The Recombination of Genetic Material

Contributors

Preface

I. Genetic Recombination: A Brief Overview

I. What Is Genetic Recombination?

II. What Are the Major Types of Recombination?

III. How Is Recombination Detected?

IV. How Often Does Recombination Occur, and How Long Does It Take?

V. What Length of DNA Can Be Involved in a Recombination Event, and How Much Is Necessary?

VI. What Indicates That Recombination Is Not "Simple"?

VII. How Is Recombination Important for Cellular Behavior?

VIII. A Recombination Bibliography

References


2. Recombination and Gene Conversion in Ascobolus

I. Introduction

II. Ascobolus as a Tool for Studying Genetic Recombination

III. NMS Patterns and Mutations

IV. Dual Origin of 6:2 Convertant Asci

V. Hybrid DNA Formation and Distribution

VI. Discussion and Conclusion

References


3. Genetic Analysis of Intragenic Recombination in Drosophila

I. Introduction

II. Experimental System

III. Results

IV. Discussion

References


4. Methyl-Directed Repair of DNA Mismatches

I. Introduction

II. Mismatch Repair in Recombination Models

III. Mismatch Repair in Escherichia coli

IV. Methyl-Directed Mismatch Repair

V. Experiments with Highly Methylated DNA Chains

VI. Methyl-Independent Mismatch Repair

VII. Nucleotide Specificity of Mismatch Repair

VIII. Methyl Direction and Other Repair Systems Specific for New DNA Chains

References


5. Homologous Recombination Sites and Their Recognition

I. Singularities in Homologous Recombination

II. Recombination Sites in Bacteria and Bacteriophage

III. Recombination Sites in Fungi

IV. Summary and Concluding Remarks

References


6. Pathways and Systems of Homologous Recombination in Escherichia coli

I. Introduction

II. Pathways of Recombination Defined by Conjugational Systems

III. Effects of rec Mutations on Other Recombination Systems

IV. Recombination Stimulation and rec Gene Dependence

V. Summing Up

References


7. Genetic Recombination: Molecular Biology, Biochemistry, and Evolution

I. Introduction

II. The Prototype Holliday Model

III. Physical Evidence for the Existence of the Holliday Recombination Intermediate

IV. Prokaryotic and Eukaryotic Recombination

V. Initiation Mechanisms for Forming the Holliday Recombination Intermediate

VI. The RecA Protein and Genetic Recombination

VII. Auxiliary Proteins Involved in Recombination

VIII. An Enzymatic Overview of the Recombination Mechanism

IX. On the Possible Evolution of the Recombination Mechanism

References


8. Transpositional and Site-Specific Recombination Mediated by Bacterial Transposons

I. Introduction

II. Tn3 and Related Transposons

III. IS Elements and Composite Transposons

IV. Bacteriophages Mu and D108

V. Unclassified Transposons

VI. Transposition Mechanisms

VII. Cointegrate Resolution and Related Site-Specific Recombination Systems

VIII. Addendum

References


9. Viral and Cellular Control of Site-Specific Recombination

I. Introduction

II. The Integration and Excision of Phage λ

III. Recombination Sites

IV. Proteins that Catalyze Site-Specific Recombination

V. Regulation of Site-Specific Recombination

VI. The Lysis-Lysogeny Decision

VII. Cellular Regulation of Site-Specific Recombination

References


10. Recombination between Repeated Yeast Genes

I. Introduction

II. Classes of Repeated Yeast Genes

III. General Homologous Recombination Events among Repeated Genes

IV. Specialized Homologous Recombination Events

V. Nonhomologous Recombination Events

VI. Implications of the Genetic Behavior of Repeated Yeast Genes

References


11. Recombination of Immunoglobulin Genes

I. Introduction

II. Immunoglobulin Structure

III. Immunoglobulin Genes

IV. General Features of Recombination

V. Recombination Mechanisms

VI. Static and Dynamic Systems

VII. Addendum

References


12. Induction of Recombination-Related Functions (SOS Functions) in Response to DNA Damage

I. Introduction

II. Conditions under Which SOS Functions Are Induced

III. Early Molecular Events Leading to the Induction of SOS Functions

IV. RecA Protein and the Expression of SOS Genes

V. SOS-Like Reactions in Lower Eukaryotes

VI. Possible SOS Functions in Mammalian Cells

VII. Concluding Remarks

References

Index