Advances in CRISPR/Cas and Related Technologies
- 1st Edition, Volume 178 - March 5, 2021
- Imprint: Academic Press
- Editor: Dipanjan Ghosh
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 8 2 1 5 9 0 - 6
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 2 1 5 9 1 - 3
Advances in CRISPR/Cas and related technologies, Volume 179, the latest release in this ongoing series, deals with a wide variety of research topics related to recent advanc… Read more
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Request a sales quoteAdvances in CRISPR/Cas and related technologies, Volume 179, the latest release in this ongoing series, deals with a wide variety of research topics related to recent advancement in the genome editing techniques. Associated chapters in this new release include Challenges for Therapeutic application of CRISPR Cas techniques, Mitochondrial DNA modification by CRISPR Cas System: Challenges and future direction, Trends in CRISPR Cas technology application in cancer, Modified CRISPR-Cas for next generation application, Application of CRISPR Cas in Synthetic Biology: Challenges and Scopes, History of CRISPR Cas system from bacterial Adaptive Immune System to research application, and more.
- Covers the Cas9 protein modification for reduced off-target effect
- Includes discussions on Cas9 utilization for Metabolic Engineering
- Provides information on the use of Cas9 for targeted delivery in therapeutic application
Genetic Engenderers, Synthetic Biologists, Medicine Practitioners
- Cover image
- Title page
- Table of Contents
- Copyright
- Contributors
- Preface
- Chapter One: The evolution and history of gene editing technologies
- Abstract
- 1: Introduction
- 2: Gene editing technologies
- 3: Applications of gene editing technologies
- 4: Drawbacks of gene editing technologies
- 5: Ethics of gene editing technologies
- 6: Conclusions
- Chapter Two: CRISPR-Cas9: A fascinating journey from bacterial immune system to human gene editing
- Abstract
- 1: Introduction
- 2: CRISPR-Cas9: An exciting journey from bacteria to human
- 3: Therapeutic applications of the CRISPR-Cas9: Technology for precise gene therapy
- 4: Therapeutic applications of the CRISPR-Cas9: Problems and challenges
- 5: Future research
- Chapter Three: Modification of Cas9, gRNA and PAM: Key to further regulate genome editing and its applications
- Abstract
- 1: Introduction
- 2: Cas9 engineering
- 3: Structural based re-designing of Cas9
- 4: Customizing PAM specificity by leveraging directed evolution
- 5: Guide RNA (gRNA) engineering
- 6: Conclusion
- Chapter Four: CRISPR/dCas system as the modulator of gene expression
- Abstract
- 1: Introduction
- 2: CRISPRi
- 3: CRISPRa
- 4: Applications
- 5: Epigenome engineering and CRISPRi/a
- 6: dCas9 toolboxes beyond CRISPRi/a
- 7: Conclusion
- Acknowledgment
- Chapter Five: CRISPR-Cas system, antibiotic resistance and virulence in bacteria: Through a common lens
- Abstract
- 1: A place to begin
- 2: Bacterial CRISPR-Cas system
- 3: Bacterial antimicrobial resistance
- 4: Interference of CRISPR-Cas with bacterial antimicrobial resistance
- 5: Association of CRISPR-Cas with bacterial virulence
- 6: Detection of CRISPR-Cas system in bacteria
- 7: Applications of CRISPR-Cas for controlling antimicrobial resistance and virulence
- 8: A place to reflect
- Chapter Six: Trends in CRISPR-Cas9 technology application in cancer
- Abstract
- 1: Introduction
- 2: Genome-wide CRISPR-Cas9 library screening in cancer cells
- 3: Potential clinical application of CRISPR in cancer therapy
- 4: Quality control and bioinformatics of CRISPR guide design
- 5: Perils of CRISPR-Cas technology
- 6: Conclusion and future perspective
- Acknowledgment
- Chapter Seven: Mitochondrial DNA modification by CRISPR/Cas system: Challenges and future direction
- Abstract
- 1: Introduction
- 2: Mitochondrial DNA modification in disease condition
- 3: CRISPR/Cas mechanism
- 4: Implementation of CRISPR/Cas in mitochondrial DNA modification
- 5: Future perspective
- Chapter Eight: Immune responses to CRISPR-Cas protein
- Abstract
- 1: Introduction
- 2: The overview of CRISPR-Cas9 technology
- 3: The structure of CRISPR-Cas9
- 4: Challenges associated with the implication of CRISPR-CAS9 technology
- 5: Immunogenicity of Cas9 proteins
- 6: Future direction and strategies to deal with immunogenicity
- 7: Conclusion
- Acknowledgment
- Chapter Nine: Application of CRISPR-Cas systems in neuroscience
- Abstract
- 1: A basic overview of the CRISPR-Cas system
- 2: Delivery and expression mechanisms in applications of CRISPR-Cas9 based disruption of genes in the mammalian CNS
- 3: Gene editing with CRISPR-Cas9 in neurons
- 4: The repurposed CRISPR-Cas9: Nuclease deficient-cas9 (dcas9) and neuroscience
- 5: RNA-targeting with CRISPR in the neuron
- 6: Perspectives and concluding remarks
- Index
- Edition: 1
- Volume: 178
- Published: March 5, 2021
- No. of pages (Hardback): 284
- No. of pages (eBook): 284
- Imprint: Academic Press
- Language: English
- Hardback ISBN: 9780128215906
- eBook ISBN: 9780128215913
DG
Dipanjan Ghosh
Dr. Dipanjan Ghosh has almost 19 years of research experience in the field of Molecular Biology, Cell Biology, Biochemistry, Immunology and genome editing. Presently Dr. Ghosh is working as Assistant Professor in the Department of Natural Products at National Institute of Pharmaceutical Education and Research (NIPER)-Kolkata. After getting MSc. in Biochemistry from University of Calcutta, Dr. Ghosh went to Indian Institute of Technology (IIT) Kharagpur where he did his MTech in Biotechnology and Bioprocess Engineering and then PhD in the field of Immunology, Cancer Biology Biochemistry. In his first postdoc at University of Texas Health Science Centre at Houston he worked in the field of catalytic enzyme. He then moved to UT MD Anderson Cancer Centre, Houston TX where he gained enough experience in field of genome editing using ZFN and CRISPR Cas systems. Dr. Ghosh received DST FAST TRACK YOUNG SCIENTIST GRANT from Department of Science and Technology, India. Presently he has initiated Synthetic Biology through metabolic bioengineering at his lab and acting as leading investigator of two major funded projects (one from DBT and another one from WBDBT) in the field of CRISPR Cas mediated genome editing.
Dr. Ghosh has enough experience, skill, knowledge and publication in the field of genome editing.
Affiliations and expertise
Assistant Professor, Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Kolkata, IndiaRead Advances in CRISPR/Cas and Related Technologies on ScienceDirect