Tietz Textbook of Laboratory Medicine

[This is the current edition; approximately five chapters will be added, which will be determined by the author post-signing. The new chapters will either be e-only, or existing chapters will be moved to e-only; the author is on board with the print page reduction.] SECTION I: BASICS OF LABORATORY MEDICINE 1. Laboratory Medicine 2. Statistical Methodologies in Laboratory Medicine: Analytical and Clinical Evaluation of Laboratory Tests 3. Governance, Risk, and Quality Management in the Medical Laboratory 4. Specimen Collection and Processing 5. Preanalytical Variation and Pre-Examination Processes 6. Quality Control of the Analytical Examination Process 7. Standardization and Harmonization of Analytical Examination Results 8. Biological Variation and Analytical Performance Specifications 9. Establishment and Use of Reference Intervals 10. Evidence-Based Laboratory Medicine 11. Biobanking 12. Laboratory Support of Pharmaceutical, In Vitro Diagnostics, and Epidemiologic Studies 13. Machine Learning and Big Data in Laboratory Medicine 14. Laboratory Stewardship and Test Utilization 15. Principles of Basic Techniques and Laboratory Safety SECTION II: ANALYTICAL TECHNIQUES 16. Optical Techniques 17. Electrochemistry and Chemical Sensors 18. Electrophoresis 19. Chromatography 20. Mass Spectrometry 21. Sample Preparation for Mass Spectrometry 22. MALDI-TOF Mass Spectrometry Applications in Infectious Diseases 23. Development and Validation of Small Molecule Analytes by Liquid Chromatography-Tandem Mass Spectrometry 24. Proteomics 25. Enzyme and Rate Analysis 26. Immunochemical Techniques 27. Microfabrication and Microfluidics and Their Application in Clinical Diagnostics 28. Cytometry 29. Automation in the Clinical Laboratory 30. Point-of-Care Testing SECTION III: CLINICAL CHEMISTRY-ANALYTES 31. Amino Acids, Peptides, and Proteins 32. Serum Enzymes 33. Tumor Markers 34. Kidney Function Tests 35. Carbohydrates 36. Lipids and Lipoproteins 37. Electrolytes and Blood Gases 38. Hormones 39. Vitamins and Trace Elements 40. Iron Metabolism 41. Porphyrins and the Porphyrias 42. Therapeutic Drugs and Their Management 43. Clinical Toxicology 44. Toxic Elements 45. Body Fluids SECTION IV: CLINICAL CHEMISTRY-PATHOPHYSIOLOGY 46. Nutrition: Laboratory and Clinical Aspects 47. Diabetes Mellitus 48. Cardiac Function 49. Kidney Disease 50. Disorders of Water, Electrolytes, and Acid-Base Metabolism 51. Liver Disease 52. Gastric, Intestinal, and Pancreatic Function 53. Monoamine-Producing Tumors 54. Bone and Mineral Metabolism 55. Pituitary Function and Pathophysiology 56. Adrenal Cortex 57. Thyroid Disorders 58. Reproductive Endocrinology and Related Disorders 59. Pregnancy and Its Disorders SECTION V: GENETIC METABOLIC DISORDERS 60. Newborn Screening and Inborn Errors of Metabolism 61. Inborn Errors of Metabolism: Disorders of Complex Molecules SECTION VI: MOLECULAR DIAGNOSTICS 62. Principles of Molecular Biology 63. Nucleic Acid Isolation 64. Nucleic Acid Techniques 65. Genomes, Variants, and Massively Parallel Methods 66. Clinical Genome Sequencing 67. Molecular Microbiology 68. Genetics 69. Solid Tumors 70. Hematopathology 71. Circulating Tumor Cells and Circulating Nucleic Acids in Oncology 72. Circulating Nucleic Acids for Prenatal Diagnostics 73. Pharmacogenetics SECTION VII: HEMATOLOGY AND COAGULATION 74. Automated Hematology 75. Leukocyte Morphology in Blood and Bone Marrow 76. Red Blood Cell Morphology and Indices: The Clinical Chemistry Interface 77. Hemoglobin and Hemoglobinopathies 78. Enzymes of the Red Blood Cell 79. Physiology of Hemostasis 80. Platelets and von Willebrand Factor 81. Coagulation, Anticoagulation, and Fibrinolysis SECTION VIII: MICROBIOLOGY 82. Introduction to Infectious Diseases 83. The Role of the Clinical Laboratory in Infection Prevention and Antimicrobial Stewardship 84. Bacteriology 85. Antimicrobial Susceptibility Testing 86. Mycobacteriology 87. Mycology 88. Parasitology 89. Virology SECTION IX: TRANSFUSION MEDICINE 90. Blood Group Systems and Pretransfusion Testing 91. Blood Components, Product Modifications, and Blood Donor Screening 92. Indications for Transfusion: RBCs, Platelets, Plasma, and Cryoprecipitate 93. Transfusion Reactions and Adverse Events Associated With Transfusion SECTION X: CLINICAL IMMUNOLOGY 94. Systemic Autoimmune Disease 95. Transplant, Solid Organ 96. Hematopoietic Cell Transplantation 97. Transplant Compatibility Testing 98. Monoclonal Antibody Therapeutics and Immunogenicity 99. Allergy Testing 100. Primary Immunodeficiencies and Secondary Immunodeficiencies APPENDICES Appendix A: Reference Information for the Clinical Laboratory Appendix B: Answers to Multiple Choice Questions