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Biochemical Applications of Raman and Resonance Raman Spectroscopes
- 1st Edition - November 14, 2012
- Author: P Carey
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 4 3 3 5 0 0 - 4
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 1 5 8 1 2 - 1
Biochemical Applications of Raman and Resonance Raman Spectroscopies focuses on the application of Raman and resonance Raman spectroscopies to biochemical problems. The book… Read more
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Request a sales quoteBiochemical Applications of Raman and Resonance Raman Spectroscopies focuses on the application of Raman and resonance Raman spectroscopies to biochemical problems. The book reviews biological systems and details the application of Raman spectroscopy to biological molecules such as proteins, nucleic acids, and lipids. It also looks at codevelopments of lasers, optics, and electronics that drive advances in experimental Raman spectroscopy, along with the important ramifications of these advances for biochemical applications. This volume is organized into eight chapters and begins with an overview of the theoretical and experimental aspects of Raman spectroscopy, including a very brief explanation of what Raman and resonance Raman spectroscopies are and a discussion of their advantages and disadvantages for biochemical studies. The explanation of the Raman and resonance Raman effects is taken up in more detail in the next chapter, which develops the concept of the vibrational motions of molecules by initially considering mechanical ""ball and spring"" models and goes on to use this concept to formulate a classical model for Raman scattering. The resonance Raman effect is then described by another model which emphasizes the discrete or quantized energy levels available to a molecule. The reader is also introduced to the experimental aspects of Raman spectroscopy and the application of Raman spectroscopy across the entire field of biochemistry. Each chapter contains an outline of the basic chemistry and biochemical nomenclature involved. This book will be of interest to chemists, biochemists, and spectroscopists, as well as graduate students and experienced research workers.
Preface
Chapter l. Introduction
I. Raman Scattering
II. The Appearance of a Raman Spectrum
III. Basic Units
IV. Raman, Resonance Raman, and Infrared Spectroscopies
V. Advantages and Disadvantages of Raman Spectroscopy for Biochemical Studies
Chapter 2. Principles of Raman Spectroscopy
I. Introduction
II. The Vibrations of a Diatomic Molecule from a Classical Standpoint
III. A Classical Model for Raman Scattering
IV. Comparison with Infrared Spectroscopy
V. A Quantum Mechanical Picture of Raman Scattering
VI. The Vibrations of a Polyatomic Molecule
VII. Polarization Properties of Raman Scattering
VIII. Raman Intensities
Chapter 3. Experimental Raman Spectroscopy
I. Basic Optics of the Raman Experiment
II. Sampling Techniques and Sample Problems
III. Sophisticated Techniques
Chapter 4. Protein Conformation from Raman and Resonance Raman Spectra
I. Protein Structure
II. Introduction to Raman and Resonance Raman Studies of Proteins
III. Raman Studies of Proteins
IV. Resonance Raman Studies of the Chromophores of the Polypeptide Chain Using Excitation below 300 nm
Chapter 5. Resonance Raman Studies of Natural, Protein-Bound Chromophores
I. Introduction
II. Heme Proteins
III. Vitamin B12 (Cobalamin)
IV. Chlorophylls
V. Carotenoids
VI. The Visual Pigments and Bacteriorhodopsin
VII. Flavin Nucleotides
VIII. Metalloproteins
Chapter 6. Resonance Raman Labels
I. Introduction
II. Drug-Protein Complexes and Other Enzyme-Inhibitor Systems
III. Antibody-Hapten Complexes
IV. Permanently Labeled Sites—Arsanilazocarboxypeptidase A
V. Enzyme-Substrate Reactions
VI. Nucleic Acids and Membranes
Chapter 7. Nucleic Acids and Nucleic Acid-Protein Complexes
I. The Structure of Nucleic Acids
II. Polynucleotide Structure from the Normal Raman Spectrum
III. Applications to RNA and DNA Structures
IV. Viruses and Other Nucleic Acid-Protein Complexes
V. Resonance Raman Studies of Nucleic Acids
Chapter 8. Lipids, Membranes, and Carbohydrates
I. Structures of Lipids and Membranes
II. Raman Spectra of Lipids and Membranes
III. Carbohydrates
Suggestions for Further Reading
References
Index
- No. of pages: 276
- Language: English
- Edition: 1
- Published: November 14, 2012
- Imprint: Academic Press
- Paperback ISBN: 9780124335004
- eBook ISBN: 9780323158121