Zebrafish at the Interface of Development and Disease Research

Chapter One: Modeling Syndromic Congenital Heart Defects in Zebrafish

• Abstract
• 1 Introduction
• 2 Zebrafish Heart Development
• 3 Zebrafish Models of Syndromic CHD
• 4 Conclusion
• Acknowledgments

Chapter Two: Using Zebrafish to Study Kidney Development and Disease

• Abstract
• 1 Introduction
• 2 Conserved Features of Vertebrate Kidney Development: Zebrafish as a Model System
• 3 Disease and Development: CAKUT in Zebrafish
• 4 Renal Ciliopathy Models in Zebrafish
• 5 Kidney Injury and Regeneration
• 6 Stem/Progenitor Cells in Kidney Repair and Regeneration
• 7 Using Zebrafish to Uncover Potential Treatments for AKI
• 8 Future Directions
• Acknowledgments

Chapter Three: Zebrafish Developmental Models of Skeletal Diseases

• Abstract
• 1 Introduction
• 2 Bone and Cartilage: Overview of Zebrafish Skeletal Development
• 3 Synthesis of the Cartilage Matrix Components
• 4 Processing of ECM Macromolecules
• 5 Zebrafish Models of Axial Skeleton Defects
• 6 Concluding Remarks
• Funding and Acknowledgments

Chapter Four: Netting Novel Regulators of Hematopoiesis and Hematologic Malignancies in Zebrafish

• Abstract
• 1 Introduction
• 2 Development of the Hematopoietic System in Zebrafish
• 3 Interrogating Hematopoiesis in the Zebrafish Model
• 4 Investigating Adult HSC Maintenance and Function
• 5 Modeling Hematological Diseases in Zebrafish
• 6 Concluding Remarks
• Acknowledgments

Chapter Five: Making It New Again: Insight Into Liver Development, Regeneration, and Disease From Zebrafish Research

• Abstract
• 1 Introduction
• 2 Liver Development: Zebrafish as a Paradigm
• 3 Liver Regeneration
• 4 Strengths and Challenges of Using Zebrafish to Study Liver Regeneration
• 5 Concluding Remarks: How Studying Liver Development and Regeneration Provides an Understanding of Liver Disease
• Acknowledgments

Chapter Six: “Muscling” Throughout Life: Integrating Studies of Muscle Development, Homeostasis, and Disease in Zebrafish

• Abstract
• 1 Introduction
• 2 From Mesoderm to Musculature: Overview of Skeletal Muscle Development
• 3 Trademark Traits of Skeletal Muscle: Muscle Cell Fusion and Sarcomeres
• 4 MTJ Development
• 5 From Development to Homeostasis
• 6 New Insights Into Muscle Disease From Zebrafish
• 7 Conclusions and Future Prospects
• Acknowledgments

Chapter Seven: Zebrafish Pancreas Development and Regeneration: Fishing for Diabetes Therapies

• Abstract
• 1 Introduction to the Pancreas
• 2 Gene-Function Analysis Streamlined in Zebrafish
• 3 Developing Renewable Sources of Beta Cells for Cell-Based Diabetes Therapies
• 4 Pancreas Regeneration as a Therapeutic Tool
• 5 Pancreas as a Metabolic Regulator: Studying Metabolic Disease in Zebrafish
• 6 Future Directions
• Acknowledgments

Chapter Eight: Modeling Infectious Diseases in the Context of a Developing Immune System

• Abstract
• 1 Introduction
• 2 Development of the Cell Types of the Innate Immune System
• 3 Genetic Control and Experimental Manipulation of the Zebrafish Innate Immune System
• 4 Pathways Required for Pathogen Recognition and Activation of the Innate Immune Response
• 5 Effects of Commensal Microbes on Development of the Immune System
• 6 Adaptation to Infection and Inflammation
• 7 The Interface of Immunity and Metabolism
• 8 Recent Insights From Modeling Infectious Diseases in Developing Embryos and Larvae
• 9 Concluding Remarks
• Acknowledgments

Chapter Nine: Zebrafish in Toxicology and Environmental Health

• Abstract
• 1 Introduction
• 2 Historical Perspective: Toxicology and Government Efforts for Environmental Regulation
• 3 Environmental and Developmental Toxicology
• 4 Zebrafish: Testing the Waters for Toxicants
• 5 Transcriptional Profiling to Identify Contaminants
• 6 High-Throughput Screening for Toxicity Studies
• 7 Assessing Health Impacts of Environmental Exposures Using Zebrafish
• 8 Long-Term and Transgenerational Effects of Toxin Exposures
• 9 Pathways and Mechanisms of Toxicant-Induced Disease
• 10 Limitations to the Zebrafish Model System
• 11 Conclusions
• Acknowledgments