S.G. Oliver, Introduction to Functional Analysis of the Yeast Genome. E.J. Louis, Whole Chromosome Analysis. H.W. Mewes, D. Frishman, A. Zollner, and K. Heumann, The Bioinformatics of the Yeast Genome. R.D.Gietz and R.A. Woods, Transformation of Yeast by the Lithium Acetate/Single-Stranded Carrier DNA/PEG Method. A. Wach, A. Brachat, C. Rebischung, S. Steiner, K. Pokorni, S. te Heesen, and P. Philippsen, PCR-based Gene Targeting in Saccharomyces cerevisiae. M.J.R. Stark, Studying Essential Genes–Generating and Using Promoter Fusions and Conditional Alleles. D.J. Garfinkel, M.J. Curcio, and V. Smith, Ty Mutagenesis. A.J.P. Brown, L.M. Furness, and D. Bailey, TranscriptAnalysis. N. Koloteva, J.M.X. Hughes, and J.E.G. McCarthy, Reporter Genes and Their Use in Studying Yeast Gene Expression. P. Ross-Macdonald, A. Sheehan, C. Friddle, G.S. Roeder, and M. Snyder, Transposon Tagging I: A Novel System for Monitoring Protein Production, Function and Localization. M. Bolotin-Fukuhara, Transposon Tagging II: Exploration of Gene Function and Regulatory Networks in Yeast with the Mini-Mu Transposon. I.M. Hagan and C.J. Stirling, Immunological Approaches tothe Study of Protein Localisation in Yeast. F.M. Klis, A.F.J. Ram, R.C. Montijn, J.C. Kapteyn, L.H.P. Caro, J.H. Vossen, M.A.A. Van Berkel, S.S.C. Brekelmans, and H. Van den Ende, Posttranslational Modifications of Secretory Proteins. M.J.R. Stark, Studying Post-Translational Modifications in Yeast–Intracellular Proteins. J. Rosamond, Two-Hybrid Analysis of Protein–Protein Interactions in Yeast. J. de la Cruz, M.C. Daugeron, and P. Linder, 'Smart Genetic Screens. B. Teusink, F. Baganz, H.V. Westerhoff, and S.G. Oliver, Metabolic Control Analysis as a Tool in the Elucidation of the Function of Novel Genes. W.H. Mager, K. Maurer, and P.W. Piper, Identifying Stress Genes. M.F. Tuite, I. Stansfield, and R.J. Planta, Identifying Genes Encoding Components of the Protein Synthesis Machinery of the Yeast Saccharomyces cerevisiae. M. Molina, H. Martan, M. Sanchez, and C. Nombela, MAP Kinase-Mediated Signal Transduction Pathways. P.T. Magee, TheAnalysis of the Candida albicans Genome. P.A. Moore, Applications of Yeast in the Discovery of Human Gene Function. K.D. Entian and P. Kitter, Yeast Mutant and Plasmid Collections. A. Brown and M.F. Tuite, Yeast Gene Analysis in the 21st Century. Appendices: R.J. Planta, I. Stansfield, and M.F. Tuite, The Ribosomal Protein Genes of Saccharomyces cerevisiae. A.J.P. Brown and M.F. Tuite, Yeast Growth. A.J.P. Brown, Useful World Wide Web Addresses for YeastResearchers. M.F. Tuite, Nomenclature for Yeast Genes and Proteins. Subject Index.