Animal Models of Disease Part A

1st Edition, Volume 185 - March 29, 2024
Editors: Lorenzo Galluzzi, Fernando Aranda Vega, Aitziber Buque Martinez, Jose Manuel Bravo-San Pedro
Language: English
Hardback ISBN:
9 7 8 - 0 - 4 4 3 - 2 2 2 3 8 - 2
eBook ISBN:
9 7 8 - 0 - 4 4 3 - 2 2 2 3 9 - 9

Animal Models of Disease, Part A, Volume 185 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on a… Read more

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Animal Models of Disease, Part A, Volume 185 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including New mouse model to study aneurysm development, Mouse Model of Secondary Cystic Echinococcosis, Modelling childhood cancer in Drosophila, Analysis of immunohistomorphological changes in the colonic mucosa in a high-saturated fat and high-cholesterol fed streptozotocin/nicotinamide diabetic rat model, Establishment of an orthotopic Glioblastoma mouse model for preclinical studies, Lateral Fluid Percussion Injury as a Model for Traumatic Brain Injury, Ovarian and colorectal peritoneal carcinomatosis in mouse models, and more.

Other chapters cover Genetically engineered mouse model of hepatocellular carcinoma, Radiotherapy protocol in cancer mouse models, Using C. elegans as a model for neurodegenerative diseases: Methodology and evaluation, Methodology for the induction of myocardial infarction and cardiac function evaluation, Behavioral assessment of fine socio-sexual olfactory cues detection in a mouse model of neurodegeneration, Heat shock and thermotolerance in Caenorhabditis elegans: an overview of laboratory techniques, and Using the model cestode Taenia crassiceps for the study of cysticercosis.

Cover image
Title page
Table of Contents
Series Editor
Copyright
Contributors
Preface
Acknowledgments
Competing interests
References
Chapter One Heat shock and thermotolerance in Caenorhabditis elegans: An overview of laboratory techniques
Abstract
1 Introduction
2 Materials and methods
3 Technical considerations for thermotolerance assays
4 Thermotolerance assays
5 Concluding remarks
References
Further reading
Chapter Two Using the model cestode Taenia crassiceps for the study of cysticercosis
Abstract
1 Introduction
2 Materials
3 Experimental infection
4 Parasite culture
5 Concluding remarks
6 Notes
Acknowledgments
References
Chapter Three Modeling childhood cancer in Drosophila melanogaster
Abstract
1 Introduction
2 Materials
3 Methods
4 Concluding remarks
References
Chapter Four Establishment of an orthotopic glioblastoma mouse model for preclinical studies
Abstract
1 Introduction
2 Materials
3 Methods
4 Notes
5 Concluding remarks
Acknowledgments
References
Chapter Five Peritoneal carcinomatosis in mouse models
Abstract
1 Introduction
2 Materials
3 Procedure
4 Follow-up
5 Concluding remarks
6 Notes
Disclosures
References
Chapter Six Establishment of a murine hepatocellular carcinoma model by hydrodynamic injection and characterization of the immune tumor microenvironment
Abstract
1 Introduction
2 Materials
3 Protocol
4 Conclusions and outlook
References
Chapter Seven Radiotherapy protocols for mouse cancer model
Abstract
1 Introduction
2 Materials
3 Methods
4 Conclusion
5 Notes
Disclosures
References
Chapter Eight Mouse model of secondary cystic echinococcosis
Abstract
1 Introduction
2 Materials
3 Methods and procedures
4 Concluding remarks
Acknowledgments
References
Chapter Nine Assessment of social behavior and chemosensory cue detection in an animal model of neurodegeneration
Abstract
1 Introduction
2 Materials
3 Urine extraction
4 Olfactory tests using social cues
5 Limitations
6 Data analysis
7 Concluding remarks
References
Chapter Ten Methodology for the induction of myocardial infarction and cardiac function evaluation
Abstract
1 Introduction
2 Materials
3 Myocardial infarction induction
4 Cardiac function evaluation
5 Concluding remarks
Acknowledgments
References
Chapter Eleven Analysis of immunohistomorphological changes in the colonic mucosa in a high-saturated fat and high-cholesterol fed streptozotocin/nicotinamide diabetic rat model
Abstract
1 Introduction
2 Does type 2 diabetes mellitus start in the intestine?
3 Modeling type 2 diabetes mellitus progression
4 High-saturated fat and high-cholesterol fed streptozotocin/nicotinamide T2DM rat model (HSFHCD-STZ/NA-T2DM) and colonic dysfunction in T2DM
5 Experimental protocol of high-saturated-fat high-cholesterol fed streptozotocin-nicotinamide T2DM rat model (HSFHCD-NA/STZ-T2DM)
6 Histopathological analysis of colonic mucosa
7 Quantification of collagen fibers in colonic mucosa
8 Quantification of goblet cells number
9 Colonic mucosa barrier integrity: Quantification of tight junctions' protein levels
10 Remark conclusions
11 Notes
Acknowledgments
References
Chapter Twelve Lateral fluid percussion injury: A rat model of experimental traumatic brain injury
Abstract
1 Introduction
2 Materials
3 Methods
4 Severity assessment
5 Expected results
6 Limitations
7 Concluding remarks
Acknowledgments
References

Lorenzo Galluzzi

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

Affiliations and expertise

Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA

Fernando Aranda Vega

Fernando Aranda holds a BSc in Biology (2006) and Biochemistry (2007) from the University of Navarra. Then, he specialized in different strategies of Cancer Immunotherapy with a MSc in Biomedical Research (2008), and a PhD Degree (2012) from the University of Navarra (Pamplona) – Cima University of Navarra. More than 12 years in translational research focus on antitumor immune responses and Cancer Immunotherapy. Author of 64 publications indexed in PubMed in prestigious international journals, with h-index 30 and 4,296 cites (October 2022). He completed the Program of Sara Borrell (ISCIII) -competitive Postdoctoral contract- in the Group of Immune Receptors of the Innate and Adaptive System (IDIBAPS), Barcelona (2016-2018). Co-author of 1 invention patent: Composition based on the fibronectin domain A for the treatment of melanoma - WO/2011/101332. In 2012, Fernando Aranda obtained a Scientific Award, "Profesor Durantez" II Edición, for the best scientific article in Tumor Immunology by Fundación LAIR. Recently, Fernando Aranda awarded a competitive Research Fellow contract “Miguel Servet tipo I” by Instituto de Salud Carlos III, to continue his independent researcher career (IP) in cancer immunotherapy issues. Specifically, he is involved in Translational Immunotherapy of Peritoneal Carcinomatosis. Currently, Fernando Aranda leads a research group in cooperation with Dr. Pedro Berraondo.

Affiliations and expertise

Fernando Aranda Vega, CIMA Universidad de Navarra, Program of Immunology and Immunotherapy, Spain

Aitziber Buque Martinez

Aitziber Buqué is currently a Post-Doctoral Associate with the Galluzzi Lab, in the Department of Radiation Oncology at Weill Cornell Medical College (New York), where she investigates innate and acquired mechanisms of resistance to immunotherapy in HR+ breast cancer and radiotherapy as a means to overcome them. Prior to joining the Galluzzi Lab (2018), Aitziber was a Post-Doctoral Associate with the Kroemer Lab in the Cordeliers Research Center (Paris, France; 2014-2018), after receiving her M.Sc. in Bioinformatics and Computational Biology (2006) from the Complutense University (Madrid, Spain) and her Ph.D. in Biomedicine (2013) from the BioCruces Research Institute (Barakaldo, Spain). Aitziber has a long-standing interest in the immunological mechanisms controlling cancer progression and response to treatment.

Affiliations and expertise

Postdoctoral Associate in Radiation Oncology, Radiation Oncology, Weill Cornell Medical College, NY, USA

Jose Manuel Bravo-San Pedro

Jose Manuel Bravo-San Pedro is currently a researcher at the Department of Physiology of the Complutense University of Madrid thanks to a Ramon y Cajal contract grant. He got his Ph.D. in biochemistry, cellular biology and genetics from the University of Extremadura (Caceres, Spain) in 2011, and he did a post-doctoral stage in the laboratory of Prof. Guido Kroemer. His main research interests have always been linked to autophagy, addressing this cellular process associated with neurodegenerative diseases or cancer and recently obesity and specifically related to problems in the correct functioning of the cilium. He is co-inventor of two patents and co-author of 110 publications indexed in PubMed in prestigious international journals, with h-index 45 and 23768 cites (Dec 2022).

Affiliations and expertise

Departamento de Fisiología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid, Spain