The Zebrafish: Cellular and Developmental Biology, Part B Developmental Biology

Chapter 1. Methods to study maternal regulation of germ cell specification in zebrafish

• Introduction
• 1. Stages of Maternal Germ Plasm Regulation and Specification
• 2. Studying the Dynamic Localization of Germ Plasm RNAs
• 3. Studying Molecular Requirements Through Loss-of-Function Approaches
• 4. Utility of Transgenic Fish to Study Germ Cell Development
• Conclusion

Chapter 2. Patterning, morphogenesis, and neurogenesis of zebrafish cranial sensory placodes

• Introduction
• 1. Specification of the PPR and Patterning the PPR into Specific Placodes
• 2. Cranial Placode Morphogenesis and Neurogenesis
• Conclusions

Chapter 3. Oligodendrocyte differentiation

• Introduction
• 1. Zebrafish As a Model to Study Oligodendrocyte Differentation
• 2. Insights Into Oligodendrocyte Development and Myelination Using Zebrafish
• 3. Zebrafish As Model to Study Remyelination
• Future Directions and Conclusions

Chapter 4. Studying the peripheral sympathetic nervous system and neuroblastoma in zebrafish

• Introduction
• 1. The Peripheral Autonomic Nervous System
• 2. The Zebrafish as a Model System for Studying PSNS Development
• 3. Zebrafish as a Novel Model for Studying Neuroblastoma
• Conclusion

Chapter 5. Zebrafish as a model for understanding enteric nervous system interactions in the developing intestinal tract

• Introduction
• 1. Genetic and Environmental Interactions During Enteric Nervous System Development
• 2. Interactions Between the Enteric Nervous System and the Immune System
• 3. Interactions Between the Enteric Nervous System and Effector Cells
• 4. Future Prospects

Chapter 6. Methods to study the development, anatomy, and function of the zebrafish inner ear across the life course

• Introduction
• 1. Imaging Methods for Analysis of the Zebrafish Ear and Lateral Line
• 2. Experimental Manipulation of Otic Development
• 3. Dissection and Imaging of the Adult Ear and Otoliths
• 4. Behavioral Analysis
• 5. Small Molecule Screening
• 6. Zebrafish Models of Human Deafness and Vestibular Disorders
• 7. Future Directions

Chapter 7. Imaging collective cell migration and hair cell regeneration in the sensory lateral line

• Introduction
• 1. Lateral Line Development
• 2. In Vivo Imaging of Lateral Line Primordium Migration
• 3. Live Labeling of Lateral Line Cells
• 4. Nonvital Tissue Labeling of Lateral Line Cells
• 5. Interpretation of Common Phenotypes
• 6. Hair Cell Regeneration
• 7. Long-Term Time-Lapse Analyses of Regenerating Neuromasts
• Conclusions

Chapter 8. Analysis of the retina in the zebrafish model

• Introduction
• 1. Development of the Zebrafish Retina
• 2. Analysis of the Visual System in Wild Type and Mutants
• 3. Analysis of Gene Function in the Zebrafish Retina
• Summary

Chapter 9. Strategies for analyzing cardiac phenotypes in the zebrafish embryo

• Introduction
• 1. Regulation of Heart Size
• 2. Regulation of Cardiac Morphology
• 3. Regulation of Cardiac Function
• 4. Summary

Chapter 10. Chemical approaches to angiogenesis in development and regeneration

• Introduction
• 1. High-Throughput Screening for Small Molecules With Proangiogenic Activity Using Primary Cell Culture of Tg[flk:GFP] Zebrafish Embryos
• 2. Validation of Proangiogenic Compounds in Vivo Using Pre-inhibited Vascular Structure of Zebrafish Embryo by VRI
• 3. Summary
• 4. Method

Chapter 11. Quantitative methods for studying hemostasis in zebrafish larvae

• Introduction
• 1. Methods
• Conclusions

Chapter 12. Zebrafish kidney development

• Introduction
• 1. Structure of the Zebrafish Pronephros
• 2. Formation of the Pronephros
• 3. Methods to Study Pronephros Function
• Conclusions

Chapter 13. Zebrafish pancreas as a model for development and disease

• Introduction
• 1. Molecular Mechanisms of Secondary Islet Formation
• 2. Physiology and Assessment of Glucose Homeostasis in Zebrafish
• 3. Diabetes and Related Disease Models in Zebrafish
• 4. Methods to Study Beta Cell Biology and Physiology
• 5. Future Directions

Chapter 14. Endoderm specification and liver development

• Introduction
• 1. Review of the Literature
• 2. Embryonic and Larval Protocols to Analyze Liver Formation
• 3. Liver Injury and Regeneration Protocols
• Summary

Chapter 15. Emerging tools to study proteoglycan function during skeletal development

• Introduction
• 1. Xylose: Where the “Proteo-” meets the “-Glycan”
• 2. Adding Sugar Like a Kid After Halloween
• 3. Don't Be a Quitter: Posttranslational Modifications of Posttranslational Modifications
• 4. Break it Down for Me, Fellas
• 5. Adding Function to Structure by Understanding PG-Loss Animal Models
• 6. Here's Looking at You, PG
• Conclusion

Chapter 16. Generation and analysis of zebrafish melanoma models

• Introduction
• 1. Genetic Models of Melanoma in Zebrafish
• 2. Cell Line Models of Melanoma in Zebrafish
• 3. Analysis of Melanomas
• 4. Perspectives

Chapter 17. Learning and memory in zebrafish (Danio rerio)

• Introduction
• Summary

Chapter 18. Working with zebrafish at postembryonic stages

• Introduction
• 1. Part I: Postembryonic Staging
• 2. Part II: Rearing Fish for Use at Postembryonic Stages
• 3. Recipes