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Biophysical Approaches for the Study of Membrane Structure Part A
- 1st Edition, Volume 700 - July 4, 2024
- Editors: Tobias Baumgart, Anna Maria Pyle, David Christianson, Markus Deserno
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 3 - 2 9 3 0 4 - 7
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 2 9 3 0 5 - 4
Biophysical Approaches for the Study of Membrane Structure, Part A, Volume 700 explores lipid membrane asymmetry and lateral heterogeneity. A burst of recent research has shown… Read more
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Request a sales quoteOther sections cover Using the yeast vacuole as a system to test the lipid drivers of membrane heterogeneity in living cells, Direct quantification of cellular membrane lipids using ratiometric fluorescence sensors, The spectral phasor approach to resolving membrane order with environmentally sensitive dyes, The use of hemifusion to create asymmetric giant unilamellar vesicles: Insights on induced order domains, Advanced microscopy methods to study membrane pores, Use of cryo-EM to study membrane phase separation, and much more.
- Explore the state-of-the-art of lipid membrane asymmetry
- Covers experimental, theoretical, and computational techniques to create and characterize asymmetric lipid membranes
- Teaches how these kinds of approaches create and characterize laterally inhomogeneous membranes
- Cover image
- Title page
- Table of Contents
- Series Page
- Copyright
- Contributors
- Preface
- Chapter One: Evaluation of functional transbilayer coupling in live cells by controlled lipid exchange and imaging fluorescence correlation spectroscopy
- Abstract
- 1 Arena
- 2 Before you begin
- 3 Key resources table
- 4 Equipment
- 5 Step-by-step method details
- 6 Expected outcomes
- 7 Advantages
- 8 Limitations and possible improvements
- 9 Optimization and troubleshooting
- 10 Safety considerations and standards
- 11 Connections
- Acknowledgements
- References
- Chapter Two: Fluorescence imaging of lamellipodin-mediated biomolecular condensates on solid supported lipid bilayer membranes
- Abstract
- 1 Physical situation
- 2 Challenges
- 3 Typical solution(s)
- 4 The big idea
- 5 Materials and equipment
- 6 Method details
- 7 Expected outcomes
- 8 Quantification and statistical analysis
- 9 Advantages
- 10 Limitations
- 11 Optimization and troubleshooting
- 12 Safety considerations and standards
- 13 Alternative methods/procedures
- 14 Connections
- References
- Chapter Three: The effect of hydrostatic pressure on lipid membrane lateral structure
- Abstract
- 1 Arena
- 2 High pressure small angle x-ray scattering and microscopy
- 3 Before you begin
- 4 Example experiment: Triggering lateral phase separation in ternary model membranes
- 5 Materials and equipment
- 6 Step-by-step method details
- 7 Analysis
- 8 Outcomes and analysis
- 9 Advantages
- 10 Safety considerations and standards
- 11 Connections
- References
- Chapter Four: Using the yeast vacuole as a system to test the lipidic drivers of membrane heterogeneity in living cells
- Abstract
- 1 Arena
- 2 Method 1: Imaging of yeast vacuole domains
- 3 Method 2: Biochemical isolation of yeast vacuoles
- 4 Method 3: µ-lipophagy or lipid droplet flux assay for vacuole domain function
- 5 Connections
- References
- Chapter Five: The spectral phasor approach to resolving membrane order with environmentally sensitive dyes
- Abstract
- 1 Introduction
- 2 Preparing giant unilamellar vesicles for hyperspectral imaging measurements
- 3 Measuring hyperspectral images of giant unilamellar vesicles
- 4 Using phasor analysis for hyperspectral imaging
- 5 Summary and conclusions
- 6 Connections
- References
- Chapter Six: The use of hemifusion to create asymmetric giant unilamellar vesicles: Insights on induced order domains
- Abstract
- 1 Arena
- 2 Challenges
- 3 Typical solutions
- 4 Common issues
- 5 Method to prepare asymmetric giant unilamellar vesicles using hemifusion
- 6 Expected outcomes
- 7 Quantification and statistical analysis
- 8 Advantages
- 9 Limitations
- 10 Optimization and troubleshooting
- 11 Safety considerations
- 12 Alternative methods/procedures
- Acknowledgement
- References
- Chapter Seven: Super-resolution microscopy methods to study membrane pores in situ
- 1 Physical situation
- 2 Before you begin
- 3 Materials and equipment
- 4 Dual-color STED microscopy of BAK and BAX
- 5 STORM microscopy of BAK-induced pores in apoptotic mitochondria in situ
- 6 Expected outcomes
- 7 Quantification and statistical analysis
- 8 Advantages
- 9 Limitations
- 10 Optimization and troubleshooting
- 11 Optimization and troubleshooting of STED microscopy
- 12 Connections
- References
- Chapter Eight: Phase separation in model lipid membranes investigated with cryogenic electron microscopy
- Abstract
- 1 ARENA
- 2 Cryo-EM imaging of liposomes
- 3 Before you begin
- 4 Key resources table
- 5 Materials and equipment
- 6 Step-by-step method details
- 7 Expected outcomes
- 8 Quantification and statistical analysis
- 9 Advantages
- 10 Limitations
- Acknowledgements
- References
- Chapter Nine: Using lipid binding proteins and advanced microscopy to study lipid domains
- Abstract
- Abbreviations
- 1 Introduction
- 2 Visualizing distribution of SM-rich and/or Chol-rich lipid domains in the presence of HIV-1 Gag by FLIM-FRET
- 3 Labeling intracellular SM-rich domains
- 4 Summary
- Acknowledgements
- References
- Chapter Ten: Structural characterization of lateral phase separation in polymer–lipid hybrid membranes
- Highlights
- Abstract
- 1 Introduction to intramembrane heterogeneity in polymer–lipid hybrid membranes
- 2 Application of atomic force microscopy in analyzing hybrid polymer–lipid membranes
- 3 Characterization of hybrid polymer–lipid membranes using X-ray scattering techniques
- 4 Investigation of hybrid polymer–lipid membranes through cryo-electron microscopy
- References
- Chapter Eleven: Applications of phase-separating multi-bilayers in protein-membrane domain interactions
- Abstract
- 1 Arena
- 2 Spin‐Coated Multi-bilayers
- 3 Before You Begin
- 4 Materials and Equipment
- 5 Alternatives
- 6 Step‐By‐Step Method Details
- 7 Notes
- 8 Expected Outcomes and Analysis
- 9 Optimization and Troubleshooting
- References
- Chapter Twelve: Studying lipid flip-flop in asymmetric liposomes using 1H NMR and TR-SANS
- Abstract
- 1 Introduction
- 2 Asymmetric vesicle preparation by cyclodextrin-mediated lipid exchange
- 3 Lipid flip-flop analysis using solution 1H NMR
- 4 Lipid flip-flop analysis of asymmetric liposomes using TR-SANS
- 5 Conclusions and connections
- Acknowledgements
- References
- Chapter Thirteen: Thickness determination of hydroperoxidized lipid bilayers from medium-resolution cryo-TEM images
- Abstract
- 1 Arena
- 2 Membrane profile improvement by selective radial alignement
- 3 Before you begin
- 4 Materials and equipment
- 5 Step-by-step method details
- 6 Expected outcomes
- 7 Conclusions and connections
- References
- Chapter Fourteen: Structure of symmetric and asymmetric lipid membranes from joint SAXS/SANS
- Abstract
- 1 Arena
- 2 Small angle scattering
- 3 Before you begin
- 4 Materials and sample preparation procedures
- 5 Expected outcomes
- 6 Quantification and data analysis
- 7 Advantages and limitations
- 8 Safety considerations and standards
- 9 Alternative methods/procedures
- 10 Connections
- References
- Chapter Fifteen: Quantification of membrane geometry and protein sorting on cell membrane protrusions using fluorescence microscopy
- Abstract
- 1 Arena
- 2 “The method”
- 3 Before you begin
- 4 Key resources table
- 5 Materials and equipment
- 6 Step-by-step method details
- 7 Expected outcomes
- 8 Quantification and statistical analysis
- 9 Safety considerations and standards
- 10 Potential connections
- Acknowledgments
- References
- Chapter Sixteen: A live cell imaging-based assay for tracking particle uptake by clathrin-mediated endocytosis
- Abstract
- 1 Arena
- 2 Method for high throughput tracking of individual particles during cellular uptake via clathrin-mediated endocytosis
- 3 Before you begin
- 4 Key resources table
- 5 Equipment
- 6 Step-by-step method details
- 7 Expected outcomes
- 8 Quantification and statistical analysis
- 9 Advantages
- 10 Limitations
- 11 Optimization and troubleshooting
- 12 Safety considerations and standards
- 13 Connections
- References
- Chapter Seventeen: Pore-spanning membranes as a tool to investigate lateral lipid membrane heterogeneity
- Abstract
- 1 Introduction
- 2 General aspects of phase-separated pore-spanning membranes
- 3 Preparation of phase-separated pore-spanning membranes
- 4 Quantification and statistical analysis
- 5 Summary and conclusions
- References
- Chapter Eighteen: MαCD-based plasma membrane outer leaflet lipid exchange in mammalian cells to study insulin receptor activity
- Abstract
- 1 Arena
- 2 MαCD mediated lipid exchange method
- 3 Before you begin
- 4 Key resources table
- 5 Materials and equipment
- 6 Step by step method details
- 7 Expected outcomes
- 8 Quantification and statistical analysis
- 9 Advantages
- 10 Limitations
- 11 Optimization and troubleshooting
- 12 Alternative methods/procedures
- 13 Connections
- References
- No. of pages: 534
- Language: English
- Edition: 1
- Volume: 700
- Published: July 4, 2024
- Imprint: Academic Press
- Hardback ISBN: 9780443293047
- eBook ISBN: 9780443293054
TB
Tobias Baumgart
DC
David Christianson
After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.
MD