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A Manual of Paper Chromatography and Paper Electrophoresis
- 2nd Edition - January 1, 1958
- Authors: Richard J. Block, Emmett L. Durrum, Gunter Zweig
- Language: English
- Hardback ISBN:9 7 8 - 1 - 4 8 3 2 - 3 1 0 1 - 3
- Paperback ISBN:9 7 8 - 1 - 4 8 3 2 - 5 4 2 6 - 5
- eBook ISBN:9 7 8 - 1 - 4 8 3 2 - 7 6 8 0 - 9
A Manual of Paper Chromatography and Paper Electrophoresis provides a comprehensive discussion of the techniques of paper chromatography and paper electrophoresis. The book is… Read more
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Request a sales quoteA Manual of Paper Chromatography and Paper Electrophoresis provides a comprehensive discussion of the techniques of paper chromatography and paper electrophoresis. The book is organized into two parts. Part I on paper chromatography provides a readily accessible source for some of the many uses and adaptations of paper chromatography. An effort has been made to write a practical manual in which tried and proved procedures, employing relatively simple equipment and available reagents, are summarized. Part II on paper electrophoresis discusses basic principles and methodology. The emphasis throughout has been on the separation of protein mixtures, particularly blood serum. This reflects the fact that it is in this particular application that paper electrophoresis has thus far not been challenged by paper chromatography, whereas many of the smaller molecules can be resolved equally well or better by the thus far more widely employed chromatographic procedures.
PrefacePart I. Paper Chromatography I. Introduction II. Theory of Paper Chromatography III. General Methods IV. Quantitative Methods V. Amino Acids, Amines, and Proteins Section I: Amino Acids and Peptides Section II: Amino Acid Derivatives Section III: Amines Section IV: Separation of Proteins VI. Carbohydrates Section I: General Procedure Section II: Simple Sugars and Disaccharides Section III : Oligo- and Polysaccharides Section IV: Sugar Phosphates and Other Substituted Sugars Section V: Miscellaneous Derived Sugars VII. Aliphatic Acids Section I: Volatile Aliphatic Acids (C1 — C9) Section II: Non-Volatile Water-Soluble Aliphatic Acids Section III: α-Keto Acids Section IV: Higher Fatty Acids Section V: Miscellaneous Lipids VIII. Steroids and Bile Acids Section I: Steroids Section II: Bile Acids IX. Purines, Pyrimidines and Related Substances Section I: General Directions Section II: Detailed Experimental Procedures X. Phenols, Aromatic Acids, andPorphyrins Section I: Phenols and Aromatic Acids Section II: Porphyrins XI. Miscellaneous Organic Substances Alcohols Ketones and Aldehydes Sulfa Drugs Barbiturates Adrenaline and Related Compounds Alkaloids Chlorogenic and Caffeic Acids Nicotinic Acid and Related Compounds Color Reagent for Isonicotinoyl Hydrazide Tryptophan and Indoleacetic Acid Derivatives Imidazoles Urea, Creatine, Creatinine, etc. Methylol Compounds Thioureas Phospholipids and Their Nitrogenous Constituents Miscellaneous Organic Bases Chloroplast, Anthraquinone, and Other Pigments Coumarins and Related Compounds Flavonoid Pigments Tannins Acridines Flavin Derivatives Natural Pterins and Synthetic Derivatives pH Indicators and Other Dyes Coal Tar Colors Photographic Developers Organic Insecticides Polyamides (Nylons) Natural Rubber and Resins Naphthoquinones Guanidine Derivatives XII. Antibiotics and Vitamins Section I: Antibiotics Section II: Vitamins Section III: Plant Growth Substances XIII. Inorganic SeparationsPart II. Paper Electrophoresis Introduction XIV. General Theory Adsorption Partition Chromatography Membrane Polarization XV. Methods General Considerations Closed Strip Method Open Strip Techniques Hanging Strip Method Summary XVI. Two Dimensional Technique Closed Sheet Method Hanging Sheet Method Combination of Electrophoresis and Chromatography XVII. Continuous Electrophoresis XVIII. Some Quantitative ConsiderationsAppendix Electrolytes Commonly Employed in Paper Electrophoresis Dyeing ProceduresBibliography Part I Part IIAuthor IndexSubject Index
- No. of pages: 722
- Language: English
- Edition: 2
- Published: January 1, 1958
- Imprint: Academic Press
- Hardback ISBN: 9781483231013
- Paperback ISBN: 9781483254265
- eBook ISBN: 9781483276809
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