The Zebrafish: Cellular and Developmental Biology, Part A Cellular Biology

Chapter 1. Embryonic cell culture in zebrafish

• Introduction
• 1. Methods
• Conclusion

Chapter 2. Cellular dissection of zebrafish hematopoiesis

• Introduction
• 1. Zebrafish Hematopoiesis
• 2. Hematopoietic Cell Transplantation
• 3. Enrichment of HSCs
• 4. In Vitro Culture and Differentiation of Hematopoietic Progenitors
• Conclusions

Chapter 3. Second harmonic generation microscopy in zebrafish

• Introduction
• 1. Materials
• 2. Methods
• 3. Notes
• Supplementary videos
• Supplementary Data

Chapter 4. Imaging blood vessels and lymphatic vessels in the zebrafish

• Introduction
• 1. Imaging Vascular Gene Expression
• 2. Nonvital Blood Vessel and Lymphatic Vessel Imaging
• 3. Vital Imaging of Blood and Lymphatic Vessels
• Conclusion

Chapter 5. An eye on light-sheet microscopy

• Introduction
• 1. Principle Behind Selective Plane Illumination Microscopy
• 2. The Microscope for Your Sample or the Sample for Your Microscope?
• 3. Data Acquisition and Handling
• 4. Challenges and Perspectives

Chapter 6. Single neuron morphology in vivo with confined primed conversion

• Introduction
• 1. Photoconvertible Fluorescent Proteins
• 2. Confined Primed Conversion
• 3. Unraveling Single Neuron Morphology With Confined Primed Conversion
• Conclusion and Outlook

Chapter 7. Visualizing retinoic acid morphogen gradients

• Introduction
• 1. Challenges for Morphogen Gradient Studies
• 2. Feedback Allows Retinoic Acid to Act as a Graded Morphogen
• 3. Cyp26s as Key Regulators of Retinoic Acid Gradient Formation
• 4. Visualizing the Retinoic Acid Gradient
• 5. Crabps and Retinoic Acid Signal Robustness
• 6. Sharpening Boundaries of Gene Expression in Response to Retinoic Acid Gradients
• 7. Noise—Both Good and Bad
• 8. Other Boundaries and Other Morphogens
• Conclusions and Perspectives

Chapter 8. Using fluorescent lipids in live zebrafish larvae: From imaging whole animal physiology to subcellular lipid trafficking

• Introduction
• 1. Forward Genetic Screening With Fluorescent Lipids
• 2. Visualizing Lipid Metabolism Using BODIPY Fatty Acid Analogs
• Summary

Chapter 9. Analysis of cilia structure and function in zebrafish

• Introduction
• 1. Cilia in Zebrafish Organs
• 2. Analytical Tools for Cilia Morphology and Motility
• 3. Phenotypes of Cilia Mutants in Zebrafish
• 4. Future Directions

Chapter 10. Functional calcium imaging in zebrafish lateral-line hair cells

• Introduction
• 1. Calcium Indicator Selection and Comparison
• 2. Imaging Systems and Optimal Parameters
• 3. Image Processing
• Summary
• Discussion

Chapter 11. Physiological recordings from the zebrafish lateral line

• Introduction
• 1. Common Methods for Lateral Line Electrophysiology
• 2. Stimulation of Neuromast Hair Cells
• 3. Recording Microphonic Potentials
• 4. In Vivo Hair Cell Physiology
• 5. Afferent Neuron Action Currents
• 6. Summary
• Discussion