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Bioconjugate Techniques
- 2nd Edition - July 26, 2010
- Author: Greg T. Hermanson
- Language: English
- eBook ISBN:9 7 8 - 0 - 0 8 - 0 5 6 8 7 2 - 0
Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provid… Read more
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Request a sales quoteBioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.
- A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab
- Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates
- More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates
- Includes entirely new chapters on the latest areas in the field of bioconjugation as follows:
Microparticles and nanoparticles
Silane coupling agents
Dendrimers and dendrons
Chemoselective ligation
Quantum dots
Lanthanide chelates
Cyanine dyes
Discrete PEG compounds
Buckyballs,fullerenes, and carbon nanotubes
Mass tags and isotope tags
Bioconjugation in the study of protein interactions
Researchers in biochemistry, molecular biology, immunology, pharmacology, cell biology, and microbiology
PART I Bioconjugate Chemistry
1. Functional Targets
2. The Chemistry of Reactive Groups
PART II Bioconjugate Reagents
3. Zero-Length Cross-Linkers
4. Homobifunctional Cross-linkers
5. Heterobifunctional Cross-linkers
6. Trifunctional Cross-linkers
7. Dendrimers and Dendrons
8. Cleavable Reagent Systems
9. Fluorescent Probes
10. Bifunctional Chelating Agents and Radioimmunoconjugates
11. Biotinylation Reagents
12. Iodination Reagents
13. Silane Coupling Agents
14. Microparticles and Nanoparticles
15. Buckyballs, Fullerenes, and Carbon Nanotubes
16. Mass Tags and Isotope Tags
17. Chemoselective Ligation; Bioorthogonal Reagents
18. Discrete PEG Compounds
PART III Bioconjugate Applications
19. Preparation of Hapten - Carrier Immunogen Conjugates
20. Antibody Modification and Conjugation
21. Immunotoxin Conjugation Techniques
22. Preparation of Liposome Conjugates and Derivatives
23. Avidin - Biotin Systems
24. Preparation of Colloidal-Gold-Labeled Proteins
25. Modification with Synthetic Polymers
26. Enzyme Modification and Conjugation
27. Nucleic Acid and Oligonucleotide Modification and Conjugation
28. Bioconjugation for the Study of Protein Interactions
- No. of pages: 1323
- Language: English
- Edition: 2
- Published: July 26, 2010
- Imprint: Academic Press
- eBook ISBN: 9780080568720
GH
Greg T. Hermanson
Affiliations and expertise
Director of Technology, Thermo Fisher Scientific, Rockford, IL USARead Bioconjugate Techniques on ScienceDirect