A Practical Guide to Protein and Peptide Purification for Microsequencing
- 2nd Edition - May 20, 1993
- Latest edition
- Editor: Paul T. Matsudaira
- Language: English
Why a Second Edition?The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather tha… Read more
Description
Description
Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
Key features
Key features
- Mass spectrophotometric characterization of proteins and peptides
- N-terminal sequencing of proteins with blocked N termini
- Internal amino acid sequence analysis after protease digestion in-gel and on-blot
- Improved microscale peptide purification methods
- Computer analysis of protein sequences
- New protocols tested and refined through everyday use in authors' laboratories
- Updated reference chapter covering all aspects of protein microsequencing
Readership
Readership
Researchers and students at all levels in cell, developmental, and molecular biology, protein biochemistry and biophysics, neurobiology, pharmacology, immunology, parasitology, virology, genetics, plant science, biotechnology, and other biomedical/biological areas
Table of contents
Table of contents
Introduction. Strategies for Obtaining Partial Amino Acid Sequence Data from Small Quantities (5nmol) of Pure of Partially Purified Protein. Enzymatic Digestion of Proteins and HPLC Peptide Isolation. Purification of Proteins and Peptides By SDS-PAGE. Internal Amino Acid Sequence Analysis of Proteins After in Situ Protease Digestion on Nitrocellulose. Mass Spectrometric Strategies for the Structural Characterization of Proteins. Appendix. References. General Reference Books on Protein Microsequencing Methodologies.
Review quotes
Review quotes
Reviews of the first edition:
"Like the evening network television news programs, this book gives a useful summary of the highlights (of protein chemistry) to enable the masses to go forth armed with enough information to survive in the world."—Cell
"Like the evening network television news programs, this book gives a useful summary of the highlights (of protein chemistry) to enable the masses to go forth armed with enough information to survive in the world."—Cell
Product details
Product details
- Edition: 2
- Latest edition
- Published: December 2, 2012
- Language: English
About the editor
About the editor
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Paul T. Matsudaira
Affiliations and expertise
Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, U.S.A.View book on ScienceDirect
View book on ScienceDirect
Read A Practical Guide to Protein and Peptide Purification for Microsequencing on ScienceDirect