Time-Resolved Methods in Structural Biology
- 1st Edition, Volume 709 - November 27, 2024
- Imprint: Academic Press
- Editors: Peter Moody, Hanna Kwon
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 3 - 3 1 4 5 6 - 8
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 3 1 4 5 7 - 5
Time-Resolved Methods in Structural Biology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an intern… Read more
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Request a sales quoteTime-Resolved Methods in Structural Biology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
- Provides the latest information on biological research
- Offers outstanding and original reviews on a range of biological research topics
- Serves as an indispensable reference for researchers and students alike
Provides invaluable information on the fast-moving field of biological research that includes outstanding original reviews on a variety of topics
- Cover image
- Title page
- Table of Contents
- Series Page
- Copyright
- Contributors
- Preface
- Chapter One: The growth of microcrystals for time resolved serial crystallography
- Abstract
- 1 Introduction
- 2 Properties of macromolecular crystals
- 3 Microcrystals for time resolved serial crystallography
- 4 Screening and optimization
- 5 Supersaturation, nucleation and growth
- 6 Matching crystallization methods to the presentation of crystals to the X-ray beam
- 7 Creating a state of supersaturation
- 8 Precipitants
- 9 Visualization
- References
- Chapter Two: Use of fixed targets for serial crystallography
- Abstract
- 1 Introduction
- 2 Common aspects of SSX and SFX: optimising the experiment
- 3 Data collection
- 4 Record keeping, log and beamtime logistics – best practice
- 5 Summary
- Acknowledgements
- References
- Chapter Three: Sample efficient approaches in time-resolved X-ray serial crystallography and complementary X-ray emission spectroscopy using drop-on-demand tape-drive systems
- Abstract
- 1 Introduction
- 2 Materials
- 3 Methods
- References
- Chapter Four: Sample delivery for structural biology at the European XFEL
- Abstract
- 1 Introduction
- 2 Scientific instruments at the European XFEL
- 3 Sample delivery for SFX via liquid jets
- 4 High viscosity extrusion for SFX experiments
- 5 Testing of sample delivery
- 6 Fixed-target sample delivery
- 7 Drop-on-demand sample delivery
- 8 Summary and outlook
- References
- Chapter Five: Experimental approaches for time-resolved serial femtosecond crystallography at PAL-XFEL
- Abstract
- Abbreviations
- 1 Introduction
- 2 NCI experimental hutch
- 3 Sample environments for TR-SFX at the NCI experimental hutch
- 4 Sample preparation
- 5 Experimental setup for TR-SFX at NCI experimental hutch
- 6 Data processing
- 7 Safety considerations and others
- Acknowledgments
- References
- Chapter Six: Time-resolved IR spectroscopy for monitoring protein dynamics in microcrystals
- Abstract
- 1 Introduction
- 2 Overview of instrument
- 3 Experimental procedure
- 4 Results and discussion
- 5 Conclusion
- Acknowledgments
- References
- Chapter Seven: Multiplexing methods in dynamic protein crystallography
- Abstract
- 1 Introduction
- 2 Theory
- 3 Experimental design
- 4 Data processing
- 5 Conclusion and outlook
- Acknowledgments
- References
- Chapter Eight: Processing serial synchrotron crystallography diffraction data with DIALS
- Abstract
- 1 Introduction
- 2 Methods
- 3 DIALS command-line tools for processing SSX data
- 4 Xia2.ssx: an automated processing pipeline
- 5 Demonstration and evaluation on example data
- 6 Discussion
- Acknowledgments
- References
- Chapter Nine: Time-resolved scattering methods for biological samples at the CoSAXS beamline, MAX IV Laboratory
- Abstract
- 1 Introduction
- 2 Technical design of CoSAXS
- 3 SUrF—combined SAXS with UV–vis/Raman/fluorescence
- 4 Time-resolved SAXS studies using microfluidic chips
- 5 Structural kinetics investigated with stopped-flow
- 6 Time-resolved X-ray solution scattering, TR-XSS
- 7 Future perspectives
- Acknowledgments
- References
- Edition: 1
- Volume: 709
- Published: November 27, 2024
- No. of pages (Hardback): 412
- No. of pages (eBook): 412
- Imprint: Academic Press
- Language: English
- Hardback ISBN: 9780443314568
- eBook ISBN: 9780443314575
PM
Peter Moody
Peter Moody is Professor of Structural Biology at Leicester Institute for Structural & Chemical Biology, Department of Molecular & Cell Biology, University of Leicester, UK
Affiliations and expertise
University of Leicester, UKRead Time-Resolved Methods in Structural Biology on ScienceDirect