Biocatalyst Immobilization
Foundations and Applications
- 1st Edition - November 12, 2022
- Editor: Maria Lujan Ferreira
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 9 1 3 1 7 - 1
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 1 3 7 7 - 5
Biocatalyst Immobilization: Foundations and Applications provides a comprehensive overview of biocatalytic immobilization processes, as well as methods for study, character… Read more
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Request a sales quoteBiocatalyst Immobilization: Foundations and Applications provides a comprehensive overview of biocatalytic immobilization processes, as well as methods for study, characterization and application. Early chapters discuss current progress in enzyme immobilization and methods for selecting and pretreating enzymes prior to immobilization, with an emphasis on navigating common challenges and employing enzyme supports and post immobilization treatments to impact enzymatic activity. Process-based chapters instruct on measuring and reporting on enzyme immobilization efficiency, protein final content, quantification of reaction products, and the use of nanomaterials to characterize immobilized enzymes.
Later chapters examine recent advances, including novel enzymatic reactors, multi-enzymatic biocatalysts, enzymatic biosensors, whole cell immobilization, the industrial application of immobilized enzymes, and perspectives on future trends.
- Provides a thorough overview of biocatalyst and enzyme immobilization for research and practical application
- Presents methods based content that instructs in enzyme immobilization pretreatment, enzyme supports, post immobilization treatments, measuring enzyme immobilization efficiency, quantification of reaction products, and whole cell immobilization
- Features chapter contributions from international leaders in the field
Researchers in Biochemistry, Chemical Biology, Enzymatic Catalysis, Materials, Nanotechnology, and Applied Biotechnology; Students
- Cover image
- Title page
- Table of Contents
- Copyright
- Dedication
- Contributors
- Preface
- Acknowledgments
- Chapter 1: The enzyme, the support, and the immobilization strategy: The key findings to a desirable biocatalyst
- Abstract
- 1.1: Introduction
- 1.2: Enzyme immobilization: Enzyme, support, or hybrid biocatalyst?
- 1.3: Immobilization methodologies and immobilization chemistry
- 1.4: Supports for enzyme immobilization
- 1.5: Enzyme engineering for enzyme immobilization
- 1.6: Improve enzyme performance: Just an immobilization question? The immobilized biocatalyst engineering (IBE) approach
- 1.7: Conclusions and perspectives
- References
- Chapter 2: Selection and modification of enzymes prior to immobilization
- Abstract
- 2.1: Introduction
- 2.2: Selection of enzymes for various applications
- 2.3: Enzyme modification technologies
- References
- Chapter 3: Enzyme immobilization approaches
- Abstract
- Acknowledgment
- 3.1: Introduction
- 3.2: Methods of carrier binding
- 3.3: Factors that should be taken into consideration before planning immobilization techniques
- 3.4: Immobilized enzyme bioanalytical potential
- 3.5: Advantages and disadvantages of immobilization
- 3.6: Some applications of immobilized enzymes
- 3.7: Conclusions
- References
- Further reading
- Chapter 4: Postimmobilization treatments before applications
- Abstract
- 4.1: Introduction
- 4.2: Enzyme immobilization on/in insoluble carriers
- 4.3: Enzyme immobilization in membrane reactors
- 4.4: Summary
- References
- Chapter 5: Support-free immobilization
- Abstract
- 5.1: Introduction
- 5.2: Enzyme immobilization
- 5.3: Preparation of cross-linked enzyme aggregate (CLEA)
- 5.4: Preparation of coimmobilization of enzymes in combi-CLEAs
- 5.5: Preparation of magnetic CLEA (m-CLEAs) and m-Combi-CLEAs
- 5.6: Cross-linked enzyme crystals (CLECs)
- 5.7: Future trends and conclusion
- References
- Chapter 6: Measuring and reporting enzyme's immobilization efficiency
- Abstract
- Acknowledgments
- 6.1: General topics about protein quantification in biocatalysts
- 6.2: Free and immobilized enzymes: Important issues in protein quantification methods
- 6.3: Strategies to avoid mistakes in protein quantification
- 6.4: Other considerations in enzyme immobilization: Unexpected sources of error
- 6.5: CLEAs: A challenge for protein quantification and free and immobilized enzymatic activity comparison
- 6.6: A constructive criticism: The need for systematization
- 6.7: Conclusions and recommendations
- References
- Chapter 7: Some recent innovations related to enzyme immobilization
- Abstract
- Acknowledgments
- Competing interests
- 7.1: Introduction
- 7.2: Selection and synthesis of novel support systems for enzyme immobilization
- 7.3: Methods of immobilization
- 7.4: In silico toolbox for tools and optimization of rational design for novel approaches of enzyme immobilization
- 7.5: Integration of enzyme immobilization and protein engineering
- 7.6: Conclusions
- References
- Chapter 8: Enzyme immobilization for use in nonconventional media
- Abstract
- Acknowledgments
- 8.1: Enzyme immobilization for nonconventional media: Why and for what
- 8.2: Impact of additives in the aqueous media, nonaqueous media, and multiphasic media: Bioimprinting, surfactants, polymers, polycations, polyanions, biodegradable polymers, sugars, and others
- 8.3: Ionic liquids: It is interesting but would it be rentable? Differences with other reaction media
- 8.4: Some considerations for reactions performed in nonconventional biphasic liquid systems
- 8.5: Don’t forget the supports: What happens in nonconventional media vs water?
- 8.6: The drawbacks of different additives in the nonconventional reaction media of immobilized enzymes: Adsorbents for products, reservoirs of reactants
- 8.7: Adequate drying of the immobilized enzyme: Is lyophilization the solution?
- 8.8: Enzyme leaching in nonconventional media
- 8.9: Achieving a high enzymatic activity in CLEAs and its measurement: An example of a complex task
- 8.10: Conclusions
- References
- Chapter 9: Assaying the biological activities of immobilized enzymes
- Abstract
- Acknowledgments
- 9.1: Assays of immobilized versus free enzymes in model reactions
- 9.2: Sampling methodology: Why is it so important?
- 9.3: Preparation of samples to quantify reaction media composition and sources of mistakes
- 9.4: The keys to adequate quantification in chromatographic methods, chemistry and engineering, together: The hydrolysis and synthesis of triglycerides with lipases as examples
- 9.5: The problem of lack of reproducibility among laboratories and possible causes and solutions
- 9.6: The importance of being careful with details
- 9.7: Leaching of enzyme in front of an aqueous medium or multiphasic polar-non polar media; are there solutions?
- References
- Chapter 10: Enzyme immobilization on nanomaterials and nanostructured supports
- Abstract
- 10.1: Introduction
- 10.2: Factors influencing metal-based nanobiocatalysis
- 10.3: Metal-based nanocarriers for bionanocatalysis
- 10.4: Different approaches of synthesis
- 10.5: Biomimetic method/green synthesis method
- 10.6: In vivo synthesis
- 10.7: Iron oxide nanocarrier supports
- 10.8: Silver nanoparticles
- 10.9: Other oxide nanoparticles
- 10.10: Characterization of metal-based nanocarriers
- 10.11: Applications of metallic nanobiocatalysts
- 10.12: Conclusion
- References
- Further reading
- Chapter 11: Oriented immobilization of biomolecules on small surfaces
- Abstract
- Acknowledgments
- 11.1: Introduction
- 11.2: Conventional immobilization techniques for biosensing molecules
- 11.3: Bio-nanocapsules (BNCs)
- 11.4: Conventional immobilization techniques for enzymes
- 11.5: Ideal immobilization techniques for enzymes
- Author contributions
- References
- Chapter 12: Affinity immobilization and affinity layers
- Abstract
- 12.1: Introduction
- 12.2: Immobilization of enzymes
- 12.3: Methodology: Immobilization of pectinase on alginate beads by affinity layering
- 12.4: Experimental problems during affinity immobilization
- 12.5: Conclusions
- References
- Chapter 13: New enzymatic reactor designs: From enzymatic batch to 3D microreactors and monoliths
- Abstract
- Acknowledgments
- 13.1: Introduction
- 13.2: Enzymatic reactor configurations
- 13.3: Mesoporous silicates
- 13.4: Silica monoliths
- 13.5: Nanoporous gold supports
- 13.6: Metal organic frameworks
- 13.7: Supports for enzyme immobilization
- 13.8: Concluding remarks
- References
- Chapter 14: Immobilization of multienzymes: Problems and solutions
- Abstract
- 14.1: Introduction
- 14.2: Research progress of multienzyme immobilization
- 14.3: Challenges and prospect
- 14.4: Conclusions
- References
- Chapter 15: Enzymatic biosensors
- Abstract
- Acknowledgments
- Conflict of interests
- 15.1: Introduction
- 15.2: Major constituents of enzymatic biosensors
- 15.3: Enzyme immobilization
- 15.4: Enzyme-based biosensors in food diagnostics
- 15.5: Biosensors based on enzyme-inhibition chemistry
- 15.6: Applications of enzymatic biosensors
- 15.7: Agriculture and food
- 15.8: Conclusions
- References
- Chapter 16: Industrial applications of immobilized enzymes: Food and other areas
- Abstract
- Acknowledgment
- 16.1: Introduction
- 16.2: Enzymes in food industry
- 16.3: Selected cases of immobilized enzymes in food industry
- 16.4: Enzymes in human and animal nutrition
- 16.5: Chemical industry
- 16.6: Enzymes for biorefinery
- 16.7: Enzymes for wastewater treatment in pulp and paper industry
- 16.8: Enzymes for fine chemical production
- 16.9: Market and scaling from laboratory to industry
- 16.10: Other fields
- 16.11: Future perspectives
- References
- Chapter 17: Future perspectives in enzyme immobilization
- Abstract
- Acknowledgment
- 17.1: Introduction
- 17.2: New opportunities in enzyme immobilization
- 17.3: Challenges
- 17.4: Conclusions
- References
- Index
- No. of pages: 458
- Language: English
- Edition: 1
- Published: November 12, 2022
- Imprint: Academic Press
- Paperback ISBN: 9780323913171
- eBook ISBN: 9780323913775
MF
Maria Lujan Ferreira
PhD in Chemistry María Luján Ferreira is a Principal Investigator at CONICET (NATIONAL COUNCIL OF SCIENTIFIC AND TECHNICAL RESEARCH) of Argentina. She has published more than 150 articles in peer reviewed, international journals, and has participated with presentations in more than 80 argentinian scientific events and more than 120 international scientific meetings. Dr. Ferreira has also directed three Assistant Investigators at CONICET.
Her research focuses on all practical aspects of enzyme immobilization (including applied biotechnology) and the details of the experimental design of enzymatic reactions: how to prepare immobilized biocatalysts and sampling in biocatalytic reactions ( from biphasic and multiphasic reaction media) avoiding mistakes and optimizing procedures. Her interests include the molecular modeling of biocatalysts. In more than 20 years of scientific career she has studied polymerization catalysts, heterogeneous metallic catalysts and recently also nanozymes and ionzymes applied in environmental remediation (of dyes and pharmaceuticals) using advanced oxidation processes.
Affiliations and expertise
Principal Investigator, CONICET (National Council of Scientific and Technical Research), PLAPIQUI-UNS-CONICET, CCT Bahía Blanca, Bahía Blanca, Buenos Aires, Argentina.Read Biocatalyst Immobilization on ScienceDirect