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Bioconjugate Techniques

Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provid… Read more

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Description

Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.

Key features

  • A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab
  • Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates
  • More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates
  • Includes entirely new chapters on the latest areas in the field of bioconjugation as follows:
    Microparticles and nanoparticles
    Silane coupling agents
    Dendrimers and dendrons
    Chemoselective ligation
    Quantum dots
    Lanthanide chelates
    Cyanine dyes
    Discrete PEG compounds
    Buckyballs,fullerenes, and carbon nanotubes
    Mass tags and isotope tags
    Bioconjugation in the study of protein interactions

Readership

Researchers in biochemistry, molecular biology, immunology, pharmacology, cell biology, and microbiology

Table of contents

PART I Bioconjugate Chemistry

1. Functional Targets

2. The Chemistry of Reactive Groups

PART II Bioconjugate Reagents

3. Zero-Length Cross-Linkers

4. Homobifunctional Cross-linkers

5. Heterobifunctional Cross-linkers

6. Trifunctional Cross-linkers

7. Dendrimers and Dendrons

8. Cleavable Reagent Systems

9. Fluorescent Probes

10. Bifunctional Chelating Agents and Radioimmunoconjugates

11. Biotinylation Reagents

12. Iodination Reagents

13. Silane Coupling Agents

14. Microparticles and Nanoparticles

15. Buckyballs, Fullerenes, and Carbon Nanotubes

16. Mass Tags and Isotope Tags

17. Chemoselective Ligation; Bioorthogonal Reagents

18. Discrete PEG Compounds

PART III Bioconjugate Applications

19. Preparation of Hapten - Carrier Immunogen Conjugates

20. Antibody Modification and Conjugation

21. Immunotoxin Conjugation Techniques

22. Preparation of Liposome Conjugates and Derivatives

23. Avidin - Biotin Systems

24. Preparation of Colloidal-Gold-Labeled Proteins

25. Modification with Synthetic Polymers

26. Enzyme Modification and Conjugation

27. Nucleic Acid and Oligonucleotide Modification and Conjugation

28. Bioconjugation for the Study of Protein Interactions

Product details

About the author

GH

Greg T. Hermanson

Affiliations and expertise
Director of Technology, Thermo Fisher Scientific, Rockford, IL USA

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