Animal Cell Technology

Section 1. Chairman's Statement

Presence and Future of Animal Cell Technology

Section 2. Making and Selecting Recombinant Cell Lines

Introduction of DNA Into Mammalian Cells

Retrotargeting: Use of Defective Retroviral DNA Fragments to Improve Recombinant Protein Production in Mammalian Cells

Receptor-Mediated Gene Delivery into Mammalian Cells

Improvement of Cell Lines for Large Scale Mammalian Cell Culturing

Drosophila Cell Lines as Hosts for Recombinant Protein Expression

Immortalized Cell Lines for Virus Diagnosis

Selection Strategies for Highly Productive Recombinant Cell Lines

Section 3. Cell Line Characterization

Population Analysis of a Recombinant Chinese Hamster Ovary Cell Line

Expressing Recombinant Human Protein Cultured in the Presence and Absence of Methotrexate Selective Pressure

Genetic and Biochemical Analysis of a Murine Hybridoma in Long-Term Continuous Culture

DNA Fingerprinting as a Quality Control Marker for the Genetic Stability of Production Cells

Analysis of SV40 Early Region Expression in Immortalized Mouse Macrophage Cell Lines

Section 4. Complex Media formulations

Stimulation of Murine Hybridomas by Cytokines

The Use of 2-Hydroxy-2,4,6-Cycloheptarin-1-One (Tropolone) as a Replacemetn for Transferrin

Comparison of Long R3IGF-1 with Insulin in the Support of Cell Growth and Recombinant Protein Expression in CHO Cells

Development of a Hybridoma Cloning Medium

Performance of Gamma-Irradiated Fetal Bovine Serum in Cell Culture

Low Level FCS Adaptation and Media Development for the Culture of Two Hybridoma Cell Lines Producing Igg and IGM Monoclonal Antibodies

Improved Bioreactor Productivity and Manufacturing Efficiency Using Liquid Medium Concentrates

Cell Culture Raw Materials Screening by Calcein-AM Fluorescence Using a 96 Well Plate format

Importance of Extra-Cellular Matrix in the Long Term Anchorage Dependent Growth of Cell Line CHO K1

Section 5. Protein and or Serum Free Cultures

Development of Serum Free Media for Engineered NSO Cell Lines

Chloresterol: An Important Additive for Serum-Free Media?

Serum Free Ultraculture? Medium: An Alternative to the Instability Generated by Long-Term Cultivation of Mouse Hybridomas

Investigations of the TSH Antibody Producing Mouse Hybridoma Cell Line under Serum-Free Media Condition

Interferon Gamma Production in Laboratory Bioreactor by Lens Culinaris Lectin Stimulated Human Buffy Coat Using Protein Free Medium

Evaluation of CHO Cell Growth in a Protein-Free Environment

Inactivation of Serum Contaminants

Adaption of Mammalian Cells to Protein-Free Growth

Section 6. Ammonia/Glutamine and other Inhibitors

Ammonium Ions Inhibit Cell Growth by Intracellular formation of UDP-Activated Hexosamines

Influence of the PH on the Ammonia Sensitivity of a Murine Hybridoma Cell Line

The Influence of Ammonium on T-Pa Production from Chinese Hamster Ovary (CHO) Cells

Detoxification of Ammonium in Bioreactor Cultivations

Influence of Lactate and Ammonia on the Death Rate of Hybridoma

The Effect of Glutamine-Containing Dipeptides on the Growth and Productivity of a Hybridoma

Inhibitory Substance(S) Secreted in Cell Culture Media of Recombinant CHO and a Hybridoma Cell Line

Growth of Hybridoma Cells is Inhibited by Gangliosides

Section 7. Apoptosis and Cell Biology

Programmed to Die: Cell Death via Apoptosis

Cell Death (Apoptosis) in Cell Culture

Cell Death by Necrosis and Adoptosis during the Culture of Commercially Important Cell Lines

Spontaneous Apoptosis Contributes Negatively to the Regulation of Hybridoma Cell Viability

Metabolic Characteristics and Specific Requests of Hybridoma and CHO Cell Lines

Nature of Cell-Cell Interactions Between Recombinant CHO Cells Expressing Human Interferon Gamma

Section 8. Perfusion Cultures and Segregation Methods

High Density Insect Cell Culture for the Production of Recombinant Proteins with the Baculovirus Expression System

The Super-Spinner: A Mass Cell Culture Bioreactor for the CO2 Incubator

Continuous Suspension CHO Cell Culture

Progress in Animal Cell Perfusion Technology with Superspin

Production of a Recombinant Protein in a High Density Insect Cell Cytoflow Reactor

A Compact Gravitational Settling Device for Cell Retention

Conical Bioreactor with Internal Lamella Settler for Perfusion Culture of Suspension Cells

Lamellar Clarifier - A New Device for Animal Cell Retention in Perfusion Culture Systems

Perfusion Culture of Suspended CHO Cells Employing Inclined Sedimentation

Section 9. Fixed and Fluidized Beds

Collegenase Production by a Cell Line in Collagen Microspheres in a Fluidized-Bed Reactor and Its Modelization

Development of a Bioreactor Process for Recombinant Cell Lines

Growth and Metabolism of CHO-Cells in Porous Glass Carriers

Evolution of the Capacitance of Cytodex 3 Microbeads Suspended in Growth Medium

Section 10. Porous Microcarriers

High Density Culture Using Macroporous Microcarrier

Growth of Spodoptera Cells on Porous Microcarriers (Verax) Production of Recombinant Proteins

Protein Free Culture of R-CHO and Hybridoma Cells and Macroporous PolyporeR Carrier

Section 11. Hollow Fiber Bioreactors

A Novel Hepatocyte Entrapment, Hollow Fiber Bioreactor as a Bio-Artificial Liver

Pitfalls of Bioprocessing a Human Monoclonal Multireactive IGM Antibody

Hollow Fiber Cell Culture - A Proven Alternative

Section 12. Novel Bioreactors

Development of a New Membrane Reactor for Large Scale Mammalian Cell Culture

A Simple Magnetic Driven Mini-Reactor for Fluidized and Packed Beds

Animal Cell Cultivation in the NASA Rotating-Wall Vessel

Human Cancer and Primary Cell Culture in the New Hybrid Bioreactor System Tecnomouse

Analysis of a Long Term Hybridoma Culture in a New Minimized High Cell Density Bioreactor

Cell-Bubble Interactions During Aeration are Strongly Influenced by Surfactants in the Medium and Can be Minimized in the Newly Developed Bubble Bed Reactor

Micro-Bubble Sparging of Anchorage Dependent Animal Cell Cultures

Rationalization of the Design of High Density Perfusion Cultures for Suspended Animal Cells

Simulation of Hybridoma Growth and Monoclonal Antibody Production in a Homogenous Dialysis Bioreactor

Section 13. Bioreactor Comparisons

Comparison of Biorector Technologies for Efficient Production of Recombinant α-Amidating Enzyme

A Comparison of Animal Cell Growth Using Microcarriers and Suspended Natural Aggregates

Comparative Studies of Cell Propagation Systems for Production of Human Acetylcholinesterase by Recombinant Cells

Production of a Monoclonal Antibody with a Serum Dependant Hybridoma in Different Reactor Systems

Transient Expression with Cos Cells on Spinner Scale

Production of The HIV-1 Neutralizing Human Monoclonal Antibody 2F5: Stirred Tank versus Fluidized Bed Culture

Section 14. Novel Measurements and Assays

The CytosensorTM-Microphysiometer: Biological Applications of Living Cells on Silicon Sensor

Mass Spectroscopy Determination of Insect Cell Respiration Rates in Culture and Production Processes

Intracellular PH Evolution During Batch Cultures Using Flow Cytometry

Cell Cycle Analysis as a Tool for Control and Regulation of Mammalian Cell Cultures in Bioreactors

Use of MTT-Assay in Studies of the CHO: DG44 Cell Line

On-line-Measurement of Metabolites in Mammalian Cell Bioreactors by Means of Enzyme Electrodes and of the Number of Living and Dead Cells

Fully Automated Image Analysis of Cell Number, Viability and Morphology

Fast Quantification of Mouse IgG from Cell Culture Supernatants

On-Line Monitoring of Growth and Viral Infection of Vero Cells in Collagen Microspheres in a Fluidized-Bed Bioreactor

On-Line Glucose Control of Animal Cell Cultures in Fluidized Beds

On-Line Indirect Measurement of Oxygen Uptake Rate in Bench Scale Hybridoma Cultures

Software Sensors for the Monitoring of High-Density Hybridoma Perfusion Cultures

Application of an Industrial Disc Stack Centrifuge for the Separation of Hybridoma Cell as a First Step in Downstream Processing

Section 15. Physical Factors in Bioreactors

Dynamic Interfacial Tension and Theological Properties of Cell Culture Medium with Shear Protectant Additives

Mixing Phenomena in Industrial Bioreactors with Perfusion Spin Filters

Hydrodynamic Control of Suspended Natural Animal Cell Aggregates

Modeling and Prediction of Animal Cell Disruption in Simple Flows

Biosynthetic Responses and Scanning Electron Microscopic Studies of Murine Hybridomas Subjected to Hydrodynamic Stresses

Section 16. Bioreactor Operation Strategies

Fed-Batch Strategies for Mammalian Cell Cultures

Growth and Productivity of Animal Cells: A Contribution to Some Factors Influencing Both

Simultaneous Control of Growth and Productivity Using a Mutant CHO Cell Line

Factors Affecting Growth, Infection and Protein Yield in Baculovirus-Infected Insect Cells

Effects of the Feed Medium Glucose Concentration on Growth and Metabolism of Hybridomas in Chemostat Culture

Effects of Glucose on the Production of Recombinant Protein C in Mammalian Cell Culture

Substrate Level Control in Fed-Batch Hybridoma Cultures

Effect of Dissolved Oxygen on Monoclonal Antibody Production from Hybridoma Cultured in Haemodialysers

Metabolic Parameters of a Hybridoma Cell Line in Batch and Continuous Cultivation

Overproduction of Monoclonal Antibodies in High Salt Medium in the Presence of Butyrate

Growth Control of Production Processes with BHK Cells and Regulation of the Perfusion Rate by Monitoring the Intracellular Nucleotide Pools

Control of The Maximal Cell Density in a Membrane Perfusion Reactor

Continuous Medium Recycling In Pilot Scale with a Recombinant Animal Cell Culture for Antithrombin III Production

Optimization of Antibody Production in a Fluidized Bed Bioreactor

Monoclonal Antibodies Released from Viable Hybridoma Cells at Different Stages of Growth

High Cell Density in Insect Cell Cultures

Semi-Continuous Production with the Baculovirus Expression System

Long Term Cultures of BHK Suspended Aggregates

Section 17. Kinetics and Modeling

Batch Kinetic Data of Hybridoma Growth and Productivity as a Basis for Simulation of Antibody Production in Different Culture Systems

Modeling Insect Cell Cultures Infected with Recombinant Baculovirus

Kinetic Analysis of Hybridoma Growth and Monoclonal Antibody Production in Semicontinuous and Continuous Cultures with Respect to Batch Cultures

Process Development and Kinetic Analysis of a Protein-Free Hybridoma Perfusion System

Steady-State Analysis of Hybridomas in Chemostta Culture

Kinetics Studies of Fed-Batch Hybridoma Cultures: Effect of Various Feeding Compositions and Flow Rates

A General Two-Step Procedure for the Kinetic Modeling of Animal Cell Cultures

Mathematical Modeling of the Growth and Production Kinetics in Hybridoma Cultures

Section 18. Downstream Processing

Impact of Improved Chromatographic Media on Productivity and Process Design in Downstream Processing

Direct Capture of Recombinant Proteins from Animal Cell Culture Using a Fluidized Bed

Chromatographic Separation of Immunoglobins on a New "Tentacle"-Type "TA"-Phase: Fractogel EMD TA 650(S)

Validation of Chromatographic Procedures Used in the Fractionation of Plasma Derivatives

Section 19. Products from Animal Cells in Culture

Successful Products and Future Business Prospects

Production of Recombinant Factor VIII from Perfusion Cultures: I Large-Scale Fermentation

Production of Recombinant Factor VIII from Perfusion Cultures: II Large-Scale Purification

Advantages of High Cell Density In Vitro Cultures in Down Stream Processing of Monoclonal IGG Antibody for In Vivo Use

Production, Purification and Application of Flatfish Interferon

Monoclonal Antibody Production Strategy for Serum Dependent Clone

High Level Measles Virus N Protein Expression Using a Recombinant Baculovirus: Assembly of Nucleocapsid-Like Structures

Equimolar Expression of Two Protein Chains in Recombinant Mammalian Cells

Expression of Mammalian Protein Farnesyltransferase in a Baculovirus System

Levels of Troponin I and Myosin Heavy Chain in Differentiated Adult Rabbit Cardiac Myocytes

Expression of the D2 and D3dopamine Receptors in Insect Cells Using the Baculovirus System

Production and Characterization of Soluble Mouse and Human Interferon-γ Receptors (IFN-γr) from Insect Cells

Generation of DNA-Packaging Proteins by Overexpression in the Baculovirus/Insect Cell System

Metalloproteinases and TIMPs Production by Aids-Kaposi Sarcoma Derived Cells

Endothelial Cells and Their Infection with the Rickettsia Cowdria Rumination: Prospects for Vaccine Production

Section 20. Post Translational Modifications

Changes in the Glycosylation Pattern of Recombinant Proteins Effected by Defined Culture Conditions of BHK-21 Cells

Glycosylation Analysis of a Human Monoclonal IgG Antibody Derived from a Human-Mouse Heterihybridoma

Culture PH and Ammonia Affect Expression Rates and Glycosation of Recombinant Mouse Placental Lactogen Proteins by CHO Cells

Analysis of the Product Consistency: Independant of Process Parameters, rt-PA Shows a Stable Glycosylation Pattern

Galactosylation of Human/Mouse IGG Anti-NP Antibodies and Its Detection

Characterization of Carbohydrate Chain on Light Chain of Human Monoclonal Antibody

Comparison of the Posttranslational Modifications of Human Proteins Secreted from Different Recombinant Baculovirus Infected Insect Cell Lines

Characterization of Intra- and Extracellular Proteases in Recombinant Mammalian and Hybridoma Cells

Impact of Factors Secreted by Mammalian and Insect Cells on Human Monoclonal Antibody Integrity

BiP - A Chaperone Involved in Antibody formation

Section 21. Cells in Culture for Toxicology Studies

Cell Systems for In Vitro Toxicology

Hepatocytes: In Vitro Pharmacology, Toxicology and Drug Metabolism

Development of an Optimized In Vitro System for Measurement of Human Tumor Response to Cytotoxic Agents

Three Dimensional Cell Cultures for Toxicity Testing and for the Estimation of the Affinity of Cells to Biocompatible Materials

Stable Expression of the Bovine Adrenal 11β-Hydroxylase, a Cytochrome P450 Enzyme, in CHO Cells as Tool for Drug Characterization

Activation and Expansion of Human Cytotoxic T Lymphocytes in Hollow Fiber Bioreactors

Section 22. Product Safety and Consistency Testing

Significance of Contamination with Viruses of Cell Lines Used in the Production of Biological Medicinal Products

Residual In Vitro Activity of DNA in Biologicals Treated by Beta-Propiolacetone: Rabies Vaccines as Model

The Development of Appropriate Viral Models for the Validation of Viral Inactivation Procedures

Regulatory Affairs: Safety Testing for Gene Therapy Products

Product Quality Studies of Antithrombin III during Medium Recycling in Pilot Scale

Product Consistency During Long Term Fed-Batch Culture

The Application of Insect Cells for Biopharmaceutical Production: Implications for Safety Testing

Generation of Linear Epitope Specific Antibodies against the Human Recombinant Tissue-Plasminogen-Activator (Rt-Pa)

List of Participants

Author Index

Index