Hormone Assay

1st Edition - January 1, 1950
Editor: C. W. Emmens
Language: English
Hardback ISBN:
9 7 8 - 1 - 4 8 3 2 - 3 1 9 3 - 8
Paperback ISBN:
9 7 8 - 1 - 4 8 3 2 - 5 5 1 8 - 7
eBook ISBN:
9 7 8 - 1 - 4 8 3 2 - 7 2 8 6 - 3

Hormone Assay considers assay methods in detail, focusing on vertebrate hormones. Statistics as applied to biological assay is another field in which recent advances have been… Read more

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Hormone Assay considers assay methods in detail, focusing on vertebrate hormones. Statistics as applied to biological assay is another field in which recent advances have been phenomenal, although the application of modern methods to the design and analysis of many assays awaits full development. This simultaneous progress in the two fields of work has nevertheless provided a fruitful series of examples of how assays can be conducted in order to gain the greatest amount of information from the material available, and to express results in precise form. The book opens with a chapter on the use of and need for statistics in assays, followed by a chapter on insulin, which exemplifies some of the methods described. Subsequent chapters discuss assay methods for parathyroid hormone, adrenalin, pituitary lobe hormones, melanophore expanding hormone from the pituitary, gonadotropins, adrenocorticotropin, thyrotropic hormone, and lactogenic hormone. Also covered are assay procedures for mammogenic hormone, mammogenic hormone, androgens, and estrogens.

List Of Contributors

Preface

I. Statistical Methods

I. Introduction

II. Statistical Terminology and Procedure

III. Types of Response

IV. Assays Based on Quantitative Responses

V. Assays Based on Qualitative Responses

VI. Predicting Assay Requirements

References

II. Insulin

I. Introduction

II. The Standard Preparation

III. The Rabbit Method of Assay

IV. The Mouse Method of Assay

V. Comparison of Rabbit and Mouse Methods of Assay

VI. The Assay of Insulin in Blood

References

III. Parathyroid Hormone

I. Introduction

II. Possibilities of a Standard Preparation

III. The Unit of Parathyroid Activity

IV. Methods of Assay

References

IV. Biological and Chemical Assay of Adrenalin

I. Introduction

II. The Blood Pressure of a Cat or Dog

III. The Rabbit's Intestine

IV. Shaw's Chemical Method and Specific Test

V. The Straub Frog Heart

VI. The Perfused Frog Heart

VII. The Perfused Frog Blood Vessels

VIII. The Hen's Rectal Caecum

IX. The Perfused Rabbit's Ear

X. The Isolated Rat's Uterus

XI. The Fluorescent Reaction

XII. Other Chemical Methods

XIII. Noradrenalin

References

V. Posterior Pituitary Lobe Hormones

I. Introduction

II. Standard Preparation

III. Methods of Assay

References

VI. Biological Assay of the Melanophore Expanding Hormone from the Pituitary

I. Introduction

II. Brief Historical Note

III. Theoretical Considerations

IV. Practical Details

V. Performance of an Assay

VI. Sample Assays

VII. Accuracy to be Expected

VIII. Implications of Disproportionate 'B'/Pressor and 'B '/Oxytocic Ratios

IX. Assay of Material Subjected to Caustic Soda Treatment

X. Assay of 'B' in Urine, Blood, and Tissue Extracts

XI. The Question of Whether the Pars Intermedia Manufactures Two Separate Chromatophore Excitants

XII. Summary

Appendix

References

VII. Gonadotropins

I. Historical Introduction

II. Site of Production and Classification of Gonadotropins

III. General Principles for the Assay of Gonadotropins

IV. Assay of Chorionic Gonadotropin

V. Assay of Pregnant Mares' Serum Gonadotropin

VI. Assay of Hypophyseal Gonadotropins

VII. Summary and Conclusions

References

VIII. Adrenocorticotropin

I. The Bioassay of ACTH in the Intact Animal

II. The Bioassay of ACTH in the Hypophysectomized Animal

References

IX. Thyrotropic Hormone

I. Introduction

II. Factors Influencing the Sensitivity of the Thyroid Gland

III. Methods of Assay of Thyrotropic Hormone

Addenda

References

X. Lactogenic Hormone

I. Introduction

II. Mammalian Assay Methods

III. Pigeon Assay Methods

IV. Comparison of Assay Methods Using International Standard Lactogenic Hormone

V. Comparison of Lactogenic Hormone Content in Pituitaries of Various Mature Animals

VI. Comparison of Lactogenic Hormone Content in Pituitaries of Various Animals as Affected by Pregnancy and Lactation, Suckling, and Following Estrogen Stimulation

References

XI. Mammogenic Hormone

I. Introduction

II. Preparation of Whole Mounts of Mammary Glands

III. Assay of Duct Growth

IV. Assay of Lobule-Alveolar Growth

V. Summary

References

XII. Growth Hormone

I. Introduction

II. Well-Established Procedures for the Bioassay of Growth Hormone

III. Suggested Methods for t he Bioassay of Growth Hormone

IV. Comment on Methods

References

XIII. Androgens

I. Introduction

II. Surgical Procedures

III. Bird Methods

IV. Mammalian Assays

V. Summary of Methods

References

XIV. Adrenal Cortical Hormones

I. Introduction

II. Adrenalectomy

III. Survival-Growth Methods

IV. Electrolyte Methods

V. Carbohydrate Methods

VI. Stress Tests

VII. Summary

References

XV. The Chemical Assay of Steroids of the Androgen and Adrenocortical Hormone Groups

I. Introduction—Chemical Structure of the Androgen and Adrenocortical Hormone Groups in Relation to Assay Methods

II. The Androgen Group

III. The Adrenocortical Hormone Group—The Development of Extraction and Chemical Assay Methods

References

XVI. Estrogens

I. Introduction

II. Types of Estrogen

III. Assays Based on Vaginal Cornification (Allen-Doisy Tests)

IV. Modifications of the Allen-Doisy Test

V. Interpretation of Results with Allen-Doisy Tests

VI. Intravaginal Allen-Doisy Tests

VII. Assays Based on Uterine Weight

VIII. Assays Based on Vaginal Opening

References

XVII. Hormones of the Corpus Luteum

I. Progestogens

II. Relaxin

References

XVIII. Chemical Assay of Estrogens and Pregnanediol

I. Introduction

II. Estrogens

III. Pregnanediol

References

XIX. Thyroidal Substances

I. Introduction

II. Thyroid Standards and Routes of Administration

III. Assays Based on Elevation of the Metabolic Rate

IV. Assays Based on Loss in Body Weight

V. Growth Restoration in Thyroidectomized Animals

VI. Maintenance of Thyroid-Pituitary Balance in Thiouracil-Treated Animals

VII. Assays Based on Stimulation of Metamorphosis in Amphibian Tadpoles

References

XX. The Chemical Assay of Thyroxine and Other Substances with Thyroidal Activity

I. Introduction

II. Methods of Chemical Assay of Compounds Having Thyroidal Activity

III. The Determination of Iodine in Organic Combination

IV. Discussion

References

Index

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

Health

Hormone Assay

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