Bioassay

1st Edition - October 22, 2013
Imprint: Academic Press
Editor: Ralph I. Dorfman
Language: English
Paperback ISBN:
9 7 8 - 1 - 4 8 3 2 - 5 3 5 7 - 2
Hardback ISBN:
9 7 8 - 1 - 4 8 3 2 - 3 0 3 2 - 0
eBook ISBN:
9 7 8 - 1 - 4 8 3 2 - 7 2 7 6 - 4

Methods in Hormone Research, Volume II: Bioassay focuses on the detailed and critical account of bioassay methods, including electrolyte and carbohydrate methods, hormones, and… Read more

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Methods in Hormone Research, Volume II: Bioassay focuses on the detailed and critical account of bioassay methods, including electrolyte and carbohydrate methods, hormones, and assays. The selection first offers information on the statistical methods and steroid hormones and related substances. Topics include assays based on graded and quantal responses, measuring responses, types of estrogen, vaginal cornification, vaginal mitosis and epithelial thickness, and uterine weight. The book also ponders on anti-estrogenic compounds, progestational substances, and anti-gonadotropic steroids. The publication takes a look at androgens and anabolic agents, anti-androgenic substances, and corticoids. Discussions focus on surgical procedures, mammalian assays, bird, chick comb, electrolyte, and carbohydrate methods, adrenalectomy, and stress tests. The text then examines protein hormones and related hormones and substances, thyroidal substances, insulin, glucagon, and gonadotropins. The selection is a valuable source of data for readers interested in bioassays.

Contents

Contributors to Volume II

Preface

Contents, Volume I

Part I Statistical Methods

1. Statistical Methods

I. Introduction

II. Terminology and Procedure

III. Measuring Responses

IV. Assays Based On Graded Responses

V. Assays Based On Quantal Responses

VI. The Design of Assays

References

Part II Steroid Hormones and Related Substances

2. Estrogens

I. Introduction

II. Types of Estrogen

III. Vaginal Cornification

IV. Modifications of the Allen-Doisy Test

V. Interpreting Allen-Doisy Tests

VI. Intravaginal Allen-Doisy Tests

VII. Vaginal Mitosis and Epithelial Thickness

VIII. Vaginal Metabolic Activity

IX. Uterine Weight

X. Vaginal Opening

XI. In Vitro Assays

XII. Oral Dosage To Chicks

XIII. Duration of Action

XIV. Estrogen Inhibition

References

3. Anti-Estrogenic Compounds

I. Introduction

II. In Vivo Assays

III. In Vitro Assays

Iv. Conclusion

References

4. Progestational Substances

I. Introduction

II. Progestational Substances

III. Progestational Proliferation

IV. Endometrial Carbonic Anhydrase

V. Stromal Nuclear Hypertrophy

VI. Deciduoma Formation

VII. Pregnancy Maintenance and Parturition Delay

VIII. Anti-Fertility

IX. Chnical Evaluation of Progestational Agents

X. Concluding Remarks

References

5. Anti-Gonadotropic Steroids, Inhibition of Ovulation and Mating

I. Introduction

II. Parabiotic Technique

III. Development of the Parabiosis Method

IV. Inhibition of Gonadotropin In Intact Animals by Steroid Treatment

V. Reversal of Castration Changes In Pituitary Glands

VI. Inhibition of Luteinizing Hormone Activity of the Pituitary

VII. Inhibition of Ovulation

VIII. Inhibition of Fertility by Steroids

References

6. Androgens and Anabolic Agents

I. Introduction

II. Surgical Procedures

III. Bird Methods

IV. Mammahan Assays

V. Summary of Methods

References

7. Anti-Androgenic Substances

I. Introduction

II. Chick Comb Methods

III. Mammalian Methods

IV. Comments

References

8. Corticoids

I. Introduction

II. Adrenalectomy

Part III Protein Hormones and Related Hormones and Substances

9. Epinephrine and Norepinephrine

I. Introduction

II. Extraction and Separation

III. Bioassay

References

10. Thyroidal Substances

I. Introduction

II. Thyroidal Substances

III. Assays Based On Elevation of the Metabolic Rate

IV. Assays Based On Skeletal Changes

V. Assays Based On Goiter Prevention

VI. Assays Based On Use of Tadpoles

VII. Assays Based On Depression of Ii3i Uptake by Thyroid Gland

VIII. Methods of Determining Thyroxine Secretion Rate

IX. Thyroxine Secretion Rate

References

11. Insulin

I. Introduction

II. The Standard Preparation

III. The Rabbit Method of Assay

IV. The Mouse Method of Assay

V. Comparison of Rabbit and Mouse Methods of Assay

VI. Assay of Insulin At Physiological Levels

VII. Method Using Insulin Antiserum

VIII. Chromatographic Methods

References

12. Glucagon

I. Introduction

II. Methods of Assay

III. Measurement of Glucagon In Blood

IV. Summary

References

13. Parathyroid Hormone

I. Introduction

II. Possibilities of A Standard Preparation

III. The Unit of Parathyroid Activity

IV. Methods of Assay

References

14. Posterior Pituitary Hormones

I. Introduction

II. Standard Preparations

III. Methods of Assay

References

15. Melanophore-Expanding Activity

I. Introduction

II. Historical Resume

III. Theoretical Considerations

Iv. Methods Using The Whole Animal

V. Performance of An Assay

VI. Sample Assays

VII. Accuracy To Be Expected

VIII. Assay of Material Subjected To Heat at pH 12-13

IX. Methods Using Isolated Skin

X. Assay of Melanophore-Expanding Substance In Urine and Blood

References

16. Relaxin

I. Introduction

II. Standard Preparation

III. Guinea Pig Pubic Symphysis Methods of Assay

IV. Mouse Pubic Symphysis Methods of Assay

V. Assay of Relaxin by Inhibition of Uterine Motility

VI. Comparison of Relaxin Assay Methods

VII. Assay of Repository Forms of Relaxin

VIII. Other Actions of Relaxin Potentially Suitable For Bioassay Work

IX. Notes On The Stability of Relaxin

References

17. The Gonadotropins

I. Historical Introduction

II. Reference Standards

III. Use of Gonadotropin Assays

IV. Method of Urine Collection

V. Clinical Assay

VI. Bioassay

References

18. Prolactin

I. Historical Introduction

II. Assay Methods

III. Assay of Prolactin In Human Urine

References

19. Thyrotropic Hormone

I. Introduction

II. Factors Influencing The Sensitivity of the Thyroid Gland

III. Methods of Assay of Pituitary TSH

IV. Determination of TSH Secretion Rate

V. Bioassay of TSH In Blood Serum

Vi. Bioassay of TSH In Urine

References

20. Adrenocorticotropin

I. Introduction

II. Standard Preparations and Definition of Unit

III. In Vitro Assay Procedure (Saffran and Schally, 1955)

IV. Measurement of Corticosterone In Plasma and Adrenal Gland

V. Adrenal Ascorbic Acid As An End Point

VI. Other Assay Procedures

VII. Methods Other Than Ascorbic Acid

VIII. Choice of Procedure

References

21. Hypophyseal Growth Hormone

I. Introduction

II. Bioassay Based On Increment of Body Weight

III. The Tibia Test

IV. Immunological Tests

V. Miscellaneous Methods

VI. Concluding Remarks

References

Part IV Standard Methods

22. Standard Methods Adopted by Official Organizations

I. General Instructions For Hormone Assay Procedures

II. Specific Instructions For Hormone Assay Procedures

III. Interpretation of Biological Assay Data

Author Index

Subject Index

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

Health

Bioassay

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