Hormone Assay
- 1st Edition - January 1, 1950
- Editor: C. W. Emmens
- Language: English
- Hardback ISBN:9 7 8 - 1 - 4 8 3 2 - 3 1 9 3 - 8
- Paperback ISBN:9 7 8 - 1 - 4 8 3 2 - 5 5 1 8 - 7
- eBook ISBN:9 7 8 - 1 - 4 8 3 2 - 7 2 8 6 - 3
Hormone Assay considers assay methods in detail, focusing on vertebrate hormones. Statistics as applied to biological assay is another field in which recent advances have been… Read more
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Request a sales quoteHormone Assay considers assay methods in detail, focusing on vertebrate hormones. Statistics as applied to biological assay is another field in which recent advances have been phenomenal, although the application of modern methods to the design and analysis of many assays awaits full development. This simultaneous progress in the two fields of work has nevertheless provided a fruitful series of examples of how assays can be conducted in order to gain the greatest amount of information from the material available, and to express results in precise form. The book opens with a chapter on the use of and need for statistics in assays, followed by a chapter on insulin, which exemplifies some of the methods described. Subsequent chapters discuss assay methods for parathyroid hormone, adrenalin, pituitary lobe hormones, melanophore expanding hormone from the pituitary, gonadotropins, adrenocorticotropin, thyrotropic hormone, and lactogenic hormone. Also covered are assay procedures for mammogenic hormone, mammogenic hormone, androgens, and estrogens.
List Of Contributors
Preface
I. Statistical Methods
I. Introduction
II. Statistical Terminology and Procedure
III. Types of Response
IV. Assays Based on Quantitative Responses
V. Assays Based on Qualitative Responses
VI. Predicting Assay Requirements
References
II. Insulin
I. Introduction
II. The Standard Preparation
III. The Rabbit Method of Assay
IV. The Mouse Method of Assay
V. Comparison of Rabbit and Mouse Methods of Assay
VI. The Assay of Insulin in Blood
References
III. Parathyroid Hormone
I. Introduction
II. Possibilities of a Standard Preparation
III. The Unit of Parathyroid Activity
IV. Methods of Assay
References
IV. Biological and Chemical Assay of Adrenalin
I. Introduction
II. The Blood Pressure of a Cat or Dog
III. The Rabbit's Intestine
IV. Shaw's Chemical Method and Specific Test
V. The Straub Frog Heart
VI. The Perfused Frog Heart
VII. The Perfused Frog Blood Vessels
VIII. The Hen's Rectal Caecum
IX. The Perfused Rabbit's Ear
X. The Isolated Rat's Uterus
XI. The Fluorescent Reaction
XII. Other Chemical Methods
XIII. Noradrenalin
References
V. Posterior Pituitary Lobe Hormones
I. Introduction
II. Standard Preparation
III. Methods of Assay
References
VI. Biological Assay of the Melanophore Expanding Hormone from the Pituitary
I. Introduction
II. Brief Historical Note
III. Theoretical Considerations
IV. Practical Details
V. Performance of an Assay
VI. Sample Assays
VII. Accuracy to be Expected
VIII. Implications of Disproportionate 'B'/Pressor and 'B '/Oxytocic Ratios
IX. Assay of Material Subjected to Caustic Soda Treatment
X. Assay of 'B' in Urine, Blood, and Tissue Extracts
XI. The Question of Whether the Pars Intermedia Manufactures Two Separate Chromatophore Excitants
XII. Summary
Appendix
References
VII. Gonadotropins
I. Historical Introduction
II. Site of Production and Classification of Gonadotropins
III. General Principles for the Assay of Gonadotropins
IV. Assay of Chorionic Gonadotropin
V. Assay of Pregnant Mares' Serum Gonadotropin
VI. Assay of Hypophyseal Gonadotropins
VII. Summary and Conclusions
References
VIII. Adrenocorticotropin
I. The Bioassay of ACTH in the Intact Animal
II. The Bioassay of ACTH in the Hypophysectomized Animal
References
IX. Thyrotropic Hormone
I. Introduction
II. Factors Influencing the Sensitivity of the Thyroid Gland
III. Methods of Assay of Thyrotropic Hormone
Addenda
References
X. Lactogenic Hormone
I. Introduction
II. Mammalian Assay Methods
III. Pigeon Assay Methods
IV. Comparison of Assay Methods Using International Standard Lactogenic Hormone
V. Comparison of Lactogenic Hormone Content in Pituitaries of Various Mature Animals
VI. Comparison of Lactogenic Hormone Content in Pituitaries of Various Animals as Affected by Pregnancy and Lactation, Suckling, and Following Estrogen Stimulation
References
XI. Mammogenic Hormone
I. Introduction
II. Preparation of Whole Mounts of Mammary Glands
III. Assay of Duct Growth
IV. Assay of Lobule-Alveolar Growth
V. Summary
References
XII. Growth Hormone
I. Introduction
II. Well-Established Procedures for the Bioassay of Growth Hormone
III. Suggested Methods for t he Bioassay of Growth Hormone
IV. Comment on Methods
References
XIII. Androgens
I. Introduction
II. Surgical Procedures
III. Bird Methods
IV. Mammalian Assays
V. Summary of Methods
References
XIV. Adrenal Cortical Hormones
I. Introduction
II. Adrenalectomy
III. Survival-Growth Methods
IV. Electrolyte Methods
V. Carbohydrate Methods
VI. Stress Tests
VII. Summary
References
XV. The Chemical Assay of Steroids of the Androgen and Adrenocortical Hormone Groups
I. Introduction—Chemical Structure of the Androgen and Adrenocortical Hormone Groups in Relation to Assay Methods
II. The Androgen Group
III. The Adrenocortical Hormone Group—The Development of Extraction and Chemical Assay Methods
References
XVI. Estrogens
I. Introduction
II. Types of Estrogen
III. Assays Based on Vaginal Cornification (Allen-Doisy Tests)
IV. Modifications of the Allen-Doisy Test
V. Interpretation of Results with Allen-Doisy Tests
VI. Intravaginal Allen-Doisy Tests
VII. Assays Based on Uterine Weight
VIII. Assays Based on Vaginal Opening
References
XVII. Hormones of the Corpus Luteum
I. Progestogens
II. Relaxin
References
XVIII. Chemical Assay of Estrogens and Pregnanediol
I. Introduction
II. Estrogens
III. Pregnanediol
References
XIX. Thyroidal Substances
I. Introduction
II. Thyroid Standards and Routes of Administration
III. Assays Based on Elevation of the Metabolic Rate
IV. Assays Based on Loss in Body Weight
V. Growth Restoration in Thyroidectomized Animals
VI. Maintenance of Thyroid-Pituitary Balance in Thiouracil-Treated Animals
VII. Assays Based on Stimulation of Metamorphosis in Amphibian Tadpoles
References
XX. The Chemical Assay of Thyroxine and Other Substances with Thyroidal Activity
I. Introduction
II. Methods of Chemical Assay of Compounds Having Thyroidal Activity
III. The Determination of Iodine in Organic Combination
IV. Discussion
References
Index
- No. of pages: 568
- Language: English
- Edition: 1
- Published: January 1, 1950
- Imprint: Academic Press
- Hardback ISBN: 9781483231938
- Paperback ISBN: 9781483255187
- eBook ISBN: 9781483272863
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