Autophagy Processes and Mechanisms

1st Edition - September 14, 2023
Authors: Rohan Dhiman, Sujit K. Bhutia
Language: English
Paperback ISBN:
9 7 8 - 0 - 3 2 3 - 9 0 1 4 2 - 0
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 9 0 1 4 3 - 7

Autophagy Processes and Mechanisms details the process of autophagy and its significance in diseases and aging. It provides insights into autophagy mechanisms and processes to bro… Read more

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Autophagy Processes and Mechanisms details the process of autophagy and its significance in diseases and aging. It provides insights into autophagy mechanisms and processes to broaden our understanding. By collecting recent progress on several aspects of the autophagy process, it provides a more integrative perspective and serves as a resource that can influence future research initiatives in the field. This new book is appropriate for basic and applied researchers in cell biology, biologists and those working in the pharmaceutical sciences.

Cover image
Title page
Table of Contents
Copyright
Contributors
About the authors
Chapter 1. mTOR signaling and autophagy regulation
1. Introduction
2. Structure of mTORC1
3. mTOR signaling in ULK1 activation to induce autophagy
4. mTOR upstream signaling in autophagy
5. Modulators of mTORC1
6. Conclusion and future perspectives
Chapter 2. AMPK signaling in autophagy induction
1. Introduction
2. Structure of AMPK
3. AMPK-mediated regulation of autophagy
4. Regulation and mechanism of action of AMPK
5. Pharmacological modulators of AMPK
6. Conclusion and future perspectives
Chapter 3. Atg1/ULK1 complex in autophagy process and regulation
1. Introduction
2. The yeast Atg1 complex versus the mammalian ULK1 complex
3. Structural aspects and biochemistry of ULK1 complex components
4. Regulation of the ULK1 complex
5. ULK1 and selective autophagy
6. ULK1-independent autophagy
7. Small-molecule inhibition of the Atg1/ULK1 complex
8. Conclusion and future perspectives
Chapter 4. Beclin1-associated complex in autophagy and regulation
1. Introduction
2. Beclin1 location and structure
3. Beclin1-associated core complex
4. Major Beclin1 interacting proteins regulating autophagy
5. Transcriptional regulation of Beclin1 complex proteins affecting autophagy
6. Post-translational modifications of Beclin1 complex proteins affecting autophagy
7. Conclusion
Chapter 5. ATG ubiquitin-like conjugation systems in autophagy
1. Introduction
2. The ATG12 conjugation system
3. The ATG8–LC3 conjugation system
4. Functional outcome of the autophagy conjugation system
5. Conclusion and future perspectives
Chapter 6. ATG8 family protein, cargo selection, and autophagy
1. Introduction
2. Classification of ATG8 family proteins
3. Structure of ATG8 proteins
4. Functions of mammalian ATG8 proteins
5. Autophagy-independent function of ATG8/LC3/GABARAP family proteins
6. Conclusions
Chapter 7. Mechanism of autophagosome–lysosome fusion in autophagy
1. Introduction
2. Autophagosome biogenesis: Double-membrane vesicle
3. Lysosomes: The final target for autophagosome-enclosed cargo degradation
4. Autophagosome and lysosome fusion: Autolysosome
5. Factors involved in autophagosome and lysosome fusion
6. Autophagosome–lysosome fusion inhibitors
7. Conclusion and future perspectives
Chapter 8. Lysosomes in autophagy induction and cellular homeostasis
1. Introduction
2. Role of lysosomes in autophagy induction
3. Lysosomal components in the autophagy process
4. Role of lysosomes in the autophagy-associated signaling pathway for maintenance of cellular homeostasis
5. Lysosome-mediated cell death for cellular and organismal homeostasis
6. Conclusion and future perspectives
Chapter 9. Autophagic lysosome reformation: The beginning from the end
1. Introduction
2. Autophagy and lysosomal reformation pathways
3. Mechanism of autophagic lysosome reformation
4. Significance of autophagic lysosome reformation and cellular homeostasis
5. Conclusion and future perspectives
Chapter 10. Lipids in membrane formation for autophagosomes and autophagy induction
1. Introduction
2. Lipids: A molecular determinant for autophagosome membrane remodeling
3. Lipids: An essential requisite for autophagosome growth
4. Lipids: A critical modulator of autophagosome–lysosome fusion
5. Lipids: A driving force behind autophagic lysosome reformation
6. Conclusion and future perspectives
Chapter 11. Implications of autophagy in health, disease, and aging
1. Introduction
2. Autophagy in infectious diseases
3. Dual effects of autophagy in cancer
4. Role of autophagy in congenital disorders
5. Autophagy in neurodegenerative diseases
6. Autophagy and aging
7. Conclusion
Chapter 12. Detection and experimental analysis of autophagy and processes in mammalian cell culture
1. Introduction
2. Methods for the detection and study of autophagosomes
3. Monitoring and measuring autophagy flux and autophagic activity
4. Conclusion
Index

Rohan Dhiman

Rohan Dhiman is Associate Professor in the Department of Life Science at Rourkela, Odisha, India. He completed his Ph.D. at Institute of Microbial Technology, Chandigarh before moving to University of Texas Health Science Centre at Tyler for post-doctorate training. After four years there with virulent mycobacteria to study the role of NK cells, regulatory T-cells and monocyte heterogeneity in tuberculosis, he returned to India and joined as a Research Scientist in Translational Health Science and Technology Institute where he started studying different facets of pathways related to autophagy and post-translational modification regulated by mycobacterium in infected macrophages. After joining in current institute in 2014, he started his independent lab and currently focusing on screening some libraries w.r.t. autophagy induction ability of the molecules and Calcimycin is one of the compounds that his lab found through this approach. He has published 35 papers till now and co-authored different book chapters.

Affiliations and expertise

Associate Professor, Department of Life Science at Rourkela, Odisha, India

Sujit K. Bhutia

Sujit Kumar Bhutia is currently working as an Associate Professor at the Department of Life Science, National Institute of Technology in Rourkela, India. He received his doctorate in cell biology and cancer from the Indian Institute of Technology, Kharagpur, India in 2008, and subsequently pursued post-doctoral research on autophagy and cancer at Virginia Commonwealth University, USA. His research interests are focused on understanding the role of autophagy and autophagy-dependent cell death in cancer for the development of novel therapeutics. He has published more than 90 research articles in international peer-reviewed journals and authored or co-authored numerous books and book chapters. He is also serving as a referee for several international journals. Dr. Bhutia has more than 10 years of teaching experience in the areas of cell biology, cell signaling, cancer biology, and cell death.

Affiliations and expertise

Associate Professor, Department of Life Science, National Institute of Technology in Rourkela, India