.3 Microbial growth and production (linear growth equation, yield values), Enzymatic conversion
2.4 Kinetic Calculations: Growth, Substrate Utilization, and Product Formation
2.5 Application of Kinetic Models in Bioprocess Development
2.6 Case Studies and Examples of Material Balance and Kinetic Calculations
2.7 Conclusions and References
3: Design, optimization, and Inoculum development for culturing the cells
3.1 Basics of Media Design for Cell Culturing
3.2 Optimization Techniques for Media Formulation
3.3 Inoculum Development Strategies
3.4 Calculations and Considerations for Inoculum Scaling
3.5 Case Studies and Examples of Media Design and Inoculum Development
3.6 Conclusions and References
4: Lab scale studies on Submerged Liquid Fermentation (SLF) and Solid State Fermentation (SSF)
4.1 Introduction to Submerged Liquid Fermentation (SLF)
4.2 Lab Scale Studies and Experimental Setup for SLF
4.3 Key Parameters and Calculations in SLF
4.4 Introduction to Solid State Fermentation (SSF)
4.5 Lab Scale Studies and Experimental Setup for SSF
4.6 Comparative Analysis of SLF and SSF: Advantages and Limitations
4.7 Conclusions and References
PART B: Bioreactor handling and operation
5: Fundamental of Bioreactors and accessories
5.1 Introduction to Bioreactors: Types and Classification
5.2 Components and Functioning of Bioreactors
5.3 Measurement and Control Instruments in Bioreactors
5.4 Sensors and Probes for Monitoring Bioreactor Parameters
5.5 Auxiliary Equipment and Accessories in Bioreactor Systems
5.6 Conclusions and References
6: Design Aspects and Aseptic Maintenance of Bioreactors
6.1 Introduction
6.2 Bioreactor Design Considerations: Vessel Geometry and Configuration
6.3 Aseptic Design Principles for Bioreactors
6.4 Sterility Assurance and Maintenance in Bioreactor Systems
6.5 Cleaning and Sterilization of Bioreactor Components
6.6 Validation and Documentation of Aseptic Conditions in Bioreactors
6.7 Conclusions and References
7: Cleaning Techniques for Bioreactors and Regulatory Standards
7.1 Introduction
7.2 Importance of Cleaning in Bioreactor Operations
7.3 Cleaning Agents and Disinfectants for Bioreactors
7.4 Cleaning Validation Protocols and Procedures
7.5 Regulatory Standards for Bioreactor Cleaning
7.6 Case Studies and Best Practices for Effective Bioreactor Cleaning
7.7 Conclusions and References
8: Bioreactor sterilization techniques and equipment
8.1 Introduction
8.2 Principles and Importance of Bioreactor Sterilization
8.3 Sterilization Methods for Bioreactors: Heat, Chemical, and Radiation
8.4 Equipment and Tools for Bioreactor Sterilization
8.5 Sterility Testing and Monitoring in Bioreactors
8.6 Case Studies and Troubleshooting in Bioreactor Sterilization
8.7 Conclusions and References
9: Standard Operating Procedures and Auditing in Bioprocess Industries
9.1 Introduction
9.2 Importance of Standard Operating Procedures (SOPs)
9.3 Development and Implementation of SOPs in Bioprocess Industries
9.4 Auditing and Compliance with Good Manufacturing Practices (GMP)
9.5 Documentation and Record-Keeping in Bioprocess Operations
9.6 Continuous Improvement and Quality Management Systems in Bioprocessing
9.7 Conclusions and References
10: Operation and control of Submerged Liquid Fermenters
10.1 Introduction
10.2 Overview of Submerged Liquid Fermenters (SLFs)
10.3 Operational Parameters and Control Strategies in SLFs
10.4 Monitoring and Adjustment of Bioreactor Conditions
10.5 Strategies for Scaling Up SLF Processes
10.6 Case Studies and Troubleshooting in SLF Operation and Control
10.7 Conclusions and References
11: Operation and control of submerged solid fermenters
11.1 Introduction
11.2 Overview of Submerged Solid Fermenters (SSFs)
11.3 Operational Parameters and Control Strategies in SSFs
11.4 Monitoring and Adjustment of Bioreactor Conditions
11.5 Strategies for Scaling Up SSF Processes
11.6 Case Studies and Troubleshooting in SSF Operation and Control
11.7 Conclusions and References
12: Equipment for downstream Product Recovery and Purification
12.1 Introduction to Product Recovery and Purification Processes
12.2 Types of Equipment for Product Recovery
12.3 Equipment for Product Purification: Separation and Purification Techniques
12.4 Criteria for Equipment Selection in Recovery and Purification Processes
12.5 Case Studies and Examples of Equipment Selection for Product Recovery and Purification
12.6 Conclusions and References
13: Modern Reactors and Software Tools in Bioprocess Development
13.1 Introduction
13.2 Evolution of Bioreactors: From Conventional to Modern Reactors
13.3 Single-Use Reactors: Advantages and Applications
13.4 Variable Volume Reactors: Benefits and Design Considerations
13.5 Software Tools for Bioprocess Development and Optimization
13.6 Integration of Modern Reactors and Software Tools: Case Studies and Future Trends
13.7 Conclusions and References
PART C: Bioreactor Scale up aspects and case studies
14: Scale-Up Rules and Reactor Accessories
14.1 Introduction to Scale-Up in Bioprocess Engineering
14.2 Principles and Considerations for Scale-Up
14.3 Rules and Guidelines for Successful Scale-Up
14.4 Reactor Accessories and Upgrades for Large-Scale Operations
14.5 Calculation of Scale-Up Parameters and Scaling Factors
14.6 Conclusions and References
15: Scale-Up Strategies for Submerged Liquid Fermentation
15.1 Overview of Scale-Up in SLF Processes
15.2 Scale-Up Considerations and Challenges in SLF
15.3 Strategies for Lab-Scale to Large-Scale SLF
15.4 Calculation of Key Parameters in SLF Scale-Up
15.5 Case Studies and Examples of SLF Scale-Up with Detailed Calculations
15.6 Conclusions and References
16: Scale-Up Strategies for Solid State Fermentation
16.1 Overview of Scale-Up in SSF Processes
16.2 Scale-Up Considerations and Challenges in SSF
16.3 Strategies for Lab-Scale to Large-Scale SSF
16.4 Calculation of Key Parameters in SSF Scale-Up
16.5 Case Studies and Examples of SSF Scale-Up with Detailed Calculations
16.6 Conclusions and References
17: Scale-Up Constraints from Lab Scale to Industrial Scale
17.1 Limitations and Challenges in Scaling Up from Lab to Industrial Scale
17.2 Factors Affecting Scale-Up Constraints in Bioprocesses
17.3 Process Validation and Optimization for Industrial-Scale Operations
17.4 Strategies to Address Scale-Up Constraints and Mitigate Risks
17.5 Case Studies and Lessons Learned in Lab-to-Industrial Scale-Up
17.6 Conclusions and References
PART D: Bioproducts
18: Commercial Bioproducts from Bioreactors
18.1 Introduction to Commercial Bioproducts
18.2 Biopharmaceuticals: Vaccines, Antibodies, and Therapeutic Proteins
18.3 Industrial Enzymes: Applications and Market Impact
18.4 Biofuels and Biochemicals: Production and Market Trends
18.5 Specialty Chemicals and Consumer Goods: Bioplastics and Biopolymers
18.6 Case Studies and Success Stories of Commercial Bioproducts
18.7 Conclusions and References
19: Bioproducts from Fermentation and their Economic Impact
19.1 Overview of Bioproducts from Fermentation Processes
19.2 Bioproducts in the Global Economy: Market Size and Growth
19.3 Economic Impact of Bioproducts in Various Industries
19.4 Sustainability and Green Bioproducts: Environmental Benefits
19.5 Future Prospects and Emerging Trends in Bioproduct Development
19.6 Conclusions and References
20: Manufacturing Techniques for Bioproducts
20.1 Introduction to Manufacturing Processes for Bioproducts
20.2 Upstream Processing: Fermentation and Cell Culturing
20.3 Downstream Processing: Separation, Purification, and Formulation
20.4 Process Optimization and Scale-Up in Bioproduct Manufacturing
20.5 Emerging Technologies and Innovative Manufacturing Approaches
20.6 Quality Control and Regulatory Considerations in Bioproduct Manufacturing
20.7 Conclusions and References
Product details
Product details
- Edition: 1
- Latest edition
- Published: August 26, 2025
- Language: English
About the editors
About the editors
VS
Vinayaka B. Shet
Dr. Vinayaka B Shet is an Associate Professor in the Department of Biotechnology Engineering, NMAMIT, off-campus centre, Nitte (Deemed to be university), Nitte, Karnataka, India. He holds a Ph.D. in Biotechnology from VTU Belgavi, an M.Tech. in Industrial Biotechnology from the National Institute of Technology Karnataka (NITK), and a B.E. in Biotechnology from P.A. College of Engineering, Mangaluru. He has over 18 years of experience in Academics, 10 years in Research, and 1 year in Industry. He received a young researcher award. He has published over 30 research articles and 10 book chapters and holds two Indian patents. He is a reviewer of various journals. He serves as Coordinator (Accreditation and Compliance) at Nitte (Deemed to be University), Mangaluru, India.
SK
Sandesh Kanthakere
NM
Nabisab Mujawar Mubarak
RK
Rama Rao Karri
Rama Rao Karri is an Associate Professor in the Faculty of Engineering at Universiti Teknologi Brunei. He holds a PhD from IIT Delhi and a Master’s from IIT Kanpur in Chemical Engineering. With over 20 years of experience in academia, industry, and research, he has experience working in multidisciplinary fields and has expertise in various evolutionary optimization techniques and process modelling. His expertise extends to process modeling and simulation, focusing on optimizing chemical and environmental processes to improve efficiency and sustainability. He serves on editorial boards of several international journals and acts as a peer reviewer for numerous reputed publications. He has held editorial roles including Editor-in-Chief and Associate Editor for journals such as Scientific Reports and the International Journal of Energy and Water Resources. He is also a guest editor for special issues on nanocomposites and environmental engineering topics. Additionally, he contributes to authoring and editing books on sustainable technologies, environmental remediation, and process monitoring with leading academic publishers. He also has the distinction of being listed in the Top 2% of the world’s most influential scientists in the area of environmental sciences and chemical engineering since 2021, compiled and published by Stanford University.
MH
Mohammad Hadi Dehghani
Prof. Dr. Mohammad Hadi Dehghani is a full professor at the Tehran University of Medical Sciences (TUMS), School of Public Health, Department of Environmental Health Engineering, Tehran, Islamic Republic of Iran. His scientific research interests include environmental science. He is the author of various research studies published in national and international journals and conference proceedings and head of several research projects at the TUMS. He has authored 20 books, 26 book chapters and more than 270 full papers published in peer-reviewed journals. He is an editorial board member, guest editor, and reviewer in many internal and international journals and a member of several international science committees worldwide. He also has the distinction of being listed in the top 2% of the world’s most influential scientists in the area of environmental sciences. He is a supervisor and advisor for many PhD and MSc theses at the TUMS. He is currently also a member of the Iranian Association of Environmental Health (IAEH) and member of the Institute for Environmental Research (IER) at the TUMS. He is an editor for 14 edited books (English): (1) Soft Computing Techniques in Solid Waste and Wastewater Management, (2) Environmental and Health Management of Novel Coronavirus Disease (COVID-19), (3) Green Technologies for the Defluoridation of Water, (4) Pesticide Remediation Technologies From Water and Wastewater, (5) COVID-19 and Sustainable Development Goals, (6) Industrial Wastewater Treatment: Emerging Technologies for Sustainability, (7) Sustainable Materials for Sensing and Remediation of Noxious Pollutants, (8) Wastewater-Based Epidemiology for the Assessment of Human Exposure to Environmental Pollutants, (9) Water Treatment Using Engineered Carbon Nanotubes, (10) Water, the Environment and the Sustainable Development Goals, (11) Sustainable Remediation Technologies for Emerging Pollutants in Aqueous Environment, (12) Health and Environmental Effects of Ambient Air Pollution, (13) Health Effects of Indoor Air Pollution, (14) Diseases Attributed to Air Pollution.