Anaerobic Bacteriology

1. Methods of Growing Anaerobes The Anaerobic Jar Other Methods of Obtaining an Oxygen-Free Atmosphere Pre-Reduced Anaerobically Sterilized Roll Tube Technique Anaerobic Cabinets and Glove Boxes Shake Cultures and Fluid Cultures Reducing Agents Appendix References2. Media Inoculation of Media Preparation of Media Some Medium Additives Cooked Meat Medium VL (Viande—Levure) Broth Reinforced Clostridial Medium Columbia Agar Base Fresh Blood Agar Heated Blood (Chocolate) Agar Benzidine Heated Blood Agar Egg Yolk Agar and Human Serum Agar Milk Medium and Litmus Milk Milk Agar Casein Agar Gelatin Agar Deoxyribonuclease Agar (DNAse Agar) Tributyrin Agar Concentrated (Firm) Agar Media for Sporulation of Cl. Perfringens Fermentation Media Bile Broth Hydrogen Sulphide Production Indole Production Nitrate Reduction Catalase Production Urea Medium The API Microsystem for Anaerobes Selective Agents Selective Media Media for Anaerobic Blood Culture Stock Cultures of Anaerobes References3. Examination and Identification of Anaerobes Morphology and Staining Colonial Appearance Cultural Characteristics Antibiotic Disc Resistance Growth Inhibition and Stimulation Antibiotic Susceptibility Testing of Anaerobes Animal Inoculation and Protection Tests Serological and Toxicological Aspects of Anaerobes Gas Liquid Chromatography References4. Characteristics of The Pathogenic and Related Clostridia Definition Classification Clostridium Tetani Clostridium Botulinum The Histotoxic Clostridia of Infected Wounds Clostridium Perfringens Clostridium Novyi Clostridium Septicum and Clostridium Chauvoei Clostridium Histolyticum Clostridium Tertium Clostridium Sporogenes Clostridium Bifermentans and Clostridium Sordellii Clostridium Fallax Clostridium Cochlearium Clostridium Paraputrificwn Clostridium Lentoputrescens Clostridium Cadaveris Clostridium Sphenoides Clostridium Carnis Clostridium Difficile Clostridium Subterminale Clostridium Barati References5. The Non-Clostridial Anaerobes Bacteroides Fusobacterium Actinomyces and Arachnia Eubacterium Propionibacterium The Anaerobic Cocci Veillonella Alcalescens and Veillonella Parvula Peptococcus Peptostreptococcus References6. Infections Due To Non-Clostridial Anaerobes Obstetrical and Gynaecological Infections Other Genitourinary Infections Infections Related to the Gastrointestinal Tract Infections of the Respiratory Tract Intracranial Suppurative Disease Bone and Joint Infections Superficial and Soft Tissue Infections Bacteraemia Endocarditis Treatment of Bacteroides and Related Infections Diagnosis of Non-Clostridial Anaerobic Infections Actinomycosis References7. Clostridial Infections Wound Infections Tetanus Gas Gangrene (Clostridial Myonecrosis) Uterine Gas Gangrenous Infections Botulism Cl Perfringens Food Poisoning Enteritis Necroticans (Necrotizing Jejunitis) Necrotizing Colitis Other Clostridial Infections ReferencesIndex