Advances in Cell Culture

Contributors to Volume 3

Preface

Philip Rodney White

Contents of Previous Volumes

Protoplasts and Plant Viruses

I. Virus Infection of Plant Protoplasts

II. Virus Replication in Plant Protoplasts

III. Resistance to Virus Infection

IV. In Vitro Methods for Recovering Virus Resistance

V. Perspectives and Problems for Genetic Engineering

References

Applications of Flow Cytometry to Tissue Culture Systems

I. Introduction

II. What Is Flow Cytometry?

III. Cell Kinetic Analysis

IV. Chromosome Analysis

V. Cell Marker Analysis

VI. Cell Functional Analysis

VII. Detection and Cloning of Mutant and Hybrid Cells

VIII. Conclusions

References

Plant Tissue Culture Systems for Screening of Plant Growth Regulators: Hormones, Herbicides, and Natural Phytotoxins

I. Introduction: General Considerations

II. Screening for Hormonal Activities

III. Screening for Yield Enhancement

IV. Screening Phytotoxic Compounds

V. Drift, Instability, Habituation, Phenotypic Adaptations, and Other Problems

VI. Concluding Remarks

VII. Appendix: Nomenclature of Growth Regulators

References

Microsporida in Cell Culture

I. Introduction

II. A Description of the Microsporida

III. Establishing Microsporida in Cell Cultures

IV. Observations of Microsporida in Cell Cultures

V. Other Techniques

VI. Uses of Microsporida in Cell Cultures

VII. Conclusion

References

Growth Factors and Hemopoietic Cell Differentiation

I. Blood Cell Diversity

II. Colony Stimulating Factors: Nomenclature

III. Stem Cell Heterogeneity

IV. Production of Myeloid Cells M-CFC, G-CFC, and GM-CFC

V. Erythroid Cells

VI. Megakaryocyte and Eosinophil Production

VII. Production of Lymphocytes

VIII. Leukemic Cells

IX. Growth Factors

X. Growth Factors for Multipotential Stem Cells

XI. GM-CSF, M-CSF, and G-CSF

XII. Erythropoietic Stimuli

XIII. MEG-CSF and EO-CSF

References

Mosquito Cell Culture

I. Introduction

II. Cell Lines

III. Growth Requirements

IV. Characteristics

V. Clonal Analyses

VI. Conclusions

References

The Coming of Age of WI-38

I. The Birth of WI-38

II. Distribution of WI-38

III. Who Owns WI-38?

IV. The Mortality of Cultured Normal Cells

V. The Great Virus Vaccine Substrate Debate

VI. Advances in Cytogerontology

References

Index