Advances in Cell Culture

1st Edition, Volume 2 - September 23, 2014
Editor: Karl Maramorosch
Language: English
Paperback ISBN:
9 7 8 - 1 - 4 8 3 2 - 3 4 7 9 - 3
eBook ISBN:
9 7 8 - 1 - 4 8 3 2 - 6 8 1 9 - 4

Advances in Cell Culture, Volume 2 is a compendium of critical reviews in the field of cell culture. The chapters in the book are prepared by recognized authorities in their… Read more

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Advances in Cell Culture, Volume 2 is a compendium of critical reviews in the field of cell culture. The chapters in the book are prepared by recognized authorities in their specialized fields. The text deals with all the aspects of cell culture, which includes the growth of individual cells or cell populations, the growth of small fragments of explanted tissue, the growth of organs, and the growth of obligate parasites in cell culture systems. This volume provides reviews of important aspects of in vitro cultivation and presents the increasing understanding of the wide ramifications of in vitro techniques. The book also focuses on specific topics in cell culture such as cellular senescence in vitro; introduction and expression of foreign DNA sequences in mammalian cells; media for insect cell cultures; agricultural applications of in vitro plant cultivation; and fish cell culture. A short biographical note devoted to Ross G. Harrison, whose insight and pioneering work led to the foundation of animal cell culture is likewise included. Cell biologists and researchers who use in vitro techniques will find the book highly informative and insightful.

Contributors to Volume 2

Preface

Richard Benedict Goldschmidt

Cellular Senescence in Vitro

I. Introduction: Definitions of Aging

II. Historical Background

III. Characteristics of Cell Growth in Culture

IV. Cellular Morphology in Aging Cultures

V. Macromolecular Synthesis

VI. Proposed Mechanisms of Cellular Senescence

VII. Factors Modulating Replicative Life Span

VIII. Conclusions

References

Introduction and Expression of Foreign DNA Sequences in Mammalian Cells

I. Introduction

II. Methods of Introducing Genes

III. Vectors for Introduction of DNA

IV. Factors That Affect Gene Transfer

V. Fate of DNA in the Recipient Cell

VI. Transfer of Selectable and Nonselectable Genes

VII. Expression of Foreign Genes in Mammalian Cells

VIII. Rescue and Recovery of DNA Sequences

IX. Future of DNA Transfer Systems

References

Detection of Mycoplasmal Infection of Cell Cultures

I. Introduction

II. Methods to Detect Cell Culture Mycoplasmas

III. Mycoplasmal Infection of Differentiated Cell Cultures

IV. Identification of Isolates

V. Frequency of Mycoplasmal Assays

VI. Conclusion

References

Media for Insect Cell Cultures

I. Introduction

II. Nutritional Requirements and Constituents of Media

III. Media with Hemolymph or Sera

IV. Media Free of Hemolymph or Sera

V. Application of Vertebrate Culture Media to Insect Cell Cultures

VI. Epilogue

References

Insect Ovaries and Follicles in Culture: Oocyte and Early Embryonic Development in Pedogenetic Gall Midges

I. Introduction

II. Test Systems and Culture Techniques

III. Regular Oogenesis and Early Embryonic Development

IV. Irregular Oogenesis and Early Embryonic Development

V. Summary and Conclusions

References

Morphogenesis in Insect Tissue Culture

I. Introduction

II. Cell Culture

III. Organ Culture

IV. Conclusions

References

Agricultural Applications of in Vitro Plant Cultivation

I. Introduction

II. Current Status

III. Future Applications

IV. Changing Philosophy in Agricultural Research

References

Fish Cell Culture

I. Introduction

II. Physiological Salines

III. Culture Media

IV. Methods

V. Applications of Fish Cell Cultures

References

Index

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

Health

Advances in Cell Culture

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Karl Maramorosch