Three Phase Partitioning

Applications in Separation and Purification of Biological Molecules and Natural Products

1st Edition - August 11, 2021
Imprint: Elsevier
Editors: Munishwar Nath Gupta, Ipsita Roy
Language: English
Paperback ISBN:
9 7 8 - 0 - 1 2 - 8 2 4 4 1 8 - 0
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 8 5 9 0 3 - 5

Three Phase Partitioning: Applications in Separation and Purification of Biological Molecules and Natural Products presents applications in diverse areas of both chemical technolog… Read more

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Three Phase Partitioning: Applications in Separation and Purification of Biological Molecules and Natural Products presents applications in diverse areas of both chemical technology and biotechnology. This book serves as a single resource for learning about both the economical, facile and scalable processes, along with their potential for applications in the separation and purification of materials and compounds across the entire spectra of chemical and biological nature. The book begins by explaining the origins and fundamentals of TPP and continues with chapters on related applications, ranging from the purification of parasite recombinant proteases to oil extraction from oilseeds and oleaginous microbes, and more.

Cover Image
Title Page
Copyright
Table of Contents
Contributors
Preface
The journey in understanding interactions of salts and solvents with proteins continues!
Chapter 1 Three phase partitioning: some reminiscences, some science
Abstract
Chapter Outline
1.1 The origin of the tpp method
1.2 How does TPP work?
1.3 Inhibition of enzyme activity by t-BuOH
1.4 Enhanced activity
1.5 Other molecules
1.6 TPP doesn’t work in all cases
References
Chapter 2 How and why we happen to use three phase partitioning in areas other than protein purification
Abstract
Chapter Outline
2.1 TPP for purification of proteins/enzymes
2.2 TPP used for edible oil extraction
2.3 TPP of polysaccharides
2.4 TPP of microbial cells
2.5 Isolation and purification of low molecular weight compounds
2.6 Conclusion
References
Chapter 3 Fundamental aspects of protein isolation and purification
Abstract
Chapter Outline
3.1 Introduction
3.2 Fusion tags and protein solubility
3.3 Cell lysis
3.4 Non-mechanical procedures
3.5 Protein precipitation
3.6 Affinity precipitation and immunoprecipitation
3.7 Protein purification
3.8 Conclusions and outlooks
References
Chapter 4 The multiple facets of three-phase partitioning in the purification, concentration, yield and activity of enzymes and proteins
Abstract
Chapter Outline
4.1 Introduction
4.2 TPP as a rapid single step procedure to isolate and concentrate proteins
4.3 TPP concentrates proteins by decreasing the volume of water
4.4 TPP concentrates individual proteins by removing unwanted proteins
4.5 TPP preserves and increases enzyme activity
4.6 Explanations for TPP preserving and increasing enzyme activity
4.7 Purification and/or refolding of denatured enzymes with TPP
4.8 TPP purification of recombinant HIS-Tag fusion proteins or metal binding proteins
4.9 TPP purification of proteins with affinity ligands
4.10 Ultrasound assisted TPP to isolate proteins, oils and polysaccharides
4.11 Microwave assisted TPP
4.12 Versatility of TPP. separating DNA, carbohydrates and oils and two-step TPP protocols
4.13 Conclusion
References
Chapter 5 Enzymes recovery by three phase partitioning
Abstract
Chapter Outline
5.1 Introduction
5.2 Overview of the studies and conditions of use of three phase partitioning and its variants for the recovery of enzymes
5.3 Three phase partitioning for the recovery of glycosidases
5.4 Three phase partitioning for the recovery of proteases
5.5 Three phase partitioning for the recovery of oxidoreductases
5.6 Three phase partitioning for the recovery of lipases
5.7 Three phase partitioning for the recovery of other hydrolases
5.8 Conclusion
References
Chapter 6 Emulsion gel formation in three phase partitioning
Abstract
Chapter Outline
6.1 Introduction
6.2 The mechanism of TPP
6.3 Conclusion
References
Chapter 7 Three-phase partitioning (TPP) of proteases from parasites, plants, tissue and bacteria for enhanced activity
Abstract
Chapter Outline
7.1 Why we are interested in proteases
7.2 Three-phase partitioning as a protease purification tool
7.3 Conditions considered during optimization of tpp
7.4 Effect of tpp on protease structure and activity
7.5 Conclusions
References
Chapter 8 Three phase partitioning of plant peroxidases
Abstract
Chapter Outline
8.1 Peroxidases
8.2 Sources and functions of peroxidases
8.3 Biotechnological applications of plant peroxidases
8.4 Three phase partitioning system
8.5 TPP as an emerging technique for plant peroxidase purification
8.6 Conclusion
References
Chapter 9 Macro-(affinity ligand) facilitated three phase partitioningConverting TPP into an affinity based process
Abstract
Chapter Outline
9.1 Introduction
9.2 Water soluble polymers and smart polymers
9.3 Smart biocatalysts
9.4 MLFTPP
9.5 Conclusion/future perspectives
References
Chapter 10 Applications of three phase partitioning and macro-(affinity ligand) facilitated three phase partitioning in protein refolding
Chapter Outline
10.1 Introduction
10.2 Conclusion
References
Chapter 11 Three phase partitioning-based strategies for highly efficient separation of bioactive polysaccharides from natural resources
Abstract
Chapter Outline
11.1 Introduction
11.2 Factors affecting the TPP process for extraction of PSs
11.3 Process intensification of TPP system for PSs extraction
11.4 TPP combined with downstream techniques
11.5 TPP separation influencing the properties of PSs
11.6 Conclusions
Acknowledgements
References
Chapter 12 Technologies for oil extraction from oilseeds and oleaginous microbes
Abstract
Chapter Outline
12.1 Introduction
12.2 Importance of oil and lipid extraction
12.3 Green solvents and techniques for oil and lipid extraction
12.4 Green solvents for oil/ lipid extraction
12.5 Conventional and green extraction techniques for oil/ lipid extraction
12.6 Conclusion
Author’s contributions
Competing interest
Acknowledgements
References
Chapter 13 Three phase partitioning (TPP) as an extraction technique for oleaginous materials
Abstract
Chapter Outline
13.1 Introduction
13.2 Conventional extraction techniques for oleaginous material
13.3 Mechanism of extraction using TPP
13.4 Advantages of TPP
13.5 Factors affecting TPP
13.6 Hyphenated TPP-techniques
13.7 Challenges and future perspectives
References
Chapter 14 Intensification of extraction of biomolecules using three-phase partitioning
Abstract
Chapter Outline
14.1 Introduction
14.2 Key factors affecting the TPP method
14.3 Advanced TPP processes
14.4 Process intensification of TPP
14.5 Application of TPP for extraction and purification of biomolecules
14.6 Challenges in TPP
14.7 Conclusions
References
Index

Munishwar Nath Gupta

Dr. Munishwar Nath Gupta earned his PhD from Indian Institute of Science, Bengaluru, and completed post-doctoral positions at Massachusetts Institute of Technology (USA), University of Minnesota (USA), Lund University (Sweden), and University of Technology of Compiegne (France). He has taught chemistry, biochemistry, and biotechnology at Indian Institute of Technology, Delhi, between 1975-2016. He was awarded the National Science Talent fellowship (India) and Fellowships of National Academy of Sciences and Indian National Science Academy. He has edited three books on thermostability of enzymes, non-aqueous enzymology and affinity-based separation methods (published by Springer/Birkhauser). He was an Associate Editor of Biocatalysis and Biotransformation (Taylor and Francis) and founding and former editor-in-chief of Sustainable Chemical Processes (Springer). He’s served on editorial boards of several national and international journals and acted as a consultant to Novozyme (Denmark), Dabur (India), and other international companies. His research interests include applied biocatalysis and interfaces of biochemistry with nanotechnology.

Affiliations and expertise

Formerly post-doctoral positions: Massachusetts Institute of Technology, USA, University of Minnesota, USA, Lund University, Sweden, University of Technology of Compiegne, France

Ipsita Roy

Dr. Ipsita Roy earned her PhD from Indian Institute of Technology, Delhi, completed a post-doctoral position at Indian Institute of Technology, Delhi, and conducted an Alexander von Humboldt research fellowship at the University of Bonn (Germany). She has been teaching biotechnology and biochemistry at the postgraduate level at National Institute of Pharmaceutical Education and Research, S.A.S. Nagar, since 2005. Her research interests include stabilization of pharmaceutically important and disease-relevant proteins; aptamer technology; downstream processing of proteins.

Affiliations and expertise

Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), India

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

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