Antioxidant Methods
A Guideline for Understanding and Determining Antioxidant Capacity
- 1st Edition - April 14, 2024
- Author: Francisco Avelino
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 3 - 2 1 5 9 1 - 9
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 2 1 5 9 0 - 2
Antioxidant Methods: A Guideline for Understanding and Determining AntioxidantCapacity summarizes the importance of antioxidants as a class of compounds withinnume… Read more
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Capacity summarizes the importance of antioxidants as a class of compounds within
numerous areas of science and technology. The chapters elucidate diverse methodologies
for assessing the antioxidant capacity of an antioxidant, while also exploring the correlation
between its structural attributes and overall performance. Chapters include descriptions of
protocols used to measure antioxidant capacity by different methods, highlighting experimental
steps, bottlenecks, expected outcomes, advantages, limitations, and more.
This is the perfect reference for biomedical science researchers looking for the right method
for determining antioxidant capacity through the structure and functionalities of the compound,
as well as the mechanisms involved in the antioxidant action.
Capacity summarizes the importance of antioxidants as a class of compounds within
numerous areas of science and technology. The chapters elucidate diverse methodologies
for assessing the antioxidant capacity of an antioxidant, while also exploring the correlation
between its structural attributes and overall performance. Chapters include descriptions of
protocols used to measure antioxidant capacity by different methods, highlighting experimental
steps, bottlenecks, expected outcomes, advantages, limitations, and more.
This is the perfect reference for biomedical science researchers looking for the right method
for determining antioxidant capacity through the structure and functionalities of the compound,
as well as the mechanisms involved in the antioxidant action.
- Elucidates the relationship between the structure and functionality of antioxidant activity
- Fully describes the mechanisms involved in each determination method
- Provides guidance on choosing the right method for your compound
Researchers in biomedical science, Researchers in pharmacology, chemistry, and engineering, Health science professionals in medicine
- Cover image
- Title page
- Table of Contents
- Copyright
- Dedication
- About the author
- Preface
- Section 1. Introduction to the antioxidants
- Chapter 1. History and classification
- 1.1. History
- 1.2. Classification
- Chapter 2. Traditional antioxidants
- 2.1. Introduction
- 2.2. Traditional (synthetic) antioxidants market
- 2.3. Applications
- Chapter 3. Alternative antioxidants (bio-based antioxidants)
- 3.1. Introduction
- 3.2. Natural antioxidants market
- 3.3. Applications
- Chapter 4. Definition and methods for determining antioxidant capacity
- 4.1. Definition
- 4.2. Methods for determining antioxidant capacity (antioxidant assays)
- Chapter 5. Mechanisms involved in the antioxidant action
- 5.1. Introduction
- 5.2. Types of mechanisms
- Section 2. Antioxidant capacity
- Chapter 6. 2,2-Diphenyl-1-picrylhydrazyl (DPPH).
- 6.1. Introduction
- 6.2. Principle of the method
- 6.3. Strengths and limitations
- 6.4. Evaluation of the kinetics in the DPPH. assay
- Chapter 7. ABTS.+
- 7.1. Introduction
- 7.2. Principle of the method
- 7.3. Strengths and limitations
- 7.4. Complementary information about ABTS.+ assay
- Chapter 8. H2O2
- 8.1. Introduction
- 8.2. Principle of the method
- 8.3. Strengths and limitations
- 8.4. Complementary information
- Chapter 9. Ferric reducing antioxidant power (FRAP)
- 9.1. Introduction
- 9.2. Principle of the method
- 9.3. Strengths and limitations
- 9.4. Final considerations
- Chapter 10. Parameters used to evaluate the antioxidant capacity (inhibition, MRI, and IC50)
- 10.1. Introduction
- 10.2. Inhibition and MRI: Definitions and methods of calculation
- 10.3. IC50: Definition, calculation, and interpretation
- Section 3. Protocols for determining antioxidant capacity
- Chapter 11. 2,2-Diphenyl-1-picrylhydrazyl
- 11.1. Before you begin
- 11.2. Key resources table
- 11.3. Materials and equipment
- 11.4. Step-by-step method details
- 11.5. Expected outcomes
- 11.6. Quantification and statistical analysis
- 11.7. Advantages
- 11.8. Limitations
- 11.9. Optimization and troubleshooting
- 11.10. Safety considerations and standards
- 11.11. Alternative methods/procedures
- Chapter 12. ABTS.+
- 12.1. Before you begin
- 12.2. Key resources table
- 12.3. Materials and equipment
- 12.4. Step-by-step method details
- 12.5. Expected outcomes
- 12.6. Quantification and statistical analysis
- 12.7. Advantages
- 12.8. Limitations
- 12.9. Optimization and troubleshooting
- 12.10. Safety considerations and standards
- 12.11. Alternative methods/procedures
- Chapter 13. H2O2
- 13.1. Before you begin
- 13.2. Key resources table
- 13.3. Materials and equipment
- 13.4. Step-by-step method details
- 13.5. Expected outcomes
- 13.6. Quantification and statistical analysis
- 13.7. Advantages
- 13.8. Limitations
- 13.9. Optimization and troubleshooting
- 13.10. Safety considerations and standards
- 13.11. Alternative methods/procedures
- Chapter 14. FRAP
- 14.1. Before you begin
- 14.2. Key resources table
- 14.3. Materials and equipment
- 14.4. Step-by-step method details
- 14.5. Expected outcomes
- 14.6. Quantification and statistical analysis
- 14.7. Advantages
- 14.8. Limitations
- 14.9. Optimization and troubleshooting
- 14.10. Safety considerations and standards
- 14.11. Alternative methods/procedures
- Index
- No. of pages: 242
- Language: English
- Edition: 1
- Published: April 14, 2024
- Imprint: Academic Press
- Hardback ISBN: 9780443215919
- eBook ISBN: 9780443215902
FA
Francisco Avelino
Francisco Avelino is Assistant Professor at Federal Institute of Ceará (IFCE) in the Physical
Chemistry subarea since 2019. He holds a Ph.D. in Chemistry from the Federal University of Ceará (2019), Master in Chemistry from Federal University of Ceara´ (2017), and Bachelor of Chemistry from Federal University of Ceará (2014) with a period at the University of Coimbra (2012—2013). His research focuses on materials chemistry, polymer chemistry, and solution chemistry, including biomass valorization, biorefinery, lignin chemistry, lignin valorization, fabrication of biocomposites, and the determination of thermodynamic, structural, and spectral properties of electrolyte and
nonelectrolyte solutions. He is also the founder and leader of Biopolymer and Advanced Materials Group (BioMat), Coordinator of Research and Extension of IFCE Campus Iguatu, and Research Fellow of FUNCAP (BPI fellowship) and CNPq (DT 2 fellowship).
Affiliations and expertise
Federal Institute of Education, Science and Technology of Ceara, BrazilRead Antioxidant Methods on ScienceDirect