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A Practical Guide to Protein and Peptide Purification for Microsequencing
2nd Edition - May 20, 1993
Editor: Paul T. Matsudaira
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Why a Second Edition?The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than… Read more
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Why a Second Edition?The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
Mass spectrophotometric characterization of proteins and peptides
N-terminal sequencing of proteins with blocked N termini
Internal amino acid sequence analysis after protease digestion in-gel and on-blot
Improved microscale peptide purification methods
Computer analysis of protein sequences
New protocols tested and refined through everyday use in authors' laboratories
Updated reference chapter covering all aspects of protein microsequencing
Researchers and students at all levels in cell, developmental, and molecular biology, protein biochemistry and biophysics, neurobiology, pharmacology, immunology, parasitology, virology, genetics, plant science, biotechnology, and other biomedical/biological areas
Introduction. Strategies for Obtaining Partial Amino Acid Sequence Data from Small Quantities (5nmol) of Pure of Partially Purified Protein. Enzymatic Digestion of Proteins and HPLC Peptide Isolation. Purification of Proteins and Peptides By SDS-PAGE. Internal Amino Acid Sequence Analysis of Proteins After in Situ Protease Digestion on Nitrocellulose. Mass Spectrometric Strategies for the Structural Characterization of Proteins. Appendix. References. General Reference Books on Protein Microsequencing Methodologies.
No. of pages: 184
Published: May 20, 1993
Imprint: Academic Press
Paperback ISBN: 9780124802827
eBook ISBN: 9780080924618
Paul T. Matsudaira
Affiliations and expertise
Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, U.S.A.