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Basic Life Science Methods

A Laboratory Manual for Students and Researchers

  • 1st Edition - September 11, 2022
  • Latest edition
  • Authors: Javeed Ahmad Tantray, Nighat Un Nissa, Rasy Fayaz Choh Wani, Sheikh Mansoor
  • Language: English

Basic Life Science Methods: A Laboratory Manual for Students and Researchers presents forty of the most executed life science assays. The authors use a consistent structure to cov… Read more

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Description

Basic Life Science Methods: A Laboratory Manual for Students and Researchers presents forty of the most executed life science assays. The authors use a consistent structure to cover the preparation, execution and analysis of data from each method. Assays include estimation of cholesterol fractions, C-Reactive Protein, Genomic DNA isolation, Agarose Gel Electrophoresis, RT-PCR, DNA solution preparation, how to design primers, and enzyme-linked immunosorbent assay (ELISA). This book provides a complete reference containing step-by-step instructions on how to run life science assays. Laboratory staff can also benefit of the book as a training resource.

Key features

  • Provides a practical resource on designing, executing and analyzing experiments and analytical procedures
  • Includes detailed and standardized coverage of basic research methods in the area
  • Presents step-by-step instructions on how to execute a large selection of life sciences experiments

Readership

Graduate students and early career researcher in life sciences. Life sciences laboratory staff members looking for resources to update their practices

Table of contents

1. Calculations of Molarity and Normality

2. Glassware washing and sterilization techniques

3. pH Meter: Its use and calibration

4. Principle and working of Autoclave

5. Principle and working of Centrifuge

6. Principles and working of Laminar

7. Estimation of Total cholesterol (Flegg 1972)

8. Estimation of Creatinine

9. Estimation of blood Urea

10. Estimation of blood glucose By GOD – POD method

11. Determination of Albumin, and Globulin in Plasma

12. Quantitative determination of Homocysteine

13. Estimation of serum bilirubin

14. Estimation of diagnostic enzyme ALT

15. Estimation of diagnostic enzyme AST

16. To Estimate Protein by Lowry’s Method

17. Estimation of Reducing sugar by using Dinitro Salicylic Acid (DNS) Method

18. To perform quantitative determination of total protein in Serum/plasma

19. Enzyme Activity Using Starch assay

20. To Estimate Protein by Bradford’s Assay

21. To Identify a Protein Sample Using SDS-PAGE

22. Genomic DNA isolation from whole blood

23. PCR for gene amplification

24. Agarose Gel Electrophoresis (AGE)

25. RNA Isolation from Human tissue

26. Real-time polymerase chain reaction (RT-qPCR)

27. Preparation of competent cells by CaCl2 treatment

28. RNA Isolation from Plant tissues

29. DNA isolation from the bacterial culture broth

30. DNA isolation from fungal Mycelium

31. Quantification of the DNA using spectrophotometer

32. Formaldehyde gel electrophoresis of the isolated RNA sample

33. Paper chromatography of different amino acid

34. Separation of plants pigments using Column Chromatography

35. Separation of plant pigment by Thin Layer Chromatography

36. Chromatin Immunoprecipitation (ChIP) Protocol

37. Types of Media

38. Preparation of Nutrient Agar Media

39. Serial Dilution of the Bacterial Cells

40. Spread Plate Method of The Bacterial Cells

41. Streak Plate Method

42. Identified Bacterial Colony in Culture Broth

43. Pour plate method for bacterial colony counting

44. Gram Staining of Bacteria

45. Casein hydrolysis test

46. Antibiotic resistance test by Agar Well Diffusion Method

Product details

  • Edition: 1
  • Latest edition
  • Published: September 11, 2022
  • Language: English

About the authors

JT

Javeed Ahmad Tantray

Dr. Javeed Ahmad Tantray’s current research focus is on Human cardiovascular diseases obtained his Ph. D from Osmania University in Hyderabad, India. Dr. Tantray has done Post Doc studies with the Department of Zoology, University of Kashmir, receiving funding from the UGC-CSIR. He published many National and International peer-reviewed research articles. Dr. Tantray has published in high impact factor journals such as the International Journal of Cardiology, and Saudi journal of biological science. He has guided 50, M.Sc / and graduate students projects. He has received Junior scientist awards both at national and international level. He received the Best Abstract Award at the 4th Asia Pacific CardioMetabolic Syndrome Congress held by the Korean Society of CardioMetabolic Syndrome,with an award amount of USD 500. Dr. Tantray has organized a national seminar on "Cancer Bio-markers" and a one-day national workshop on “Assembly and usage of Foldscope”. Dr. Tantray is currently an Assistant Professor in the Department of Zoology, School of life sciences central university of Kashmir (J&K).
Affiliations and expertise
Assistant Professor, Department of Zoology, Central University of Kashmir, India

NN

Nighat Un Nissa

Nighat Un Nissa has completed her M.Sc. and M.Phil. From Department of zoology, University of Kashmir. Ms. Nissa is currently pursuing her Ph.D. in Zoology and is working on Fish parasites and their impact on pollution. She has published her research work in National and International journals. She was awarded Gold Medal for poster presentation in Animal science congress held by Department of Zoology, University of Kashmir.
Affiliations and expertise
Assistant Professor, Department of Zoology, University of Kashmir, India

RW

Rasy Fayaz Choh Wani

Rasy Fayaz Choh Wani PhD has completed her M.Sc. degree from Kashmir University. Ms. Rasy is currently pursuing Ph.D. She has published her research work in many reputed journals. She has presented her different papers in different international and national conferences. She is currently on research topic “Isolation and characterization of pathogenic bacterial microflora associated with cultured Cyprinus carpio in Kashmir Valley”.
Affiliations and expertise
Research Scholar, Department of Zoology, University of Kashmir, India

SM

Sheikh Mansoor

Dr. Sheikh Mansoor Shafi currently serves as a postdoctoral researcher at the Phenomics Laboratory within Jeju National University in South Korea. His primary research focuses on high-throughput phenomics, remote sensing and precision agriculture. Prior to this role, he held positions as a Postdoc Research Associate at SKIMS Srinagar and as a Project Associate-I at CSIR-Indian Institute of Integrative Medicine Jammu (IIIM). Throughout his academic journey, Dr. Mansoor has had the opportunity to work in reputed laboratories, including the Transcriptomics Labs at SKUAST Kashmir and the Department of Biotechnology and Pathology at Central Institute of Temperate Horticulture (CITH) Rangreth Srinagar. His educational background comprises a BSc degree from SP College of Science (University of Kashmir), an MSc degree from HNBGU Uttarakhand, and a Ph.D. degree from Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu.

Affiliations and expertise
Postdoctoral Researcher, Phenomics Laboratory, Department of Plant Resources and Environment, Jeju National University, South Korea

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